Oestradiol and progesterone receptors in the pig oviduct during the oestrous cycle

Stanchev, Ph.; Rodriguez‐Martinez, Heriberto; Edqvist, L.‐E.; Eriksson, Håkan

doi:10.1016/0022-4731(85)90150-5

Cited by 28 publications

(11 citation statements)

References 36 publications

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“…However, synthesis and secretion of proteins that are actually inhibited by E 2 could be stimulated as a result of down-regulation of E 2 receptors. Studies in the hen [31], mouse [32], pig [33], and primates [34] indicate that absolute and relative amounts of receptors for E 2 and P 4 vary in the oviduct during the estrous cycle and pregnancy. Moreover, oviductal functions such as velocity of egg transport [32] and oviductal epithelial cell proliferation [34] also change in response to manipulations of the steroid environment and steroid binding to their receptors.…”

Section: Discussionmentioning

confidence: 97%

Persistent Dominant Follicle Alters Pattern of Oviductal Secretory Proteins from Cows at Estrus1

Binelli

Hampton

Buhi

et al. 1999

Biology of Reproduction

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The experimental objective was to compare synthesis of oviductal secretory proteins of dairy cows bearing a persistent dominant follicle (PDF) versus a fresh dominant follicle (FDF) at estrus. On Day 7 after synchronized estrus (Day 0), cows received an intravaginal progesterone device and injection of prostaglandin F2alpha (PGF2alpha). On Day 9, cows received an injection of a GnRH agonist (FDF group; n = 3) or received no injection (PDF group, n = 3). On Day 16, all cows received PGF2alpha, and progesterone devices were removed. At slaughter on Day 18 or Day 19, oviducts ipsilateral and contralateral to the dominant follicle were divided into infundibulum, ampulla, and isthmus regions. Explants from oviductal regions were cultured in minimal essential medium supplemented with [3H]leucine for 24 h. Two-dimensional fluorographs of proteins in conditioned media were analyzed by densitometry. Rate of incorporation of [3H]leucine into macromolecules was greater in the infundibulum, ampulla, and isthmus of FDF cows (p < 0.01). Overall, intensities of radiolabeled secretory protein (P) 2 and P13 were greater for FDF than for PDF. In the ampulla, P14 was more intense for FDF while P7 was more intense for PDF. Abundance of P1 in the isthmus was greater for PDF cows. Across regions, P5, P6, P8, P9, and P11 were more intense for PDF than for FDF in the ipsilateral side. In the contralateral side, P19 was more intense for PDF than for FDF, whereas P6, P8, P9, and P11 were more intense for FDF. Differences in biosynthetic activity and in secreted oviductal proteins from cows bearing a PDF may contribute to the decrease in fertility associated with a PDF.

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Section: Discussionmentioning

confidence: 97%

Persistent Dominant Follicle Alters Pattern of Oviductal Secretory Proteins from Cows at Estrus1

Binelli

Hampton

Buhi

et al. 1999

Biology of Reproduction

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“…Oestrogen appears to drive ciliogenesis since ciliated cells are especially numerous in the pig oviduct during oestrous (Nayak et al 1976a;Wu et al 1976). Additionally, maximal NC cell differentiation occurs during the follicular phase, when large concentrations of circulating oestrogens are present (Nayak et al 1976b;Stanchev et al 1985). The cytoplasm of NC cells contains characteristic secretory granules of variable size (Teixeira and Haddad 1988).…”

Section: Introductionmentioning

confidence: 98%

“…In the pig, the surface morphology of the oviduct epithelium has been investigated by scanning electron microscopy (Stalheim et al 1975;Wu et al 1976;Abe and Oikawa 1992) and results show that there are regional and cyclic changes in the morphological features of the POEC. It is well established for many species, including the pig, that these changes are correlated with varying oestrogen (E2) and progesterone (P) blood levels (Stanchev et al 1985;Brenner et al 1990; Abe and Oikawa 1993). The effects of E2 and P are mediated in a direct or indirect manner through specific nuclear receptors, but the exact hormonal regulation in the oviduct needs further research.…”

Section: Introductionmentioning

confidence: 98%

Immunophenotype of porcine oviduct epithelial cells during the oestrous cycle: a double-labelling immunohistochemical study

