Oil assessment of Halamphora coffeaeformis diatom growing in a hybrid two-stage system for biodiesel production

Martín, Lucas; Popovich, Cecilia A.; Damiani, M. Cecilia; Leonardi, Patricia I.

doi:10.1016/j.renene.2016.01.078

Cited by 31 publications

(37 citation statements)

References 36 publications

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“…In general, neutral lipids are the main lipid fraction found in diatoms [7,[21][22][23]. The production of TAG by H. coffeaeformis increased with the number of days in culture in the photobioreactor, and its value (in mg·L −1 ) was comparable to that reported for another diatom from Bahía Blanca Estuary grown under similar conditions [24].…”

Section: Discussionsupporting

confidence: 70%

“…In general, neutral lipids are the main lipid fraction found in diatoms [7,[21][22][23]. The production…”

Section: Discussionmentioning

confidence: 99%

“…The diatom Halamphora coffeaeformis, isolated from the Bahía Blanca Estuary [7], was maintained in f/2 medium with a salinity of 30, at 20 ± 1 • C and 40 µmol photons·m −2 ·s −1 under a 12:12 h light/ dark photoperiod. For experiments, a starting inoculum of 6.82 × 10 5 cells·mL −1 in f/2 medium was used.…”

Section: Experimental Designmentioning

confidence: 99%

“…Cells were counted daily in a Sedgwick-Rafter chamber, as described in [7]. The specific growth rate (µ) was estimated by a least square fit to a straight line of the logarithmically transformed data, according to [8].…”

Section: Growth Rate and Biomassmentioning

confidence: 99%

“…Fatty acids methyl esters (FAMEs) were obtained through transesterification using a potassium hydroxide solution. Then, they were analyzed in an HP 4890D gas chromatograph with a capillary SP2560 column (100 m, 0.25 mm, and 0.2 µm) (Supelco Inc., USA), a split/splitless injector and a flame-ionization detector, at 260 • C. Data were processed using the HP3398a GC Chemstation Software (Hewlett Packard, USA), and FAMEs were identified through comparison with the standard certificate material Supelco FAME 10 mix 37 (CRM47885, Sigma-Aldrich), as detailed in [7,12].…”

Section: Fatty Acid Characterizationmentioning

confidence: 99%

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Assessment of Halamphora coffeaeformis Growth and Biochemical Composition for Aquaculture Purposes

Bilbao

Martín

Popovich

et al. 2020

JMSE

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The vast majority of the world's fishery by-products are utilized in the fish farming industry. However, due to the high cost and unsustainability of these by-products, alternative sources must be found. Marine diatoms produce important amounts of fatty acids, sterols, proteins and carbohydrates. In this work, we assessed the nutritional value of Halamphora coffeaeformis at the exponential growth phase, to determine its suitability for aquafeed. The strain was grown in a photobioreactor at 20 • C for 6 days. The production of proteins, lipids and carbohydrates was determined, and essential fatty acid, sterol and amino acid composition was assessed. The highest values of triacylglycerides (TAG), free and esterified sterols, proteins and carbohydrates were found after 6 days of growth in the photobioreactor. Fatty acid analysis by gas chromatography showed the presence of eicosapentaenoic (EPA) and arachidonic (ARA) in amounts similar to those for fish oils. In terms of sterols, a predominance of stigmasterol was observed, followed by cholesterol. The amino acid composition revealed 50% of them to be among those essential for fish and other aquatic animals. Finally, a suitable amount of carbohydrates was found in H. coffeaeformis cultures. Together, these findings support the use of H. coffeaeformis as an alternative and sustainable source for aquafeed to partially replace the use of fishery by-products.

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