Oestriolglycuronide

Use of the dietary anoestrous adults rat for the assay of the oestrus‐inducing gonadotrophic substance

“…The criteria of vaginal oestrus laid down by Marrian & Parkes [1929 b] and Cohen, Marrian & Odell [1936] …”

“…With preparations of urinary origin, adequate precautions must therefore be taken to remove last traces of oestrin. During pregnancy more than 99 % of the total oestrogenic material is excreted as the ether-insoluble conjugate with glucuronic acid [Cohen, Marrian & Watson, 1935;. The water-soluble combined oestrogens are present as the sodium salts which are less soluble in aqueous alcohol and acetone than the free acid complexes.…”

“…50 for oestriol glucuronide in castrated mice of the same strain and under the same conditions of testing (2-7 jig. [Cohen, Marrian & Odell, 1936]). No interference from traces of combined oestrogens would thus be expected unless the latter were several hundred times more active in the intact animal.…”

“…In these cases the degree of accuracy achieved is restricted by the extent of the variation in susceptibility of the individual animals and variation in sensitivity with age. Puberty is normally reached at such a variable age that widely divergent states of ovarian development occur [Davy, 1934] when the test is carried out (usually during the fourth week of life). Wide fluctuation in the amount of a hormone required to elicit the same response in different animals would thus be expected.…”

Hormone Excretion in Liver Disease 1

“…It will be noted that, if production is constant, the estrogen level of the blood may presumably be modified through: (a) excretion of the material in the urine and bile (and through the intestinal wall?) some being the subcutaneously injected conjugated estrogens are less "potent" than the free forms (4). However, more recent evidence indicates the role of the human liver in estrogen inactivation is less impressive (5) and that the relative inactivity of conjugated estrogens is not apparent when tested at the target tissue level (6,7).…”