Odd chain fatty acid metabolism in mice after a high fat diet

Ampong, Isaac; Ikwuobe, Ogwu John; Brown, James E.; Bailey, Clifford J.; Gao, Dan; Gutierrez-Merino, Jorge; Griffiths, Helen R.

doi:10.1016/j.biocel.2021.106135

Cited by 10 publications

(9 citation statements)

References 33 publications

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“…In several epidemiological studies, C15:0 and C17:0 have been shown to inversely correlate with NAFLD and other metabolic diseases, Pfeuffer & Jaudszus (2016); Mika et al (2016); Yoo et al (2017) . As demonstrated in our previous study that high dietary fat (HFD) intake reduces serum and liver OCFA, OCFA- producing gut microbiota and is associated with impaired liver lipid metabolism Ampong et al (2021) . Methionine adenosylated product, S -adenosylmethionine is a known substrate for methylation in epigenetic and epigenomic pathways, Robinson et al (2016) , and methylation has been linked to NAFLD directly and indirectly via PPARg.…”

Section: Discussionmentioning

confidence: 84%

“…However, in recent times, OCFA have been suggested as important targets to improve metabolic diseases in part due to their inverse association with several cardiometabolic events. In addition to the gut microbiota and diet suggested as potential source of OCFA, several metabolic pathways including phytanoyl-CoA α-hydroxylase (PHYH) , 2-hydroxyphytanoyl-CoA lyase (HACL1) ; pristanal dehydrogenase, branched-chain alpha-keto acid dehydrogenase (BCKDHA) are known to be involved in their synthesis Ampong et al (2021) .…”

Section: Introductionmentioning

confidence: 99%

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Regulation of odd chain fatty acid metabolism in the development of metabolic diseases in mice fed a low protein diet

Ampong

2022

Preprint

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Nonalcoholic fatty liver disease (NAFLD) and Metabolic syndrome (MS) have become a global health concern as incidence of these metabolic disorders is growing rapidly in developing countries particularly in the Middle East, South America and Africa. Studies have shown that protein restriction is associated with increased risk of metabolic diseases, possibly through effects on fatty acid (FA) metabolism. In the present study, we investigated whether a low protein diet modulates FA metabolism and whether methyl donor supplementation can ameliorate these effects and improve metabolic health. Male C57BL/6 mice were fed either a low protein diet (LPD, 90 g/kg protein, n=8), a LPD supplemented with methyl donors (MD-LPD; choline chloride, betaine, methionine, folic acid, vitamin B12, n=8) or normal protein diet (NPD, 180 g/kg protein, n=8) for 7 weeks prior to analysis of serum fatty acid profiles by GC FID and MS and liver fatty acid synthesis and uptake gene expression by RT-qPCR. We observed significant depletion of serum C15:0 and C17:0 in LPD-fed males compared to NPD. Serum long chain saturated FAs C18:0 and C24:0 were increased in LPD male mice compared to NPD. Gene expression analysis revealed an upregulation of hepatic cluster of differentiation 36 (CD36) expression in LPD mice compared to NPD suggesting increased fat uptake in the liver. However, when LPD diet was supplemented with methyl donors, we observed either no change in serum C15: 0 and an increased serum C17:0 compared to LPD with no methyl donor supplementation. Again, methyl donor supplementation upregulated fatty acid desaturase 1 (FADS1), thioredoxin-1 (TRX1) and catalase (CAT) expression in the liver of MD-LPD fed mice compared to LPD mice. Altogether, our study revealed that odd chain fatty acids (OCFA)s are key early markers observed in a suboptimal diet-induced metabolic changes and may be potential targets to improve metabolic health outcomes.

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Section: Discussionmentioning

confidence: 84%

Section: Introductionmentioning

confidence: 99%

Regulation of odd chain fatty acid metabolism in the development of metabolic diseases in mice fed a low protein diet

Ampong

2022

Preprint

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“…In mammals, synthesis of fatty acids by the fatty acid synthase complex occurs in two carbon units meaning that only even acyl chains are produced by de novo lipid synthesis, whereas odd chain fatty acids such as C15 and C17 carbon chains typically come from dietary origins or are produced by the gut microbiota. In mice on HFD, C15 and C17 fatty acids are decreased and correlated with lower abundance of several gut microbial species including Lactobacillus, Bifidobacterium, and Akkermansia that are known to produce these odd chain fatty acids [ 43 ]. However, other studies have shown that in humans C15 and C17 lipids are independently derived from dietary intake or α-oxidation in the persoxisome, respectively [ 44 ].…”

