OECD Detailed Review Paper (DRP) number 31 on “Cell Transformation Assays for Detection of Chemical Carcinogens”: main results and conclusions

Vasseur, Paule; Lasne, C

doi:10.1016/j.mrgentox.2011.11.007

Cited by 59 publications

(35 citation statements)

References 13 publications

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“…In accordance, it could be noted that in vitro cell transformation assays have been proposed as alternatives to longterm animal studies. In fact, OECD has specific guidelines on ''Cell transformation assays for detection of chemical carcinogens'' (Vasseur and Lasne 2012) with accumulated evidence that the cellular and molecular processes involved in vitro cell transformation are similar to those of in vivo carcinogenesis (Creton et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Acute and long-term in vitro effects of zinc oxide nanoparticles

et al. 2015

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Since most of the toxic studies of zinc oxide nanoparticles (ZnO NPs) focused on acute and high-dose exposure conditions, the aim of the present study was to fill the existing knowledge gap of long-term effects of ZnO NPs at sub-toxic doses. To overcome this point, we have evaluated the toxic, genotoxic, and carcinogenic effects of ZnO NPs under long-term treatments (12 weeks), using a sub-toxic dose (1 µg/mL) according to acute 48-h exposure. Preliminarily, oxidative stress and genotoxic/oxidative DNA damage were determined under acute exposure and high-dose conditions. To determine the role of oxidative DNA damage, a wild-type mouse embryonic fibroblast (MEF Ogg1 (+/+)) and its isogenic 8-oxo-guanine DNA glycosylase 1 (Ogg1) knockout partner (MEF Ogg1 (-/-)) cell lines were used. Although short-term exposure (24-h) experiments demonstrated that ZnO NPs were able to induce ROS, genotoxicity, and oxidative DNA damage in both cell lines, no effects were obtained under long-term exposure scenario. Thus, 1 µg/mL exposure over 12 weeks was unable to induce genotoxicity as well as cellular transformation in both cell types, as indicated by the lack of observed morphological cell changes, variations in the secretion of matrix metalloproteinases, and anchorage-independent cell growth ability, regarded as cancer-like phenotypic hallmarks. Our results indicate that short-term effects of ZnO NP exposure are not replicated under long-term and sub-toxic dose conditions. All together, the lack of genotoxic/carcinogenic effects after chronic treatments seem to indicate a reduced risk associated with ZnO NP exposure.

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Section: Introductionmentioning

confidence: 99%

Acute and long-term in vitro effects of zinc oxide nanoparticles

et al. 2015

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“…However, their use is limited to the characterization of carcinogenetic risk without a quantitative output. Detailed in vitro carcinogenicity test guidelines have been issued by OECD (Vasseur et al, 2012) and EURL/ ECVAM recommendations include the need to develop human cell assays, encourage studies on tumor promotion mechanisms and develop automation systems for assay scoring (EURL ECVAM 2013; Urani et al, 2009).…”

Section: Meeting Reportmentioning

confidence: 99%

From cells to QSAR: Alternative predictive models in toxicology

Sambuy¹,

Bassi

Scanarotti

et al. 2017

ALTEX

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“…The Detailed Review Paper (DRP) number 31 of the Organization for Economic Cooperation and Development (OECD, 2007) analysed the performance of three models used in Cell Transformation Assays (CTAs) to screen the carcinogenic potential of chemicals: the Syrian hamster embryo (SHE) cells, and the mouse cell lines BALB/c 3T3 and C3H/10T 1 / 2 (OECD, 2007; Vasseur and Lasne, 2012). The report compared CTA results collected from public literature to results from recent genotoxicity tests using mammalian and non-mammalian cell systems.…”

Section: In Vitro Transformation Assays Dunkel Et Al (1981) Comparedmentioning

confidence: 99%