Odorant‐binding protein (<scp>OBP</scp>) genes affect host specificity in a fig–pollinator mutualistic system

Wang, N.; Wang, N. X.; Niu, Lihua; Bian, Sheng‐Nan; Xiao, Jin‐Hua; Huang, Dawei

doi:10.1111/imb.12110

Cited by 24 publications

(22 citation statements)

References 42 publications

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“…A sensitive chemosensory system is essential for insects to accomplish many important physiological behaviors, such as the detection of food, predators, hosts, oviposition sites, and mates 1 , 2 . Many proteins have been found to be involved in odorant reception in the antennae, the major chemosensory organ.…”

Section: Introductionmentioning

confidence: 99%

Structure-Based Analysis of the Ligand-Binding Mechanism for DhelOBP21, a C-minus Odorant Binding Protein, from Dastarcus helophoroides (Fairmaire; Coleoptera: Bothrideridae)

Li¹,

Yu²,

Yi³

et al. 2015

Int. J. Biol. Sci.

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Odorant binding proteins (OBPs) transport hydrophobic odor molecules across the sensillar lymph to trigger a neuronal response. Herein, the Minus-C OBP (DhelOBP21) was characterized from Dastarcus helophoroides, the most important natural parasitic enemy insect that targets Monochamus alternatus. Homology modeling and molecular docking were conducted on the interaction between DhelOBP21 and 17 volatile molecules (including volatiles from pine bark, the larva of M. alternatus, and the faeces of the larva). The predicted three-dimensional structure showed only two disulfide bridges and a hydrophobic binding cavity with a short C-terminus. Ligand-binding experiments using N-phenylnaphthylamine (1-NPN) as a fluorescent probe showed that DhelOBP21 exhibited better binding affinities against those ligands with a molecular volume between 100 and 125 Å³ compared with ligands with a molecular volume between 160 and 185 Å³. Molecules that are too big or too small are not conducive for binding. We mutated the amino acid residues of the binding cavity to increase either hydrophobicity or hydrophilia. Ligand-binding experiments and cyber molecular docking assays indicated that hydrophobic interactions are more significant than hydrogen-bonding interactions. Although hydrogen-bond interactions could be predicted for some binding complexes, the hydrophobic interactions had more influence on binding following hydrophobic changes that affected the cavity. The orientation of ligands affects binding by influencing hydrophobic interactions. The binding process is controlled by multiple factors. This study provides a basis to explore the ligand-binding mechanisms of Minus-C OBP.

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Section: Introductionmentioning

confidence: 99%

Structure-Based Analysis of the Ligand-Binding Mechanism for DhelOBP21, a C-minus Odorant Binding Protein, from Dastarcus helophoroides (Fairmaire; Coleoptera: Bothrideridae)

Li¹,

Yu²,

Yi³

et al. 2015

Int. J. Biol. Sci.

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“…A sensitive chemosensory system is essential for insects to achieve a series of complex behavioural responses such as detecting food, avoiding predators, host localization, mating, and seeking oviposition sites (de Bruyne and Baker, 2008;Wang et al, 2014). Antennae, which house masses of sensilla, are the main olfactory organs in insects.…”

Section: Introductionmentioning

confidence: 99%

Chemosensory proteins used as target for screening behaviourally active compounds in the rice pest Cnaphalocrocis medinalis (Lepidoptera: Pyralidae)

Duan

Wang

2018

Insect Molecular Biology

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Reverse chemical ecology based on insect functional odorant binding proteins has been extensively studied to screen behaviourally active compounds, whereas chemosensory proteins (CSPs), which are reportedly involved in olfactory chemical reception and could serve as molecular targets remain unclear. In the present study, two behaviourally active compounds for Cnaphalocrocis medinalis, a serious pest of rice in Asia, were successfully screened via an antenna-biased CSP, CmedCSP33. Fluorescence competitive binding assays showed that CmedCSP33 could bind to seven out of 32 rice volatiles. Fluorescence quenching experiments revealed that CmedCSP33 forms a stable complex with nerolidol and β-ionone, and circular dichroism (CD) spectra demonstrated that these two compounds cause conformational changes in CmedCSP33. Furthermore, H-tube olfactometer bioassays showed that C. medinalis displayed prominent attractant responses to nerolidol and prominent repellent responses to β-ionone. Additionally, binding assays and CD spectra at different pH values implied that extensive conformational changes may be a general feature of CSPs for triggering the subsequent chemical transduction. Overall, our findings provide evidence for the involvement of CSPs in olfactory perception, and a protocol for effectively screening behaviourally active compounds.

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“…Five GRs and forty-six OR genes were found and may be associated with the process of host specialization (Xiao et al, 2013). Ceratosolen solmsi marchali, the expression of two OBP genes (CsolOBP4 and CsolOBP5) was further confirmed using real-time quantitative PCR (Wang et al, 2014). In order to better understand the attraction mechanism of fig wasp to the VOCs, we need genomic or transcriptome studies on more fig wasps species and to identify more genes related to their chemoreception.…”

mentioning

confidence: 99%

Transcriptome analysis of genes involved in the response of a pollinator fig wasp to volatile organic compounds from its host figs

Zeng

Kjellberg

2018

Acta Oecologica

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Odorant‐binding protein (OBP) genes affect host specificity in a fig–pollinator mutualistic system

Cited by 24 publications

References 42 publications

Structure-Based Analysis of the Ligand-Binding Mechanism for DhelOBP21, a C-minus Odorant Binding Protein, from Dastarcus helophoroides (Fairmaire; Coleoptera: Bothrideridae)

Structure-Based Analysis of the Ligand-Binding Mechanism for DhelOBP21, a C-minus Odorant Binding Protein, from Dastarcus helophoroides (Fairmaire; Coleoptera: Bothrideridae)

Chemosensory proteins used as target for screening behaviourally active compounds in the rice pest Cnaphalocrocis medinalis (Lepidoptera: Pyralidae)

Transcriptome analysis of genes involved in the response of a pollinator fig wasp to volatile organic compounds from its host figs

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