Section: Discussionmentioning

confidence: 99%

Lipid signatures of chronic pain in female adolescents with and without obesity

et al. 2022

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Background Chronic pain in adolescence is associated with diminished outcomes, lower socioeconomic status in later life, and decreased family well-being. Approximately one third of adolescents with chronic pain have obesity compared to the general population. In obesity, lipid signals regulate insulin sensitivity, satiety, and pain sensation. We determined whether there is a distinct lipid signature associated with chronic pain and its co-occurrence with obesity in adolescents. Methods We performed global lipidomics in serum samples from female adolescents (N = 67, 13–17 years old) with no pain/healthy weight (Controls), chronic pain/healthy weight (Pain Non-obese), no pain/obesity (Obese), or chronic pain/obesity (Pain Obese). Results The Pain Non-obese group had lipid profiles similar to the Obese and Pain Obese groups. The major difference in these lipids included decreased lysophosphatidylinositol (LPI), lysophosphatidylcholine (LPC), and lysophosphatidylethanolamine (LPE) in the three clinical groups compared to the Control group. Furthermore, ceramides and sphingomyelin were higher in the groups with obesity when compared to the groups with healthy weight, while plasmalogens were elevated in the Pain Obese group only. Conclusions Serum lipid markers are associated with chronic pain and suggest that specific lipid metabolites may be a signaling mechanism for inflammation associated with co-occurring chronic pain and obesity.

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“…Odd chain saturated fatty acids are produced by α-oxidation in peroxisomes, de novo lipogenesis, from the diet and by gut microbiota. Although present at low concentrations, epidemiological studies show that higher circulating levels of odd chain saturated fatty acids (FA: C15:0 and C17:0) are associated with lower risks of metabolic diseases [ 21 ].…”

Section: Discussionmentioning

confidence: 99%

Lipidomics for Determining Giant Panda Responses in Serum and Feces Following Exposure to Different Amount of Bamboo Shoot Consumption: A First Step towards Lipidomic Atlas of Bamboo, Giant Panda Serum and Feces by Means of GC-MS and UHPLC-HRMS/MS

Zhu

Pan

Guo

et al. 2022

IJMS

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Lipidic metabolites play essential roles in host physiological health and growth performance, serving as the major structural and signaling components of membranes, energy storage molecules, and steroid hormones. Bamboo, as wild giant pandas’ exclusive diet, is the main determinant of giant pandas’ lipidome, both as a direct source and through microbiota activity. Interestingly, the consumption of bamboo has attracted little attention from a lipidomic perspective. In the current study, we outline the lipidomic atlas of different parts of bamboo. By gas chromatography—mass spectrometry (GC-MS), we have been able to obtain the absolute quantification of 35 fatty acids pertaining to short chain fatty acids (8), medium chain fatty acids (6), long chain fatty acids (17), and very long chain fatty acids (4), while liquid chromatography coupled to high-resolution mass spectrometry (UHPLC-HRMS/MS) allowed us to obtain the relative quantification of another 1638 lipids. Among the fatty acids quantified in absolute terms, eight showed significantly distinct concentrations among different bamboo parts. Subsequently, we investigated how the giant panda’s serum and fecal lipidome adapt to the most important annual change in their diet, represented by the consumption of high amounts of bamboo shoots, typical of spring, the weight-gaining season. Five fatty acids were significantly altered in feces and two in serum, respectively, due to the different levels of bamboo shoot consumption. Furthermore, significant differences of the main bacteria strains were observed in feces between the two groups at the genus level, pertaining to Streptococcus, Leuconostoc, and Vagococcus. Correlations between giant panda fecal microbiome and lipidome were evaluated by Pearson correlation analysis. These findings suggest that a balanced diet, important for the overall lipidomic function and giant panda health, could be reached even in this remarkable case of a single food-based diet, by administering to the giant panda’s combinations of different parts of bamboo, with specific lipidome profiles.

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Odd chain fatty acid metabolism in mice after a high fat diet

Cited by 10 publications

References 33 publications

Regulation of odd chain fatty acid metabolism in the development of metabolic diseases in mice fed a low protein diet

Regulation of odd chain fatty acid metabolism in the development of metabolic diseases in mice fed a low protein diet

Lipid signatures of chronic pain in female adolescents with and without obesity

Lipidomics for Determining Giant Panda Responses in Serum and Feces Following Exposure to Different Amount of Bamboo Shoot Consumption: A First Step towards Lipidomic Atlas of Bamboo, Giant Panda Serum and Feces by Means of GC-MS and UHPLC-HRMS/MS

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