Oil and eicosapentaenoic acid production by the diatom <i>Phaeodactylum tricornutum</i> cultivated outdoors in Green Wall Panel (GWP®) reactors

Rodolfi, Liliana; Biondi, Natascia; Guccione, Alessia; Bassi, Niccolò; D’Ottavio, Massimo; Arganaraz, Gimena; Tredici, Mario R.

doi:10.1002/bit.26353

Cited by 54 publications

(26 citation statements)

References 51 publications

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“…The FAME profile showed that P. tricornutum contained considerable amounts of PUFA (31.41% or 2.85 wt %) ( Table 2). P. tricornutum's PUFA production has been reported in outdoor cultures, and findings suggested that under nutrient-replete conditions the amount of PUFA can reach 60% [56]. However, as this study was conducted outdoors under nutrient replete conditions, the amounts of PUFA were lower than other studies, which could be explained by differences in temperature and light conditions.…”

Section: Fatty Acids Compositionscontrasting

confidence: 54%

Potential of Phaeodactylum tricornutum for Biodiesel Production under Natural Conditions in Chile

et al. 2017

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Diatoms are very diverse and highly productive organisms, found in a wide variety of environments. This study aims to analyze the growth and lipid composition of Phaeodactylum tricornutum, cultured in an outdoor pilot-scale bubble column photobioreactor under natural conditions in Chile for biodiesel production. Results showed that P. tricornutum cultures reached their highest biomass concentration (0.96 ± 0.04 kg m −3) after 14 days of culturing, at the stationary phase, with a volumetric productivity of 0.13 kg m −3 d −1. Biomass samples showed a total lipid content of 9.08 ± 0.38 wt %. The fatty acid methyl ester analysis revealed a composition of 24.39% C16-C18 fatty acids, 42.34% saturated fatty acids, 21.91% monounsaturated fatty acids and 31.41% polyunsaturated fatty acids. These findings suggest that P. tricornutum oil can be used as an alternative raw material for the production of biodiesel capable of meeting international quality standards.

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Section: Fatty Acids Compositionscontrasting

confidence: 54%

Potential of Phaeodactylum tricornutum for Biodiesel Production under Natural Conditions in Chile

et al. 2017

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“…However, the different reactor types, operation modes and cultivation conditions used in these studies make empirical comparisons difficult. The same GWP®-III reactor panels were used by Rodolfi et al [28] for cultivation of P . tricornutum during spring and summer in Florence, Italy.…”

Section: Discussionmentioning

confidence: 99%

“…tricornutum strain. When recalculating the panel-areal productivities for our strains with the volume (37–43 L) and panel-area (0.78 m 2 ) used in Rodolfi et al [28] , higher EPA productivities were achieved for strain Fito in all seasons (0.60, 0.45, and 0.45 g m − 2 d − 1 ) and for strain M28 and B58 in spring (0.41 and 0.47 g m − 2 d − 1 , respectively).…”

Section: Discussionmentioning

confidence: 99%

Comparing EPA production and fatty acid profiles of three Phaeodactylum tricornutum strains under western Norwegian climate conditions

et al. 2018

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Microalgae could provide a sustainable alternative to fish oil as a source for the omega-3 polyunsaturated fatty acids eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). However, growing microalgae on a large-scale is still more cost-intensive than fish oil production, and outdoor productivities vary greatly with reactor type, geographic location, climate conditions and microalgae species or even strains. The diatom Phaeodactylum tricornutum has been intensively investigated for its potential in large-scale production, due to its robustness and comparatively high growth rates and EPA content. Yet, most research have been performed in southern countries and with a single commercial P. tricornutum strain, while information about productivities at higher latitudes and of local strains is scarce. We examined the potential of the climate conditions in Bergen, western Norway for outdoor cultivation of P. tricornutum in flat panel photobioreactors and cultivated three different strains simultaneously, one commercial strain from Spain (Fito) and two local isolates (M28 and B58), to assess and compare their biomass and EPA productivities, and fatty acid (FA) profiles. The three strains possessed similar biomass productivities (average volumetric productivities of 0.20, 0.18, and 0.21 g L− 1 d− 1), that were lower compared to productivities reported from southern latitudes. However, EPA productivities differed between the strains (average volumetric productivities of 9.8, 5.7 and 6.9 mg L− 1 d− 1), due to differing EPA contents (average of 4.4, 3.2 and 3.1% of dry weight), and were comparable to results from Italy. The EPA content of strain Fito of 4.4% is higher than earlier reported for P. tricornutum (2.6–3.1%) and was only apparent under outdoor conditions. A principal component analysis (PCA) of the relative FA composition revealed strain-specific profiles. However, including data from laboratory experiments, revealed more significant differences between outdoor and laboratory-grown cultures than between the strains, and higher EPA contents in outdoor grown cultures.

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“…Four 40 L flat‐panel PBRs (Green Wall Panel®‐III; F&M Fotosintetica & Microbiologica S.r.l. ; Rodolfi et al, 2017) were inoculated with culture from the 25 L flat‐panel PBR described previously, to a maximum cultivation volume of 38 L (initial OD 750 of 0.2, approximately). The PBRs were firstly run at batch mode until they reach the concentrations under study: 0.4 g L −1 DW biomass for low biomass concentration level ( T. lutea OD 750 of 1; P. tricornutum OD 750 of 0.7), and 1.1 g L −1 DW biomass for high biomass concentration level ( T. lutea OD 750 of 3; P. tricornutum OD 750 of 2).…”

Section: Methodsmentioning

confidence: 99%

Production and monitoring of biomass and fucoxanthin with brown microalgae under outdoor conditions

Gao

Sá

Itd

et al. 2020

Biotech & Bioengineering

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The effect of light on biomass and fucoxanthin (Fx) productivities was studied in two microalgae, Tisochrysis lutea and Phaeodactylum tricornutum. High and low biomass concentrations (1.1 and 0.4 g L−1) were tested in outdoor pilot‐scale flat‐panel photobioreactors at semi‐continuous cultivation mode. Fluorescence spectroscopy coupled with chemometric modeling was used to develop prediction models for Fx content and for biomass concentration to be applied for both microalgae species. Prediction models showed high R2 for cell concentration (.93) and Fx content (.77). Biomass productivity was lower for high biomass concentration than low biomass concentration, for both microalgae (1.1 g L−1: 75.66 and 98.14 mg L−1 d−1, for T. lutea and P. tricornutum, respectively; 0.4 g L−1: 129.9 and 158.47 mg L−1 d−1, T. lutea and P. tricornutum). The same trend was observed in Fx productivity (1.1 g L−1: 1.14 and 1.41 mg L−1 d−1, T. lutea and P. tricornutum; 0.4 g L−1: 2.09 and 1.73 mg L−1 d−1, T. lutea and P. tricornutum). These results show that biomass and Fx productivities can be set by controlling biomass concentration under outdoor conditions and can be predicted using fluorescence spectroscopy. This monitoring tool opens new possibilities for online process control and optimization.

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Oil and eicosapentaenoic acid production by the diatom Phaeodactylum tricornutum cultivated outdoors in Green Wall Panel (GWP®) reactors

Cited by 54 publications

References 51 publications

Potential of Phaeodactylum tricornutum for Biodiesel Production under Natural Conditions in Chile

Potential of Phaeodactylum tricornutum for Biodiesel Production under Natural Conditions in Chile

Comparing EPA production and fatty acid profiles of three Phaeodactylum tricornutum strains under western Norwegian climate conditions

Production and monitoring of biomass and fucoxanthin with brown microalgae under outdoor conditions

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