Oestradiol Enhances Plasma Growth Hormone and Insulin‐like Growth Factor‐I Concentrations and Increased the Expression of their Receptors mRNAs in the Liver of Ovariectomized Cows

Çolak, Mehmet; Shimizu, Takashi; Matsunaga, Naofumi; Murayama, Chiaki; Nagashima, S; Kataoka, Motoyuki; Kawashima, Chiho; Matsui, Motozumi; Dorland, HA van; Rm, Bruckmaier; Miyamoto, Akio

doi:10.1111/j.1439-0531.2011.01754.x

Cited by 12 publications

(16 citation statements)

References 63 publications

(116 reference statements)

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“…An attractive candidate in this context could be growth hormone since it has clear actions in the liver and has been shown to be differentially regulated by kisspeptin-10 in the presence of E2-17β or P4 [47] In that study, growth hormone was increased by kisspeptin-10 treatment in ovariectomized cows that were treated with either E2-17β or P4 but growth hormone was not increased by kisspeptin-10 in the absence of E2-17β and P4. In addition, E2-17β has been found to increase circulating growth hormone concentrations and insulin-like growth factor-1, in addition to increasing the mRNA for their receptors in the liver [48].…”

Section: Discussionmentioning

confidence: 99%

Estradiol and Progesterone Exhibit Similar Patterns of Hepatic Gene Expression Regulation in the Bovine Model

et al. 2013

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Female sex steroid hormones, estradiol-17β (E2-17β) and progesterone (P4) regulate reproductive function and gene expression in a broad range of tissues. Given the central role of the liver in regulating homeostasis including steroid hormone metabolism, we sought to understand how E2-17β and P4 interact to affect global gene expression in liver. Ovariectomized cows (n = 8) were randomly assigned to 4 treatment groups applied in a replicated Latin Square design: 1) No hormone supplementation, 2) E2-17β treatment (ear implant), 3) P4 treatment (intravaginal inserts), and 4) E2-17β combined with P4. After 14 d of treatment, liver biopsies were collected, allowing 28 d intervals between periods. Changes in gene expression in the liver biopsies were monitored using bovine-specific arrays. Treatment with E2-17β altered expression of 479 genes, P4 472 genes, and combined treatment significantly altered expression of 468 genes. In total, 578 genes exhibited altered expression including a remarkable number (346 genes) that responded similarly to E2-17β, P4, or combined treatment. Additional evidence for similar gene expression actions of E2-17ß and/or P4 were: principal component analysis placed almost every treatment array at a substantial distance from controls; Venn diagrams indicated overall treatment effects for most regulated genes; clustering analysis indicated the two major clusters had all treatments up-regulating (172 genes) or down-regulating (173 genes) expression. Thus, unexpectedly, common biological pathways were regulated by E2-17β and/or P4 in liver. This indicates that the mechanism of action of these steroid hormones in the liver might be either indirect or might occur through non-genomic pathways. This unusual pattern of gene expression in response to steroid hormones is consistent with the idea that there are classical and non-classical tissue-specific responses to steroid hormone actions. Future studies are needed to elucidate putative mechanism(s) responsible for overlapping actions of E2-17β and P4 on the liver transcriptome.

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Section: Discussionmentioning

confidence: 99%

Estradiol and Progesterone Exhibit Similar Patterns of Hepatic Gene Expression Regulation in the Bovine Model

et al. 2013

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“…To examine the expression of growth hormone (GH) receptor (GHR), insulin receptor (INSR), and β‐actin mRNA, the muscle ( n = 11) and liver ( n = 8) of male calves were sampled on a single occasion between 8 and 10 days of age via percutaneous needle biopsy (Bard Monopty Disposable Core Biopsy Instrument., 16G × 9 cm; Bard Peripheral Vascular, Inc.) under local anesthesia (Lidocaine hydrochloride; Xylocaine ® Jelly 2%, AstraZeneca, Sweden) after intravenous administration of xylazine (Ceractal ® , 2% solution; Bayer, Osaka, Japan) and 5% tranexamic acid (Vasolamin Injection; Meiji Seika Pharma, Tokyo, Japan), and stored at −80°C until analyzed. Total RNA from homogenized tissue samples was extracted using TRIZOL ® regent, as described previously (Çolak et al, ). Real‐time PCR quantification of GHR, INSR, and β‐actin gene expression was performed in an iQ5 Cycler (Bio‐Rad Laboratories) using a commercial kit (QuantiTect™ SYBR ® Green PCR; Qiagen GmbH) as described previously (Çolak et al, ).…”

Section: Methodsmentioning

confidence: 99%

“…Total RNA from homogenized tissue samples was extracted using TRIZOL ® regent, as described previously (Çolak et al, ). Real‐time PCR quantification of GHR, INSR, and β‐actin gene expression was performed in an iQ5 Cycler (Bio‐Rad Laboratories) using a commercial kit (QuantiTect™ SYBR ® Green PCR; Qiagen GmbH) as described previously (Çolak et al, ). The primers used for GH, INSR, and β‐actin amplification were those described previously (Çolak et al, , Table ).…”

