Odd paired transcriptional activation of decapentaplegic in the Drosophila eye/antennal disc is cell autonomous but indirect

Sen, Aditya; Stultz, Brian G.; Lee, Heuijung; Hursh, Deborah A.

doi:10.1016/j.ydbio.2010.04.003

Cited by 20 publications

(24 citation statements)

References 47 publications

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“…Second, a single mutation in the zinc finger domain of ZIC2 that results in loss of DNA-binding ability (27) exhibited the same repressor activity as normal ZIC2 while still binding to TCF4. These data are in agreement with previous observations in Drosophila where the sequence-specific DNAbinding ability of Opa was not required for its transcriptional activation (33). In addition, our ChIP results clearly confirmed that both overexpressed ZIC2 and TCF4 were localized at the TCF4 target sequence of the AXIN2 promoter, a ubiquitous Wnt target.…”

Section: Function Of Zic2 As a Repressor Of The ␤-Catenin⅐tcf4supporting

confidence: 93%

Transcription Factor Zic2 Inhibits Wnt/β-Catenin Protein Signaling

Pourebrahim

Houtmeyers

Ghogomu

et al. 2011

Journal of Biological Chemistry

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The Zic transcription factors play critical roles during embryonic development. Mutations in the ZIC2 gene are associated with human holoprosencephaly, but the etiology is still unclear. Here, we report a novel function for ZIC2 as a regulator of ␤-catenin⅐TCF4-mediated transcription. We show that ZIC2 can bind directly to the DNA-binding high mobility group box of TCF4 via its zinc finger domain and inhibit the transcriptional activity of the ␤-catenin⅐TCF4 complex. However, the binding of TCF4 to DNA was not affected by ZIC2. Zic2 RNA injection completely inhibited ␤-catenin-induced axis duplication in Xenopus embryos and strongly blocked the ability of ␤-catenin to induce expression of known Wnt targets in animal caps. Moreover, Zic2 knockdown in transgenic Xenopus Wnt reporter embryos led to ectopic Wnt signaling activity mainly at the midbrain-hindbrain boundary. Together, our results demonstrate a previously unknown role for ZIC2 as a transcriptional regulator of the ␤-catenin⅐TCF4 complex.

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Section: Function Of Zic2 As a Repressor Of The ␤-Catenin⅐tcf4supporting

confidence: 93%

Transcription Factor Zic2 Inhibits Wnt/β-Catenin Protein Signaling

Pourebrahim

Houtmeyers

Ghogomu

et al. 2011

Journal of Biological Chemistry

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“…In addition, we identified another Opa BS similar to the Opa consensus motif previously reported by [23] and therefore referred to as SELEX_OpaBS (GCGGGGATG) (Fig 5A and 5B). Employing the phastCons database, which identifies evolutionarily conserved elements in a multiple alignment, to analyze this sequence, we found that both binding sites are conserved among Drosophila species (Fig 5B; conservation score in green ; Opa BS highlighted in yellow ) [24, 25].…”

Section: Resultssupporting

confidence: 65%

The Zic family homologue Odd-paired regulates Alk expression in Drosophila

et al. 2017

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The Anaplastic Lymphoma Kinase (Alk) receptor tyrosine kinase (RTK) plays a critical role in the specification of founder cells (FCs) in the Drosophila visceral mesoderm (VM) during embryogenesis. Reporter gene and CRISPR/Cas9 deletion analysis reveals enhancer regions in and upstream of the Alk locus that influence tissue-specific expression in the amnioserosa (AS), the VM and the epidermis. By performing high throughput yeast one-hybrid screens (Y1H) with a library of Drosophila transcription factors (TFs) we identify Odd-paired (Opa), the Drosophila homologue of the vertebrate Zic family of TFs, as a novel regulator of embryonic Alk expression. Further characterization identifies evolutionarily conserved Opa-binding cis-regulatory motifs in one of the Alk associated enhancer elements. Employing Alk reporter lines as well as CRISPR/Cas9-mediated removal of regulatory elements in the Alk locus, we show modulation of Alk expression by Opa in the embryonic AS, epidermis and VM. In addition, we identify enhancer elements that integrate input from additional TFs, such as Binou (Bin) and Bagpipe (Bap), to regulate VM expression of Alk in a combinatorial manner. Taken together, our data show that the Opa zinc finger TF is a novel regulator of embryonic Alk expression.

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“…preferentially bind BLACK and BLUE chromatin to approximately the same degree ( Fig. 1A; Levine and Davidson 2005;Sen et al 2010). Finally, a set of developmental TRFs including Kni, Tll, Hth, Gro, Tin, Kr, and Cad (Sauer et al 1996;Levine and Davidson 2005;Ciglar and Furlong 2009;Mann et al 2009) preferentially binds BLUE chromatin over all other types (Fig.…”

Section: Chromatin Types Targeted By Transcriptional Regulatorsmentioning

confidence: 89%

Diverse patterns of genomic targeting by transcriptional regulators in Drosophila melanogaster

Slattery

Spokony

et al. 2014

Genome Res.

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Annotation of regulatory elements and identification of the transcription-related factors (TRFs) targeting these elements are key steps in understanding how cells interpret their genetic blueprint and their environment during development, and how that process goes awry in the case of disease. One goal of the modENCODE (model organism ENCyclopedia of DNA Elements) Project is to survey a diverse sampling of TRFs, both DNA-binding and non-DNA-binding factors, to provide a framework for the subsequent study of the mechanisms by which transcriptional regulators target the genome. Here we provide an updated map of the Drosophila melanogaster regulatory genome based on the location of 84 TRFs at various stages of development. This regulatory map reveals a variety of genomic targeting patterns, including factors with strong preferences toward proximal promoter binding, factors that target intergenic and intronic DNA, and factors with distinct chromatin state preferences. The data also highlight the stringency of the Polycomb regulatory network, and show association of the Trithorax-like (Trl) protein with hotspots of DNA binding throughout development. Furthermore, the data identify more than 5800 instances in which TRFs target DNA regions with demonstrated enhancer activity. Regions of high TRF co-occupancy are more likely to be associated with open enhancers used across cell types, while lower TRF occupancy regions are associated with complex enhancers that are also regulated at the epigenetic level. Together these data serve as a resource for the research community in the continued effort to dissect transcriptional regulatory mechanisms directing Drosophila development.

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Odd paired transcriptional activation of decapentaplegic in the Drosophila eye/antennal disc is cell autonomous but indirect

Cited by 20 publications

References 47 publications

Transcription Factor Zic2 Inhibits Wnt/β-Catenin Protein Signaling

Transcription Factor Zic2 Inhibits Wnt/β-Catenin Protein Signaling

The Zic family homologue Odd-paired regulates Alk expression in Drosophila

Diverse patterns of genomic targeting by transcriptional regulators in Drosophila melanogaster

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