Off-the-shelf acellular fetal skin scaffold as a novel alternative to buccal mucosa graft: the development and characterization of human tissue-engineered fetal matrix in rabbit model of hypospadiasis

Sobhani, Soheila; Khaboushan, Alireza Soltani; Jafarnezhad-Ansariha, Fahimeh; Azimzadeh, Ashkan; Payeh, Mozhgan Danesh; Kajbafzadeh, Abdol-Mohammad

doi:10.1007/s11255-022-03249-7

Cited by 2 publications

(2 citation statements)

References 26 publications

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“…The results of the SEM showed that the three-dimensional scaffolds had a honeycomb structure, which is similar to the geometric structure of the three-dimensional cell scaffold prepared after the decellularization of human skin. Such a honeycomb geometry has the conditions for HSFs to grow on it [12]. The HSFs (labeling with amino graphene quantum dots in advance, the marking method is obtained from my previously published paper [13]) and HVECs were inoculated at 1:4 (HVECs:HSFs) on tissue engineering scaffolds as the experimental group.…”

Section: Results Of Hsfs Growth On Tissue Engineering Scaffoldsmentioning

confidence: 99%

Human Vascular Endothelial Cells Promote the Secretion of Vascularization Factors and Migration of Human Skin Fibroblasts under Co-Culture and Its Preliminary Application

Hou

Gao

et al. 2022

IJMS

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The good treatment of skin defects has always been a challenge in the medical field, and the emergence of tissue engineering skin provides a new idea for the treatment of injured skin. However, due to the single seed cells, the tissue engineering skin has the problem of slow vascularization at the premonitory site after implantation into the human body. Cell co-culture technology can better simulate the survival and communication environment of cells in the human body. The study of multicellular co-culture hopes to bring a solution to the problem of tissue engineering. In this paper, human skin fibroblasts (HSFs) and human vascular endothelial cells (HVECs) were co-cultured in Transwell. The Cell Counting Kit 8 (CCK8), Transwell migration chamber, immunofluorescence, Western blot (WB), and real time quantitative PCR (RT-qPCR) were used to study the effects of HVECs on cell activity, migration factor (high mobility group protein 1, HMGB1) and vascularization factor (vascular endothelial growth factor A,VEGFA and fibroblast growth factor 2, FGF2) secretion of HSFs after co-cultured with HVECs in the Transwell. The biological behavior of HSFs co-cultured with HVECs was studied. The experimental results are as follows: (1)The results of cck8 showed that HVECS could promote the activity of HSFs. (2) HVECs could significantly promote the migration of HSFs and promote the secretion of HMGB1. (3) HVECs could promote the secretion of VEGFA and FGF2 of HSFs. (4) The HVECs and HSFs were inoculated on tissue engineering scaffolds at the ratio of 1:4 and were co-cultured and detected for 7 days. The results showed that from the third day, the number of HSFs was significantly higher than that of the control group without HVECs.

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Section: Results Of Hsfs Growth On Tissue Engineering Scaffoldsmentioning

confidence: 99%

Human Vascular Endothelial Cells Promote the Secretion of Vascularization Factors and Migration of Human Skin Fibroblasts under Co-Culture and Its Preliminary Application

Hou

Gao

et al. 2022

IJMS

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“…El-Kassaby et al (83) reported that acellular matrix would be suitable for use in surgical treatment of early strictures with an apparently healthy urethral bed and minimal spongiofibrosis. Primary ADM should not be performed in patients with preoperative infection complicated by urethral stones due to long-term stone irritation and poor local mucosal conditions, which increase the risk of infection or poor healing outcomes (81). In general, ADM represents a novel approach for material selection in urethral repair and reconstruction that offers benefits such as straightforward implementation, convenience, minimal invasiveness, and no additional sampling.…”

Section: Using Cell-free Adm Scaffold Materialsmentioning

confidence: 99%

Acellular dermal matrix in urethral reconstruction

Aodi,

Ying,

Chengyang

et al. 2024

Front. Pediatr.

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The management of severe urethral stricture has always posed a formidable challenge. Traditional approaches such as skin flaps, mucosal grafts, and urethroplasty may not be suitable for lengthy and intricate strictures. In the past two decades, tissue engineering solutions utilizing acellular dermal matrix have emerged as potential alternatives. Acellular dermal matrix (ADM) is a non-immunogenic biological collagen scaffold that has demonstrated its ability to induce layer-by-layer tissue regeneration. The application of ADM in urethral reconstruction through tissue engineering has become a practical endeavor. This article provides an overview of the preparation, characteristics, advantages, and disadvantages of ADM along with its utilization in urethral reconstruction via tissue engineering.

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Off-the-shelf acellular fetal skin scaffold as a novel alternative to buccal mucosa graft: the development and characterization of human tissue-engineered fetal matrix in rabbit model of hypospadiasis

Cited by 2 publications

References 26 publications

Human Vascular Endothelial Cells Promote the Secretion of Vascularization Factors and Migration of Human Skin Fibroblasts under Co-Culture and Its Preliminary Application

Human Vascular Endothelial Cells Promote the Secretion of Vascularization Factors and Migration of Human Skin Fibroblasts under Co-Culture and Its Preliminary Application

Acellular dermal matrix in urethral reconstruction

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