Šteffl

Schweiger

Amselgruber

2004

Histochemistry and Cell Biology

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The luminal epithelium of the porcine oviduct is composed of ciliated cells and secretory cells, but it is assumed for several species that under the control of steroid hormones secretory cells are able to be transformed into ciliated cells. In order to better understand such physiological changes during the different stages of the oestrous cycle, we evaluated epithelial cell proliferation together with oestrogen receptor alpha (ERalpha) expression of porcine ampullary oviducts. To identify the immunophenotype of proliferating cells, double immunohistochemistry was performed using anti-chromogranin A antibody (anti-CgA) as the second primary antibody. Anti-CgA, recently shown to be an immunocytochemical marker of ciliated cells of the cow, also labelled specifically the luminal surface of ciliated cells of the pig. Double labelling of sections with the monoclonal antibody MIB-1 against the proliferation-associated nuclear epitope Ki-67 and anti-CgA clearly demonstrates that MIB-1 was selectively localised in the nuclei of secretory cells. Proliferative activity was not observed in CgA-positive ciliated cells in all examined stages of the oestrous cycle. The percentage of Ki-67-positive epithelial cells was higher at pro-oestrus, compared with the other stages of the oestrous cycle. Furthermore, ERalpha immunoreactivity was exclusively detected in the nuclei of the epithelial cells, which were negative for CgA. We conclude, therefore, that oestrogen may induce the initial proliferation of secretory cells and promote the differentiation into ciliated cells.

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“…In pigs, most of the studies on ER in the reproductive organs have been done in gilts (Pack et al. 1978; Stanchev et al. 1985, 1990; Saunders et al.…”

Section: Introductionmentioning

confidence: 99%

“…In a study using oestrogen receptor knockout mice, it was shown that ERa is the major subtype regarding regulation of uterine activities (Couse and Korach 1999;Muramatsu and Inoue, 2000). In pigs, most of the studies on ER in the reproductive organs have been done in gilts (Pack et al 1978;Stanchev et al 1985Stanchev et al , 1990Saunders et al 1989;Geisert et al 1993;Tarleton et al 1998). In sows, having experienced pregnancies, it has earlier been shown that the uterine ER concentration (binding assay) was about three times higher compared with cycling young gilts (Koziorowski et al 1984).…”

Section: Introductionmentioning

confidence: 99%

Immunohistochemical Studies on Oestrogen Receptor Alpha (ERα) and the Proliferative Marker Ki‐67 in the Sow Uterus at Different Stages of the Oestrous Cycle

Sukjumlong

Kaeoket

Dalin

et al. 2003

Reprod Domestic Animals

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In order to better understand physiological changes during the different stages of the oestrous cycle, immunohistochemistry was used in the present study to investigate the distribution of oestrogen receptor alpha (ER alpha) as well as the proliferative marker Ki-67, in the sow uterus during the oestrous cycle. Uterine samples were collected from multiparous sows with normal reproductive performance at selected stages of the oestrous cycle: at late dioestrus (d 17), prooestrus (d 19), oestrous (d 1), early dioestrus (d 4) and dioestrus (d 11-12), respectively. The tissue samples were fixed in 10% formaldehyde, embedded in paraffin and subjected to immunohistochemistry using monoclonal antibodies against ER alpha (C-311) and Ki-67 (MM-1). In general, the immunostaining of both ER alpha and Ki-67 was confined to nuclei of the target cells. Variations were seen, not only at the different stages of the oestrous cycle, but also in the different tissue compartments of the uterus. In the epithelia, the strongest ER alpha staining and highest amount of positive Ki-67 cells were found at early dioestrus. In the myometrium, the highest levels of staining of both ER alpha and Ki-67 positive cells were found at pro-oestrus and oestrus. For the proliferative marker, Ki-67, no positive cells were found at dioestrus and late dioestrus in the epithelium and myometrium. In the connective tissue stroma (subepithelial layer), the highest number of ER alpha positive cells were found at oestrus, which was significantly different compared with other stages (p< or = 0.05), whereas the levels of Ki-67 positive cells were relatively low and did not differ between the stages examined. Significant correlations between the number of ER alpha positive cells in the stroma and Ki-67 positive cells in the epithelia were observed. This suggests indirect regulatory mechanisms on epithelial proliferation via ER alpha in the stroma. In conclusion, these findings in the sow uterus show that the presence of ER alpha as well as Ki-67 protein varies not only between different stages of the oestrous cycle but also between different tissue compartments of the uterus. These findings indicate various regulatory mechanisms and stress the importance of localising ER alpha and proliferating cells in different uterine tissues.

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Oestradiol and progesterone receptors in the pig oviduct during the oestrous cycle

Cited by 28 publications

References 36 publications

Persistent Dominant Follicle Alters Pattern of Oviductal Secretory Proteins from Cows at Estrus1

Persistent Dominant Follicle Alters Pattern of Oviductal Secretory Proteins from Cows at Estrus1

Immunophenotype of porcine oviduct epithelial cells during the oestrous cycle: a double-labelling immunohistochemical study

Immunohistochemical Studies on Oestrogen Receptor Alpha (ERα) and the Proliferative Marker Ki‐67 in the Sow Uterus at Different Stages of the Oestrous Cycle

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