Off-Resonance SERS Nanoprobe-Targeted Screen of Biomarkers for Antigens Recognition of Bladder Normal and Aggressive Cancer Cells

Yang, Yao-Tzu; Hsu, I-Lin; Cheng, Tsung Chieh; Wu, Wen‐Jeng; Lee, Chien‐Wei; Li, Tsung‐Ju; Cheung, Chun In; Chin, Yu‐Cheng; Chen, Hsiao‐Chien; Chiu, Yi‐Chun; Huang, Chih‐Chia; Liao, Mei‐Yi

doi:10.1021/acs.analchem.9b00775

Cited by 33 publications

(38 citation statements)

References 48 publications

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“…Kerr and co-workers [ 40 ] used Raman micro-spectroscopy to differentiate bladder cancer cells from other urine sediments. Yang et al [ 41 ] created a surface enhanced Raman scattering (SERS) assay for specific receptors on cancer cells. The signal amplification properties of SERS allow these molecules to be illuminated in liquid urine specimens.…”

Section: Introductionmentioning

confidence: 99%

Raman chemometric urinalysis (Rametrix) as a screen for bladder cancer

Huttanus

Guruli

et al. 2020

PLoS ONE

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Bladder cancer (BCA) is relatively common and potentially recurrent/progressive disease. It is also costly to detect, treat, and control. Definitive diagnosis is made by examination of urine sediment, imaging, direct visualization (cystoscopy), and invasive biopsy of suspect bladder lesions. There are currently no widely-used BCA-specific biomarker urine screening tests for early BCA or for following patients during/after therapy. Urine metabolomic screening for biomarkers is costly and generally unavailable for clinical use. In response, we developed Raman spectroscopy-based chemometric urinalysis (Rametrix™) as a direct liquid urine screening method for detecting complex molecular signatures in urine associated with BCA and other genitourinary tract pathologies. In particular, the Rametrix TM screen used principal components (PCs) of urine Raman spectra to build discriminant analysis models that indicate the presence/absence of disease. The number of PCs included was varied, and all models were cross-validated by leave-one-out analysis. In Study 1 reported here, we tested the Rametrix™ screen using urine specimens from 56 consented patients from a urology clinic. This proof-of-concept study contained 17 urine specimens with active BCA (BCA-positive), 32 urine specimens from patients with other genitourinary tract pathologies, seven specimens from healthy patients, and the urinalysis control Surine TM. Using a model built with 22 PCs, BCA was detected with 80.4% accuracy, 82.4% sensitivity, 79.5% specificity, 63.6% positive predictive value (PPV), and 91.2% negative predictive value (NPV). Based on the number of PCs included, we found the Rametrix TM screen could be fine-tuned for either high sensitivity or specificity. In other studies reported here, Rametrix TM was also able to differentiate between urine specimens from patients with BCA and other genitourinary pathologies and those obtained from patients with end-stage kidney disease (ESKD). While larger studies are needed to improve Rametrix TM models and demonstrate clinical relevance, this study demonstrates the ability of the Rametrix TM screen to differentiate urine of

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Section: Introductionmentioning

confidence: 99%

Raman chemometric urinalysis (Rametrix) as a screen for bladder cancer

Huttanus

Guruli

et al. 2020

PLoS ONE

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“…MTT assays confirmed the low cytotoxicity of the Au@TNA and Au@TNA@MB NPs. Upon conjugation with folic acid molecules [ 33 , 34 ], Au@TNA/MB exhibited a better selectivity to bind the folate receptor on HeLa cervical cancer cells and T24 bladder cancer cells. Therefore, targeted PDT treatment could produce a large number of reactive oxygen species (ROS) to efficiently damage cancer cells upon 650 nm irradiation, while neither dark toxicity nor a nontoxic PDT effect on the SV-HUC-1 normal bladder cell line was observed.…”