Section: Methodsmentioning

confidence: 99%

“…Real‐time PCR quantification of GHR, INSR, and β‐actin gene expression was performed in an iQ5 Cycler (Bio‐Rad Laboratories) using a commercial kit (QuantiTect™ SYBR ® Green PCR; Qiagen GmbH) as described previously (Çolak et al, ). The primers used for GH, INSR, and β‐actin amplification were those described previously (Çolak et al, , Table ). GH and INSR gene expression values were normalized using β‐actin as the internal standard.…”

Section: Methodsmentioning

confidence: 99%

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Insulin resistance: Relationship between indices during late gestation in dairy cows and effects on newborn metabolism

Hasegawa

Iwase

Takagi

et al. 2019

Animal Science Journal

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To investigate the relationship between insulin resistance indices [“Revised quantitative insulin sensitivity check index” (RQUICKI; RQ), “Revised quantitative insulin sensitivity check index ‐ β‐hydroxybutyrate” (RQUICKIBHB; RQBHB), and “Homeostasis model assessment‐insulin resistance” (HOMA‐IR; HR)], and metabolic parameters in dams during late gestation, and their newborn calves. Blood was sampled twice weekly during the experimental period in 30 dry Holstein cows. In calves, blood sampling and body weight measurements were performed immediately after birth, and in 1‐week‐old male calves, liver and muscle biopsy samples were obtained for determining metabolic factor mRNA levels. RQ and RQBHB were negatively correlated with insulin, nonesterified fatty acid, BHB, and albumin and were positively correlated with leptin levels in blood during late gestation (p < .05). RQ, rather than RQBHB, reflected metabolism of dams, while stronger positive correlations were present between HR and blood insulin concentrations than other parameters, and calves of dams with high HR had low body weight, and high liver and muscle expression of growth hormone and insulin receptor mRNA (p < .05). RQ and HR of dams during late gestation could serve as indicators of dam metabolism and predictors of metabolism in newborn calves respectively.

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“…Circulating IGF‐1 concentrations are higher during the follicular phase than during the luteal phase in adult cows (Kawashima et al., ), and oestrogen receptors have been detected in the bovine pituitary (Connor et al., ) and the liver (Pfaffl et al., ). Moreover, in adult ovariectomized cows, oestradiol injection was found to increase plasma IGF‐1 concentration (Colak et al., ). Therefore, oestradiol injections in pre‐pubertal heifers should also affect IGF‐1 concentration, and the resultant changes may be useful as an index of IGF‐1 productive capacity.…”

Section: Introductionmentioning

confidence: 99%

Polymorphism in promoter region of growth hormone receptor is associated with potential production capacity of insulin‐like growth factor‐1 in pre‐pubertal Holstein heifers

Kawashima

Munakata

Matsui

et al. 2016

Animal Physiology Nutrition

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Insulin-like growth factor-1 (IGF-1) is one of the important factors for growth, milk production and reproductive functions and mainly released from the liver in response to growth hormone (GH) via GH receptor (GHR) in cattle. Recently, some single nucleotide polymorphisms (SNPs) were identified in the bovine GHR gene. Some GHR-SNPs were shown to be related to plasma IGF-1 concentration in cattle. Hence, the capacity to IGF-1 production in the liver might be affected by GHR-SNP and associated with performance in the future. This study examined whether GHR-SNP is associated with IGF-1 production in the liver of pre-pubertal heifers. In 71 Holstein calves, blood samples for genomic DNA extraction were obtained immediately after birth. To genotype the GHR-SNPs in the promoter region, polymerase chain reaction (PCR) products were digested with restriction enzyme NsiI (cutting sites: AA, AG and GG). All heifers at 4 months of age were intramuscularly injected with 0.4 mg oestradiol benzoate. Blood samples were obtained from the jugular vein just before (0 h) and 24 h after injection. The number of AA, AG and GG at the NsiI site was 0, 17 and 54 respectively. In AG and GG, plasma GH concentrations were higher pre-injection than 24 h post-injection (p < 0.01). Moreover, plasma GH concentrations in AG post-injection were higher than in GG (p < 0.05). In contrast, the GG genotype exhibited higher plasma IGF-1 concentrations in pre-injection than post-injection (p < 0.01), although oestradiol did not change IGF-1 concentration in the AG genotype. We conclude that the GG polymorphism in the promoter region of GHR is associated with a higher potential capacity of IGF-1 production in the liver of cattle.

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Oestradiol Enhances Plasma Growth Hormone and Insulin‐like Growth Factor‐I Concentrations and Increased the Expression of their Receptors mRNAs in the Liver of Ovariectomized Cows

Cited by 12 publications

References 63 publications

Estradiol and Progesterone Exhibit Similar Patterns of Hepatic Gene Expression Regulation in the Bovine Model

Estradiol and Progesterone Exhibit Similar Patterns of Hepatic Gene Expression Regulation in the Bovine Model

Insulin resistance: Relationship between indices during late gestation in dairy cows and effects on newborn metabolism

Polymorphism in promoter region of growth hormone receptor is associated with potential production capacity of insulin‐like growth factor‐1 in pre‐pubertal Holstein heifers

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