Buffalo is an important worldwide species in terms of milk and meat production as well as draft. The major constraints to full exploitation of the productive potential of buffalo are its inherent low reproductive efficiency due to delayed puberty, higher age at first calving, long post partum anoestrus period, long intercalving period, silent heat coupled with poor expression of oestrus, seasonality in breeding and low conception rate. Reproductive cycles in buffalo are regulated by endocrine-neuroendocrine interactions between hypothalamic, gonadotropic, gonadal and other hormones. To improve the buffalo reproductive efficiency, the investigation on endocrine aspects is useful to gain a better knowledge of buffalo reproduction. This review is focused on the information available on various basic hormones (Melatonin, Progesterone (P4), Estradiol 17B (E2), Follicle stimulating hormone (FSH), Luteinizing hormone (LH)) and on new hormones (Inhibin, Glycoproteins associated with pregnancy (PAGs), Leptin) related to reproductive function.