Oil Extraction from Rana catesbeiana by Supercritical Carbon Dioxide and Mechanical Pressing

Abstract

Oil extraction from the fat body of bullfrog (FBB) using supercritical carbon dioxide through supercritical fluid extraction (SFE) and mechanical pressing (ME) was assessed. ME extraction was performed within a temperature range from 40 to 90 C and the SFE at 40 C and 15 MPa. The peroxide index (PI) and oxidative induction time (OIT) of the oil obtained from the ME were negatively influenced by the increase in the extraction temperature; however, the FBB oil extracted at 40 C presented a high quality with acidity index and PI values of 4.7 mg KOH g −1 and 0.50 mEq kg −1 , respectively. The oils obtained from both processes presented similar physicochemical characteristics, especially related to the composition. Significant amounts of ω-3 (≈3.5%), ω-6 (≈19%), and ω-9 (≈50%) fatty acids were achieved. Thus, the valorization of the FBB through the recovery of its oil may be of great interest mainly due to the high yields achieved (66.7%).