Offspring born from chimeras reconstructed from parthenogenetic and in vitro fertilized bovine embryos

Boediono, Arief; Suzuki, Takahiro; Li, L.Y.; Godke, R.A.

doi:10.1002/(sici)1098-2795(199906)53:2<159::aid-mrd5>3.0.co;2-x

Cited by 38 publications

(17 citation statements)

References 45 publications

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“…Eutherians (placental mammals) are not capable of this form of reproduction. However, chimeras of parthenogenetic cells coupled with biparentally derived embryonic tissues have generated apparently normal offspring, and the parthenogenetic origin of several tissues has been confirmed in such chimeric animals (7). In a reported case of a human parthenogenetic chimera, contribution to several tissues has been demonstrated, including blood where 100% of the leukocytes were found to be of parthenogenetic origin (8).…”

mentioning

confidence: 99%

Nonhuman primate parthenogenetic stem cells

Vrana

Hipp

Goss

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

176

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Parthenogenesis is the biological phenomenon by which embryonic development is initiated without male contribution. Whereas parthenogenesis is a common mode of reproduction in lower organisms, the mammalian parthenote fails to produce a successful pregnancy. We herein describe in vitro parthenogenetic development of monkey (Macaca fascicularis) eggs to the blastocyst stage, and their use to create a pluripotent line of stem cells. These monkey stem cells (Cyno-1 cells) are positive for telomerase activity and are immunoreactive for alkaline phosphatase, octamer-binding transcription factor 4 (Oct-4), stage-specific embryonic antigen 4 (SSEA-4), tumor rejection antigen 1-60 (TRA 1-60), and tumor rejection antigen 1-81 (TRA 1-81) (traditional markers of human embryonic stem cells). They have a normal chromosome karyotype (40 + 2) and can be maintained in vitro in an undifferentiated state for extended periods of time. Cyno-1 cells can be differentiated in vitro into dopaminergic and serotonergic neurons, contractile cardiomyocyte-like cells, smooth muscle, ciliated epithelia, and adipocytes. When Cyno-1 cells were injected into severe combined immunodeficient mice, teratomas with derivatives from all three embryonic germ layers were obtained. When grown on fibronectin/laminin-coated plates and in neural progenitor medium, Cyno-1 cells assume a neural precursor phenotype (immunoreactive for nestin). However, these cells remain proliferative and express no functional ion channels. When transferred to differentiation conditions, the nestin-positive precursors assume neuronal and epithelial morphologies. Over time, these cells acquire electrophysiological characteristics of functional neurons (appearance of tetrodotoxin-sensitive, voltage-dependent sodium channels). These results suggest that stem cells derived from the parthenogenetically activated nonhuman primate egg provide a potential source for autologous cell therapy in the female and bypass the need for creating a competent embryo

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mentioning

confidence: 99%

Nonhuman primate parthenogenetic stem cells

Vrana

Hipp

Goss

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

176

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“…Even though parthenogenetic embryos die shortly after implantation, their cells are capable of participating in normal development of chimeras when aggregated with fertilized embryos (Fundele et al 1989, Nagy et al 1989, Allen et al 1995, Boediono et al 1999. Parthenogenetic cells were detected in some organs and tissues of adult chimeras.…”

Section: Somatic Cell Nuclear Transfer In Rabbitsmentioning

confidence: 99%

Rabbit somatic cell cloning: effects of donor cell type, histone acetylation status and chimeric embryo complementation

