Odontoglossum Ringspot Virus

Edwardson, J. R.; Zettler, F. W.

doi:10.1007/978-1-4684-7026-0_12

Cited by 6 publications

(3 citation statements)

References 43 publications

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“…Odontoglossum ringspot virus (ORSV) is a member of the Tobamovirus genus of positive-strand RNA viruses [10]. There are 12 definite and 2 possible viruses which may be placed within the genus [ 14].…”

Section: Introductionmentioning

confidence: 99%

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The complete nucleotide sequence and genome organization of odontoglossum ringspot tobamovirus RNA

Ryu

Park

1995

Archives of Virology

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The complete nucleotide sequence of the genomic RNA of odontoglossum ringspot tobamovirus (ORSV) was determined. The RNA genome of ORSV is 6618 nucleotides long and contains five open reading frames (ORFs 1 to 5) coding for proteins of M(r) 126 K, 181 K, 34 K, 18 K and 52 K, respectively. This is the longest RNA of the known viruses of the Tobamovirus genus. The sequences of the ORSV RNA encoded proteins exhibit high homology to the proteins of the members of the Tobamovirus genus. The genomic organization and sequence analysis showed that ORSV is more closely related to tobacco mild green mosaic virus (TMGMV), pepper mild mottle virus (PMMV), tomato mosaic virus (ToMV) and TMV than to cucumber green mottle mosaic virus (CGMMV) and sunn-hemp mosaic virus (SHMV).

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Section: Introductionmentioning

confidence: 99%

“…ORSV was first isolated from Rossioglossum grande, formerly called Odontoglossum grande [18,21]. It has been found in most of the regions of the world where orchids are cultivated [10,43]. The natural host range of ORSV is known to be limited to monocotyledonous Orchidaceae plants.…”

Section: Introductionmentioning

confidence: 99%

The complete nucleotide sequence and genome organization of odontoglossum ringspot tobamovirus RNA

Ryu

Park

1995

Archives of Virology

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“…CyMV usually causes chlorosis and necrosis of leaf and floral parts in many different orchid genera (Faccioli and Marani 1979, Yuan et al 1979). Like CyMV, ORSV has been reported to be associated with many cases of blossom necrosis and flower colour break in different orchid genera (Edwardson and Zettler 1986). Large scale surveys have shown that all cultivated Oncidium plants were infected by CyMV and to a lesser extend by ORSV (Chia et al 1991).…”

Section: Introductionmentioning

confidence: 99%

Growth and photosynthesis of virus‐infected and virus‐eradicated orchid plants exposed to different growth irradiances under natural tropical conditions

Ouyang

Chia

2004

Physiologia Plantarum

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Responses of virus‐infected (VI) and virus‐eradicated (VE) Oncidium Gower Ramsey orchid plants grown under 30% of prevailing solar radiation and those transferred from 30 to 60% and 100% of prevailing solar radiation were studied under natural tropical conditions. Plants grown under 30% of prevailing solar radiation suffered lower leaf and floral production and reduced photosynthesis. When the irradiance was increased to 60% of prevailing solar radiation, enhancement of leaf and floral production and photosynthetic capacities were achieved. However, when the plants were transferred from 30 to 100% of prevailing solar radiation, the growth and photosynthetic capacities of the plants were significantly reduced. All plants exhibited a midday depression in photosynthetic CO2 assimilation (A), stomatal conductance (gs) and Fv/Fm ratio. The degree of midday depression of these parameters was not only associated with high temperatures and high irradiances but also with virus infection. Midday Fv/Fm ratio depression indicated that dynamic photo‐inhibition occurred in all plants grown under all three light conditions. However, chronic photo‐inhibition, measured by pre‐dawn Fv/Fm ratio and chlorophyll content, occurred only in those plants transferred from 30 to 100% of prevailing solar radiation. Hence, it is concluded that the VI Oncidium Gower Ramsey was more susceptible to high irradiance than the VE plants.

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A DNA clone encoding the full-length infectious genome of odontoglossum ringspot tobamovirus and mutagenesis of its coat protein gene

Wong

1998

Arch. Virol.

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A full-length DNA clone encoding the genome of odontoglossum ringspot tobamovirus (ORSV) was synthesized and placed adjacent to a bacteriophage T7 RNA polymerase promoter. Capped-RNA transcripts produced in vitro were highly infectious when mechanically inoculated onto seedlings of Nicotiana benthamiana and Oncidium Gower Ramsey. A representative clone, designated pOT2, caused a disease phenotype identical to that produced by parental viral RNA. ELISA, Western blot analysis, Northern blot hybridization and electron microscopy verified the infectivity of pOT2. A coat protein deficient mutant of the clone, pO delta CP1, was produced with the initiation codon of the coat protein cistron of ORSV abolished. Transcripts from pO delta CP1 were infective, able to move in N. benthamiana but produced no coat protein. This demonstrates that the coat protein was dispensable for RNA replication and for movement. This is believed to be the first report of an ORSV infectious clone driven by a T7 RNA polymerase promoter.

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Odontoglossum Ringspot Virus

Cited by 6 publications

References 43 publications

The complete nucleotide sequence and genome organization of odontoglossum ringspot tobamovirus RNA

The complete nucleotide sequence and genome organization of odontoglossum ringspot tobamovirus RNA

Growth and photosynthesis of virus‐infected and virus‐eradicated orchid plants exposed to different growth irradiances under natural tropical conditions

A DNA clone encoding the full-length infectious genome of odontoglossum ringspot tobamovirus and mutagenesis of its coat protein gene

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