Odontogenic capability: bone marrow stromal stem cells versus dental pulp stem cells

Yu, Jinhua; Wang, Yijing; Deng, Zhihong; Tang, Liang; Li, Yuanfei; Shi, Junnan; Jin, Yan

doi:10.1042/bc20070013

Cited by 189 publications

(157 citation statements)

References 72 publications

(130 reference statements)

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“…6,7,30 We have revealed that uniaxial mechanical stretch stimulated the phosphorylation of ERK and Akt as well as p38 MAPK in DPSCs, suggesting that stretch affects multiple cell functions of DPSCs. In addition, we have first demonstrated that stretch increased the proliferation of DPSCs via ERK activation, while inhibiting the osteogenic differentiation.…”

Section: Discussionmentioning

confidence: 99%

Mechanical Stretch Increases the Proliferation While Inhibiting the Osteogenic Differentiation in Dental Pulp Stem Cells

Hara

Naruse

Ozawa

et al. 2013

Tissue Engineering Part A

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Dental pulp stem cells (DPSCs), which can differentiate into several types of cells, are subjected to mechanical stress by jaw movement and occlusal forces. In this study, we evaluated how the uniaxial mechanical stretch influences proliferation and differentiation of DPSCs. DPSCs were isolated and cultured from male SpragueDawley rats. Cultured DPSCs were identified by surface markers and the differentiation capabilities as adipocytes or osteoblasts. To examine the response to mechanical stress, uniaxial stretch was exposed to cultured DPSCs. We evaluated the impact of stretch on the intracellular signaling, proliferation, osteogenic differentiation, and gene expressions of DPSCs. Stretch increased the phosphorylation of Akt, ERK1/2, and p38 MAP kinase as well as the proliferation of DPSCs. The stretch-induced proliferation of DPSCs was abolished by the inhibition of the ERK pathway. On the other hand, stretch significantly decreased the osteogenic differentiation of DPSCs, but did not affect the adipogenic differentiation. We also confirmed mRNA expressions of osteocalcin and osteopontin were significantly suppressed by stretch. In conclusion, uniaxial stretch increased the proliferation of DPSCs, while suppressing osteogenic differentiation. These results suggest a crucial role of mechanical stretch in the preservation of DPSCs in dentin. Furthermore, mechanical stretch may be a useful tool for increasing the quantity of DPSCs in vitro for regenerative medicine.

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Section: Discussionmentioning

confidence: 99%

Mechanical Stretch Increases the Proliferation While Inhibiting the Osteogenic Differentiation in Dental Pulp Stem Cells

Hara

Naruse

Ozawa

et al. 2013

Tissue Engineering Part A

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“…BMSC share numerous characteristics with DPSC and are both able to form bone-like or tooth-like structures. However, BMSC display a lower odontogenic potential than DPSC (Yu et al, 2007), indicating that MSC from different embryonic origins are not equivalent. Indeed, DPSC derive from neural crest cells, whereas BMSC originate from the mesoderm.…”

Section: Periodontal Ligament Stem Cells (Pdlsc)mentioning

confidence: 99%

Stem cells for tooth engineering

Bluteau

Luder

Bari³

et al. 2008

eCM

166

152

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Tooth development results from sequential and reciprocal interactions between the oral epithelium and the underlying neural crest-derived mesenchyme. The generation of dental structures and/or entire teeth in the laboratory depends upon the manipulation of stem cells and requires a synergy of all cellular and molecular events that finally lead to the formation of tooth-specific hard tissues, dentin and enamel. Although mesenchymal stem cells from different origins have been extensively studied in their capacity to form dentin in vitro, information is not yet available concerning the use of epithelial stem cells. The odontogenic potential resides in the oral epithelium and thus epithelial stem cells are necessary for both the initiation of tooth formation and enamel matrix production. This review focuses on the different sources of stem cells that have been used for making teeth in vitro and their relative efficiency. Embryonic, post-natal or even adult stem cells were assessed and proved to possess an enormous regenerative potential, but their application in dental practice is still problematic and limited due to various parameters that are not yet under control such as the high risk of rejection, cell behaviour, long tooth eruption period, appropriate crown morphology and suitable colour. Nevertheless, the development of biological approaches for dental reconstruction using stem cells is promising and remains one of the greatest challenges in the dental field for the years to come.

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“…Cells with regenerative capacity and a suitable phenotype for dental tissue engineering have been derived from the apical pulp (Abe et al, 2008), dental pulp (Prescott et al, 2008), cranial neural crest (Jiang et al, 2008), periodontal ligament (Ballini et al, 2007), bone marrow (Hu et al, 2006), dental follicle (Yao et al, 2008), and cells surrounding the vasculature (Murray and Garcia-Godoy, 2004). There appears to be no consensus regarding a preferred cell source for tooth regeneration; however, differences in odontogenic capacity between stem cells derived from the dental pulp and those derived from the bone marrow have been noted (Yu et al, 2007). Odontoblasts and ectomesenchymal cells are difficult to obtain in the clinical setting (Yen and Sharpe, 2008).…”

Section: Cell Sources For Dental Tissue Regenerationmentioning

confidence: 99%

Applications of Microscale Technologies for Regenerative Dentistry

Hacking

Khademhosseini

2009

J Dent Res

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While widespread advances in tissue engineering have occurred over the past decade, many challenges remain in the context of tissue engineering and regeneration of the tooth. For example, although tooth development is the result of repeated temporal and spatial interactions between cells of ectoderm and mesoderm origin, most current tooth engineering systems cannot recreate such developmental processes. In this regard, microscale approaches that spatially pattern and support the development of different cell types in close proximity can be used to regulate the cellular microenvironment and, as such, are promising approaches for tooth development. Microscale technologies also present alternatives to conventional tissue engineering approaches in terms of scaffolds and the ability to direct stem cells. Furthermore, microscale techniques can be used to miniaturize many in vitro techniques and to facilitate high-throughput experimentation. In this review, we discuss the emerging microscale technologies for the in vitro evaluation of dental cells, dental tissue engineering, and tooth regeneration. Abbreviations: AS, adult stem cell; BMP, bone morphogenic protein; ECM, extracellular matrix; ES, embryonic stem cell; HA, hydroxyapatite; FGF-2, fibroblast growth factor; iPS, inducible pleuripotent stem cell; IGF-1, insulin-like growth factor; PDGF, platelet-derived growth factor; PDMS, poly(dimethylsiloxane); PGA, polyglycolate; PGS, polyglycerol sebacate; PLGA, poly-L-lactate-co-glycolate; PLL, poly-L-lactate; RGD, Arg-Gly-Asp attachment site; TCP, tricalcium phosphate; TGF-β, transforming growth factor beta; and VEGF, vascular endothelial growth factor.

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Odontogenic capability: bone marrow stromal stem cells versus dental pulp stem cells

Cited by 189 publications

References 72 publications

Mechanical Stretch Increases the Proliferation While Inhibiting the Osteogenic Differentiation in Dental Pulp Stem Cells

Mechanical Stretch Increases the Proliferation While Inhibiting the Osteogenic Differentiation in Dental Pulp Stem Cells

Stem cells for tooth engineering

Applications of Microscale Technologies for Regenerative Dentistry

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