Yang

Hao²,

Keßler³

et al. 2007

Reproduction

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The epigenetic status of a donor nucleus has an important effect on the developmental potential of embryos produced by somatic cell nuclear transfer (SCNT). In this study, we transferred cultured rabbit cumulus cells (RCC) and fetal fibroblasts (RFF) from genetically marked rabbits (Alicia/Basilea) into metaphase II oocytes and analyzed the levels of histone H3-lysine 9-lysine 14 acetylation (acH3K9/14) in donor cells and cloned embryos. We also assessed the correlation between the histone acetylation status of donor cells and cloned embryos and their developmental potential. To test whether alteration of the histone acetylation status affects development of cloned embryos, we treated donor cells with sodium butyrate (NaBu), a histone deacetylase inhibitor. Further, we tried to improve cloning efficiency by chimeric complementation of cloned embryos with blastomeres from in vivo fertilized or parthenogenetic embryos. The levels of acH3K9/14 were higher in RCCs than in RFFs (P!0.05). Although the type of donor cells did not affect development to blastocyst, after transfer into recipients, RCC cloned embryos induced a higher initial pregnancy rate as compared to RFF cloned embryos (40 vs 20%). However, almost all pregnancies with either type of cloned embryos were lost by the middle of gestation and only one fully developed, live RCC-derived rabbit was obtained. Treatment of RFFs with NaBu significantly increased the level of acH3K9/14 and the proportion of nuclear transfer embryos developing to blastocyst (49 vs 33% with non-treated RFF, P!0.05). The distribution of acH3K9/14 in either group of cloned embryos did not resemble that in in vivo fertilized embryos suggesting that reprogramming of this epigenetic mark is aberrant in cloned rabbit embryos and cannot be corrected by treatment of donor cells with NaBu. Aggregation of embryos cloned from NaBu-treated RFFs with blastomeres from in vivo derived embryos improved development to blastocyst, but no cloned offspring were obtained. Two live cloned rabbits were produced from this donor cell type only after aggregation of cloned embryos with a parthenogenetic blastomere. Our study demonstrates that the levels of histone acetylation in donor cells and cloned embryos correlate with their developmental potential and may be a useful epigenetic mark to predict efficiency of SCNT in rabbits.

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“…In the cow, chimaera production has been applied to rescue parthenogenetic embryos by aggregating them with fertilized ones (Boediono et al, 1999). In the sheep embryonic chimaeras were produced between cleaving embryos (Fehilly et al, 1984) and by introducing inner cell masses to blastocysts (Butler et al, 1987).…”

Section: Experimental Chimaerism In Mammalsmentioning

confidence: 99%

Experimental embryonic-somatic chimaerism in the sheep confirmed by random amplified polymorphic DNA assay

Karasiewicz¹,

Sacharczuk²,

Wąs³

et al. 2008

Int. J. Dev. Biol.

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Developmental potencies of sheep somatic cells (foetal fibroblasts, FFs) in chimaeric animals were analysed. FFs from pigmented Polish Heatherhead (wrzosowka) breed were microsurgically injected into morulae or blastocysts of white Polish Merino breed (5 cells to each embryo). In one experiment the cells were stained with vital fluorescent dye PKH26, and chimaeric blastocysts were cultured in vitro to confirm the presence of fluorescent cells. In the majority of experiments the blastocysts were transferred to synchronized recipient ewes for development until term. Cultured embryonic cells (CEC), earlier known to produce chimaeras, were injected into blastocysts in control experiments. Seven young were born from FF-injected embryos and three were born from CEC-injected ones. All of them were white, but all three control lambs and three experimental lambs showed small areas of skin pigmentation, which indicated Heatherhead CEC or FF contribution. Tissue samples originating from three germ layers were taken from two FFsoriginating presumably chimaeric lambs (male and female) at the age of one month for DNA analysis. The random amplified polymorphic DNA-PCR method supplied two markers of chimaerism, which were amplification products of 643 bp and 615 bp long DNA fragments, found in tissues of experimental lambs as well as in FFs, but not in the blood of parents of blastocysts. The 643 bp marker was found in the majority of tissues of both lambs. The 615 bp amplicon was detected in the skin and lungs of the female lamb and in the hooves of the male lamb. Our data show that foetal fibroblasts introduced to sheep blastocysts can participate in development and can contribute to all tissue lineages up to at least one month of age.

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Offspring born from chimeras reconstructed from parthenogenetic and in vitro fertilized bovine embryos

Cited by 38 publications

References 45 publications

Nonhuman primate parthenogenetic stem cells

Nonhuman primate parthenogenetic stem cells

Rabbit somatic cell cloning: effects of donor cell type, histone acetylation status and chimeric embryo complementation

Experimental embryonic-somatic chimaerism in the sheep confirmed by random amplified polymorphic DNA assay

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