Section: Introductionmentioning

confidence: 99%

Development of Folic Acid-Conjugated and Methylene Blue-Adsorbed Au@TNA Nanoparticles for Enhanced Photodynamic Therapy of Bladder Cancer Cells

et al. 2020

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Photodynamic therapy (PDT) is a promising treatment for malignancy. However, the low molecular solubility of photosensitizers (PSs) with a low accumulation at borderline malignant potential lesions results in the tardy and ineffective management of recurrent urothelial carcinoma. Herein, we used tannic acid (TNA), a green precursor, to reduce HAuCl4 in order to generate Au@TNA core-shell nanoparticles. The photosensitizer methylene blue (MB) was subsequently adsorbed onto the surface of the Au@TNA nanoparticles, leading to the incorporation of a PS within the organic shell of the Au nanoparticle nanosupport, denoted as Au@TNA@MB nanoparticles (NPs). By modifying the surface of the Au@TNA@MB NPs with the ligand folate acid (FA) using NH2-PEG-NH2 as a linker, we demonstrated that the targeted delivery strategy achieved a high accumulation of PSs in cancer cells. The cell viability of T24 cells decreased to 87.1%, 57.1%, and 26.6% upon treatment with 10 ppm[Au] Au@TNA/MB NPs after 45 min, 2 h, and 4 h of incubation, respectively. We also applied the same targeted PDT treatment to normal urothelial SV-HUC-1 cells and observed minor phototoxicity, indicating that this safe photomedicine shows promise for applications aiming to achieve the local depletion of cancer sites without side effects.

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“…At present, the research of SERS substrates focusses on improving the sensitivity, reproducibility, stability, uniformity and flexibility. Great progress has been made on SERS substrates, including various types of materials and nanostructures, as well as flexible and convenient 2D and 3D substrates [ 46 , 47 , 48 , 49 , 50 ]. The AuNP colloid is the most stable and controllable SERS substrate, and the larger and more uniform the particle size, the better and more stable the enhancement effect [ 51 , 52 ].…”

Section: Resultsmentioning

confidence: 99%

Rapid Quantitative Detection of Deltamethrin in Corydalis yanhusuo by SERS Coupled with Multi-Walled Carbon Nanotubes

et al. 2020

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With the increase in demand, artificially planting Chinese medicinal materials (CHMs) has also increased, and the ensuing pesticide residue problems have attracted more and more attention. An optimized quick, easy, cheap, effective, rugged and safe (QuEChERS) method with multi-walled carbon nanotubes as dispersive solid-phase extraction sorbents coupled with surface-enhanced Raman spectroscopy (SERS) was first proposed for the detection of deltamethrin in complex matrix Corydalis yanhusuo. Our results demonstrate that using the optimized QuEChERS method could effectively extract the analyte and reduce background interference from Corydalis. Facile synthesized gold nanoparticles with a large diameter of 75 nm had a strong SERS enhancement for deltamethrin determination. The best prediction model was established with partial least squares regression of the SERS spectra ranges of 545~573 cm−1 and 987~1011 cm−1 with a coefficient of determination (R2) of 0.9306, a detection limit of 0.484 mg/L and a residual predictive deviation of 3.046. In summary, this article provides a new rapid and effective method for the detection of pesticide residues in CHMs.

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Off-Resonance SERS Nanoprobe-Targeted Screen of Biomarkers for Antigens Recognition of Bladder Normal and Aggressive Cancer Cells

Cited by 33 publications

References 48 publications

Raman chemometric urinalysis (Rametrix) as a screen for bladder cancer

Raman chemometric urinalysis (Rametrix) as a screen for bladder cancer

Development of Folic Acid-Conjugated and Methylene Blue-Adsorbed Au@TNA Nanoparticles for Enhanced Photodynamic Therapy of Bladder Cancer Cells

Rapid Quantitative Detection of Deltamethrin in Corydalis yanhusuo by SERS Coupled with Multi-Walled Carbon Nanotubes

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