Oestrus Control and Early Pregnancy Diagnosis in the Swamp Buffalo: Comparison of Enzyme Immunoassay and Radioimmunoassay for Plasma Progesterone

Use of progesterone 11-glucuronide--alkaline phosphatase conjugate in a sensitive microtitre-plate enzymeimmunoassay of progesterone in milk and its application to pregnancy testing in dairy cattle

“…EIAs have been described for the determination of progesterone in extracts of serum or plasma from animals of several species (Dray, Andrieu & Renaud, 1975; Joyce, Read & Fahmy, 1977;Patricot, Poggi & Revol, 1978; Seeger, Thurow, Haede & Knappe, 1979;Kamonpatana et al, 1979;Johmen, Nakao, Tsunoda & Kawata, 1980) and in extracts of whole milk of cows (van de Wiel, Kamonpatana, Ngramsurijaroy, Koops & Singhajan, 1982), defatted milk (Nakao, Sugihashi, Saga, Tsunoda & Kawata, 1983) and milk fat (Arnstadt & Cleere, 1981). Direct EIA procedures requiring no extraction of progesterone have been described for bovine whole milk (Sauer, Foulkes & Cookson, 1981;Chang & Estergreen, 1984) and cream (Nakao & Kawata, 1980).…”

A review of female cattle reproduction with special reference to a comparison between buffaloes, cows and zebu

“…This is a real difference between breeds and is reflected in milk fat and whole milk progesterone measurements (Kamonpatana, Ngramsuriyaroj, Srisakwattana & Chantaraprateep, 1983a).…”

“…Enzyme immunoassays are currently being examined (Kamonpatana et al, 1979b;Arnstadt & Cleere, 1981;Foulkes, Cookson & Sauer, 1982) (Kamonpatana, Parnpai, Ngramsuriyaroj & Srisakwattana, 1983b …”

Identification of progesterone and 17α-hydroxyprogesterone in polychaetes (Perinereis sp.) and the effects of hormone extracts on penaeid oocyte development in vitro

“…Steroid radioimmunoassay was carried out at The Use of Nuclear Technology to Improve Reproductive Efficiency in Dairy Cattle and Swamp Buffalo Project, Research and Development Centre for Livestock Production Technology, Faculty of Veterinary Science, Chulalongkorn, Thailand using the technique developed by Kamonpatana et al (1979). Fifty microliter of reconstituent tissue extracts was taken in an assay tube and 100 μl of anti-progesterone prepared from rabbit and 100 μl of 8000 counts per min (cpm) of (1, 2, 6, 7-H 3 )-progesterone (92 Ci/mmol) (Amersham, UK) were added.…”