Oestrone sulphate measurement in bovine serum during late pregnancy and its relationship with the number of calves born

Worsfold, A.; Williams, Gethin; Williams, D.O.

doi:10.1016/0007-1935(89)90007-9

Cited by 12 publications

(4 citation statements)

References 2 publications

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“…(1983) found that the concentration of E1‐S in milk was influenced by factors like changes in the transport of E1‐S across the mammary gland and in the local synthesis and metabolism of E1‐S. In addition, the plasma or serum concentration of E1‐S reported to be higher in twin than singleton pregnancies in the cow (Worstfold et al. 1989; Dobson et al.…”

Section: Discussionmentioning

confidence: 99%

Serum Profiles of Pregnancy‐Associated Glycoprotein, Oestrone Sulphate and Progesterone During Gestation and Some Factors Influencing the Profiles in Ethiopian Borana and Crossbred Cattle

Lobago

Bekana

Gustafsson

et al. 2009

Reprod Domestic Animals

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This study presents serum concentrations of pregnancy-associated glycoprotein (PAG), oestrone sulphate (E1-S) and progesterone (P4), and the effects of some dam and foetus-related factors on these profiles during gestation in Borana and crossbred cattle. The PAG concentrations at 4th week post-conception ranged from 1.5-5.5 and 2.1-4.7 ng/ml in Borana (n = 6) and crossbred (n = 8) cattle, respectively. The mean PAG concentrations increased progressively from 4th to 33rd week of gestation (from 3.3-173 ng/ml for Borana and 4.2-240 ng/ml for crossbred cattle) and reached peak around calving. Breed, parity status, dam body weight, foetal sex and foetal birth weight significantly influenced the PAG concentrations. After delivery, the PAG concentrations declined steadily to 5.7 ng/ml in Borana (n = 7) and 3.9 ng/ml in crossbred (n = 6) cattle 10 weeks post-partum. The serum E1-S concentrations at 17th week of pregnancy ranged from 0.3-2.6 and 0.9-5.7 ng/ml in Borana (n = 8) and crossbred (n = 9) cattle, respectively. The mean E1-S concentrations increased progressively from 17th to 33rd week of gestation (from 1.1-4.6 ng/ml for Borana and 2.7-10.8 ng/ml for crossbred). Breed, parity status, dam body weight and foetal sex significantly influenced E1-S concentrations. The P4 concentrations at 4th week of pregnancy ranged from 3.2-5.1 and 1.7-8.9 ng/ml in Borana (n = 6) and crossbred (n = 8) cattle, respectively. The P4 level remained elevated throughout pregnancy. This study indicated that the serum PAG and P4 concentrations at 4th and E1-S approximately 17th week of pregnancy were above the cut-off value for pregnancy test and the hormonal profiles observed were comparable to the previous reports. Furthermore, the PAG and E1-S profiles were considerably influenced by factors such as breed, weight and parity status of the dam, and foetal sex and foetal birth weight (only PAG).

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Section: Discussionmentioning

confidence: 99%

Serum Profiles of Pregnancy‐Associated Glycoprotein, Oestrone Sulphate and Progesterone During Gestation and Some Factors Influencing the Profiles in Ethiopian Borana and Crossbred Cattle

Lobago

Bekana

Gustafsson

et al. 2009

Reprod Domestic Animals

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“…It has been reported that the plasma estrone sulfate concentrations were higher in the mid-to late gestation period in twin-bearing cows than in singleton-bearing cows 1,7,20 . However, this difference had not been observed during the first trimester of gestation because of the limited sensitivity of previous assay systems 1,17 .…”

Section: Discussionmentioning

confidence: 94%

“…Therefore, it is considered to be a practical indicator of placental function and has been used in pregnancy diagnosis 9,10 and in predicting the number of fetuses 1,17,20 . The objectives of this study were to characterize the peripheral plasma concentrations of estrone, estradiol-17β and estrone sulfate during the first trimester of gestation in dairy cows using a sensitive assay system, and to correlate the results with the origin of the embryos and fetal number.…”

Section: Jarq 37 (3) 2003mentioning

confidence: 99%

Changes in Plasma Estrogen Concentrations during the First Trimester of Gestation in Dairy Cows: Comparison with the Origin of Embryos and Fetal Number

