1975
DOI: 10.1111/j.1432-1033.1975.tb02329.x
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Oestrogen Receptors in Chick Oviduct

Abstract: Macromolecular components with properties of oestrogen receptors have been identified in the 0.5 M KCI nuclear soluble, the nuclear insoluble and the cytosol fractions of laying hen and immature (2-4 weeks, untreated by hormone) chicken oviduct.In the 0.5 M KC1 extract of laying hen oviduct nuclei, a receptor, of protein nature according to the cffects of enzymic treatments, has been identified. It exhibits high affinity for oestradiol with an apparent equilibrium association constant K, = 4 . lo9 M-' at 4 "C.… Show more

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Cited by 59 publications
(15 citation statements)
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References 33 publications
(11 reference statements)
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“…The results obtained with these exchange techniques are in reasonable agreement with published data on oestrogen receptor site concentrations in chick oviduct cells [7,. Studies on the ontogeny of oestrogen receptor concentrations in the chiclk Mullerian duct have demonstrated the presence of 590 fmol of specifically bound oestradiol/mg DNA (810 sites/cell) in oviduct nuclei immediately prior to birth, and 22.50 fmol of cytoplasmic receptor/mg DNA (3100 sites/cell) in 4-day-old chickens [15,16].…”
Section: Discussionsupporting
confidence: 81%
“…The results obtained with these exchange techniques are in reasonable agreement with published data on oestrogen receptor site concentrations in chick oviduct cells [7,. Studies on the ontogeny of oestrogen receptor concentrations in the chiclk Mullerian duct have demonstrated the presence of 590 fmol of specifically bound oestradiol/mg DNA (810 sites/cell) in oviduct nuclei immediately prior to birth, and 22.50 fmol of cytoplasmic receptor/mg DNA (3100 sites/cell) in 4-day-old chickens [15,16].…”
Section: Discussionsupporting
confidence: 81%
“…animal hormone receptors (15), but contrasts with others for which dissociation is relatively slow and strongly temperaturedependent (1,5,6,10 (Fig. 4) With the improved binding assay procedure that was used here, the concentration of specific NAA-binding sites, indicated by the point at which the line in a Scatchard plot intersects the abscissa, varied from about 30 to 50 pmol/g of tissue, fresh weight, in a number of different experiments (e.g.…”
Section: Resultsmentioning
confidence: 99%
“…Nuclei from the oviducts of egg-laying hens were prepared according to Best-Belpomme et al (9). Estrogen receptor was extracted from purified nuclei with 500 mM KCl/50 mM Tris HCl, pH 8.7/10 mM EDTA/2 mM 2-mercaptoethanol/1 mM phenylmethylsulfonyl fluoride.…”
Section: Methodsmentioning
confidence: 99%
“…A second chromatographic step on a hydroxylapatite column was necessary to improve the DNA binding capacity of the estrogen-receptor complex. In some experiments estrogen-receptor was first "stripped" of its endogenous estrogen by a 10-min incubation at 370C with 5% charcoal/0.5% dextran (9).…”
Section: Methodsmentioning
confidence: 99%