Odor maps of aldehydes and esters revealed by functional MRI in the glomerular layer of the mouse olfactory bulb

Xu, Fuqiang; Liu, Nian; Kida, Ikuhiro; Rothman, Douglas L.; Hyder, Fahmeed; Shepherd, Gordon M.

doi:10.1073/pnas.1832864100

Cited by 178 publications

(168 citation statements)

References 57 publications

Supporting

Mentioning

152

Contrasting

Unclassified

Order By: Relevance

“…Imaging studies using various activity markers have shown that odor compounds evoke spatially organized, combinatorial patterns of neural activity in OBs (Mombaerts et al, 1996;Friedrich and Korsching, 1998;Johnson et al, 1998;Rubin and Katz, 1999;Belluscio and Katz, 2001;Xu et al, 2003; and ALs (Joerges et al, 1997;Carlsson et al, 2002;Hansson et al, 2003). In most of these cases, however, the cell types imaged were unknown, although in others (Zhao et al, 1998;Araneda et al, 2000;Ma and Shepherd, 2000;Gaillard et al, 2002;Wachowiak and Cohen, 2003) primary-afferent input was imaged.…”

Section: Discussionmentioning

confidence: 99%

Chemosensory Selectivity of Output Neurons Innervating an Identified, Sexually Isomorphic Olfactory Glomerulus

Reisenman¹,

Christensen²,

Hildebrand³

2005

J. Neurosci.

View full text Add to dashboard Cite

The antennal lobe (AL) of insects, like the olfactory bulb of vertebrates, is characterized by discrete modules of synaptic neuropil called glomeruli. In some insects (e.g., moths and cockroaches), a few glomeruli are sexually dimorphic and function in labeled lines for processing of sensory information about sex pheromones. Controversy still exists, however, about whether projection (output) neurons (PNs) of glomeruli in the main AL are also narrowly tuned. We examined this critical issue in the AL of the moth Manduca sexta. We used intracellular recording and staining techniques to investigate the chemosensory tuning of PNs innervating an identifiable, sexually isomorphic glomerulus, G35, in the main AL. We found that the morphological features and chemosensory tuning of G35-PNs were nearly identical in females and males. G35-PNs responded to low concentrations of the plant-derived volatile compound cis-3-hexenyl acetate (c3HA), but the sensitivity threshold of female PNs was lower than that of male PNs. The propionate and butyrate homologs of c3HA could evoke excitatory responses but only at moderate-to-high concentrations. Other plant volatiles did not evoke responses from G35-PNs. Moreover, PNs innervating glomeruli near G35 (in females) showed little or no response to c3HA. Female G35-PNs were hyperpolarized by (Ϯ)linalool, a compound that excites PNs in an adjacent glomerulus, thus providing evidence for lateral-inhibitory interactions between glomeruli. Our results show that PNs arborizing in an identified glomerulus in the main olfactory pathway are morphologically and physiologically equivalent in both sexes and have characteristic, limited molecular receptive ranges that are highly conserved across individuals.

show abstract

Section: Discussionmentioning

confidence: 99%

Chemosensory Selectivity of Output Neurons Innervating an Identified, Sexually Isomorphic Olfactory Glomerulus

Reisenman¹,

Christensen²,

Hildebrand³

2005

J. Neurosci.

View full text Add to dashboard Cite

show abstract

“…The resolution of functional magnetic resonance imaging (fMRI) shows constant progress. Recently, fMRI odour maps (Xu et al, 2003, Schafer et al, 2006 have been recorded in the mouse and rat, opening the possibility of a totally non-invasive glomeruli mapping on the entire olfactory bulb. We have presented here a detection method designed for a 2D image, but clearly it would also be valid for a volume.…”

Section: Assessing the Significance Of Spatial Detailsmentioning

confidence: 99%

Wavelet-based multi-resolution statistics for optical imaging signals: Application to automated detection of odour activated glomeruli in the mouse olfactory bulb

Bathellier¹,

Ville²,

Blu³

et al. 2007

NeuroImage

View full text Add to dashboard Cite

“…They have been studied in considerable detail (Shepherd et al, 2004). These studies include anatomical characteristics Powell, 1971, 1972;Mori and Yoshihara, 1995;Lodovichi et al, 2003), pioneering work and additional studies on regional electrical characteristics of mitral cells (Bischofberger and Jonas, 1997;Christie and Westbrook, 2003;Debarbieux et al, 2003), synaptic interactions with sensory neurons and local neurons of the olfactory bulb (Nicoll, 1971;Nowycky et al, 1981;Carlson et al, 2000;Urban and Sakmann, 2002), and properties of mitral cell responses to electrical and odorant stimulation (Freeman, 1974;Harrison and Scott, 1986;Hamilton and Kauer, 1989;Charpak et al, 2001;Margrie et al, 2001;Wachowiak and Cohen, 2001;Cang and Isaacson, 2003;Margrie and Schaefer, 2003;Xu et al, 2003). The results of these studies show that mitral cells are complex operational units.…”

Section: Introductionmentioning

confidence: 99%

Voltage Imaging from Dendrites of Mitral Cells: EPSP Attenuation and Spike Trigger Zones

Djurišić¹,

Antic²,

Chen³

et al. 2004

J. Neurosci.

118

130

View full text Add to dashboard Cite

To obtain a more complete description of individual neurons, it is necessary to complement the electrical patch pipette measurements with technologies that permit a massive parallel recording from many sites on neuronal processes. This can be achieved by using voltage imaging with intracellular dyes. With this approach, we investigated the functional structure of a mitral cell, the principal output neuron in the rat olfactory bulb. The most significant finding concerns the characteristics of EPSPs at the synaptic sites and surprisingly small attenuation along the trunk of the primary dendrite. Also, the experiments were performed to determine the number, location, and stability of spike trigger zones, the excitability of terminal dendritic branches, and the pattern and nature of spike initiation and propagation in the primary and secondary dendrites. The results show that optical data can be used to deduce the amplitude and shape of the EPSPs evoked by olfactory nerve stimulation at the site of origin (glomerular tuft) and to determine its attenuation along the entire length of the primary dendrite. This attenuation corresponds to an unusually large mean apparent "length constant" of the primary dendrite. Furthermore, the images of spike trigger zones showed that an action potential can be initiated in three different compartments of the mitral cell: the soma-axon region, the primary dendrite trunk, and the terminal dendritic tuft, which appears to be fully excitable. Finally, secondary dendrites clearly support the active propagation of action potentials.

show abstract

Odor maps of aldehydes and esters revealed by functional MRI in the glomerular layer of the mouse olfactory bulb

Abstract: authors note that Fig. 1 a and c, the third and fourth panels of Fig. 3a, and the first panel of Fig. 3b were printed incorrectly due to a printer's error. The corrected figures and their legends appear below.

Cited by 178 publications

References 57 publications

Chemosensory Selectivity of Output Neurons Innervating an Identified, Sexually Isomorphic Olfactory Glomerulus

Chemosensory Selectivity of Output Neurons Innervating an Identified, Sexually Isomorphic Olfactory Glomerulus

Wavelet-based multi-resolution statistics for optical imaging signals: Application to automated detection of odour activated glomeruli in the mouse olfactory bulb

Voltage Imaging from Dendrites of Mitral Cells: EPSP Attenuation and Spike Trigger Zones

Contact Info

Product

Resources

About