Hirako

Takahashi

et al. 2003

JARQ

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The objectives of this study were to determine the profiles of plasma estrogen concentrations during the first trimester of gestation and to correlate them with the origin of the embryos and fetal number. Pregnancies were induced either by artificial insemination (AI) on the day of estrus (day 0) or by transfer of 1 or 2 blastocysts produced in vitro (IVF-ET) on day 7. Five singleton-bearing cows impregnated by AI, and 6 singleton and 6 twin pregnancies induced by IVF-ET were used in the experiment. Blood was collected by jugular venipuncture at 2 to 5-day intervals until day 100. The concentrations of plasma estrone, estradiol-17β and estrone sulfate were measured by using a radioimmunoassay. Concentrations of all the estrogens fluctuated at low levels until day 50 in all the groups. Thereafter, the concentration of estrone sulfate gradually increased, whereas those of estrone and estradiol-17β remained at basal levels until day 80. The concentration of estrone sulfate after day 50 was affected by the day of gestation and the number of fetuses. After day 80, the concentration of estrone sulfate increased drastically, coinciding with the increases in the estrone and estradiol-17β concentrations. The rate of increase in the concentration of estrone sulfate during days 80 to 100 was the highest among all the estrogens. In the singleton pregnancies, the concentrations of estrone and estrone sulfate between days 80 and 100 were higher in the artificially inseminated cows than in the IVF-ET cows. In the pregnancies induced by IVF-ET, estrone sulfate levels were higher in the twin-bearing cows than in the singleton-bearing cows during days 50 to 80 and 80 to 100. These results suggest that the concentrations of estrogens in bovine peripheral blood plasma could be applied for monitoring fetoplacental development in the first trimester of gestation.Discipline: Animal industry Additional key words: estradiol-17β, estrone, estrone sulfate, in vitro fertilization and multiple pregnancies JARQ 37 (3), 195 -200 (2003) http://www.jircas.affrc.go.jp Present address:

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“…And those placental estrogens are important factors controlling caruncular growth, differentiation and function [11]. The concentration of E1 sulfate, a conjugated form of E1, is higher in multiple pregnancy cows than in single pregnancy cows [3,25,31]. In addition, a significant influence of the fetal number on the levels of bovine plasma E1 but not E2β in the last two trimesters of gestation, was reported [19].…”

mentioning

confidence: 99%

The Development of a Sensitive Enzymeimmunoassay for the Determination of Estrone and Estradiol-17.BETA. in Bovine Blood Plasma based on the Same Homologous Combination with Antiserum and Steroid-Enzyme Conjugate

Takenouchi

Oshima

Shimada

et al. 2004

The Journal of Veterinary Medical Science

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ABSTRACT. The development of a sensitive enzymeimmunoassay (EIA) for the determination of estrone (E1) and estradiol-17β (E2β) in bovine plasma is described. The assay is a homologous double-antibody EIA with E2β 17hemisuccinate (HS) as hapten for the immunoreactive reagent. The antiserum was raised against E2β 17HS bovine serum albumin conjugate in the rabbit, and E2β 17HS-horseradish peroxidase was used as steroid-enzyme conjugate. Each estrogen EIA was distinguished only by using the each working stan dard and sample for the EIA. Bovine plasma E1 and E2β were extracted and purified before EIA. The antiserum was used at 1:1,750,000 dilutions for EIA. Estrone and E2β showed high cross-reactivity with the antiserum (E1: 350.7%, E2β:100%). The sensitivities were <0.03 pg/well for E1 and <0.12 pg/well for E2 β. Recovery rates of E1 and E2β added to bovine blood plasma were 94.5% and 93.9%, respectively. The precision for EIA of estrogens was below 9.7%. The profiles of either estrogen as determined by EIA corresponded closely well with follicle dynamics in the cow during the estrous cycles and with placental function in pregnant animals. In co nclusion, our new EIA can be applied with sufficient sensitivities, recovery and precision for the routine analysis of E1 and E2β concentrations in bovine plasma. KEY WORDS: cattle, enzymeimmunoassay, estrone, estradiol-17β.

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Oestrone sulphate measurement in bovine serum during late pregnancy and its relationship with the number of calves born

Cited by 12 publications

References 2 publications

Serum Profiles of Pregnancy‐Associated Glycoprotein, Oestrone Sulphate and Progesterone During Gestation and Some Factors Influencing the Profiles in Ethiopian Borana and Crossbred Cattle

Serum Profiles of Pregnancy‐Associated Glycoprotein, Oestrone Sulphate and Progesterone During Gestation and Some Factors Influencing the Profiles in Ethiopian Borana and Crossbred Cattle

Changes in Plasma Estrogen Concentrations during the First Trimester of Gestation in Dairy Cows: Comparison with the Origin of Embryos and Fetal Number

The Development of a Sensitive Enzymeimmunoassay for the Determination of Estrone and Estradiol-17.BETA. in Bovine Blood Plasma based on the Same Homologous Combination with Antiserum and Steroid-Enzyme Conjugate

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