ODC-Mediated Biosynthesis and DAO-Mediated Catabolism of Putrescine Involved in the Induction of Rooting by Leaf Explants of Chrysanthemum morifolium Ramat In Vitro

Martin-Tanguy, J.; Aribaud, Marcel; Carré, Monique; Gaspar, Thomas

doi:10.1007/978-1-4615-5403-5_26

Cited by 6 publications

(4 citation statements)

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“…DFMO treatment affected rooting percentage in the presence of auxin, suggesting that polyamines were involved in the rooting process as observed by Kevers et al (1997), Martin-Tanguy et al (1997), and Tonon et al (2001).…”

Section: Discussionmentioning

confidence: 55%

Involvement of polyamines in the adventitious rooting of micropropagated shoots of the apple rootstock MM106

Naija

Elloumi²,

Ammar

et al. 2008

In Vitro Cell.Dev.Biol.-Plant

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Apple rootstock MM106 shoots, raised in vitro, rooted at 96.7% after culture on a medium supplemented with an auxin for 5 d in darkness followed by culture on a second medium without growth regulators for 25 d in light. In control conditions (in absence of auxin in the first medium), these shoots did not root. Putrescine (PUT), spermidine (SPD), cyclohexylamine (CHA), and aminoguanidine (AG) enhanced rooting when applied during the first d of culture in the absence of IBA; on the contrary, α-difluoromethylornithine (DFMO) added to the first medium with IBA inhibited rooting. The endogenous levels of indole 3-acetic acid (IAA) and indole 3-acetylaspartic acid (IAAsp) increased up to a maximum concentration at days 2 and 3, respectively, in initial rooting conditions. PUT, when added with IBA, did not affect the typical IAA and IAAsp increase; when applied alone, it provoked an increase of their levels. Similar results were recorded with CHA. SPD, AG, and DFMO did not induce an increase of IAA and IAAsp in nonrooting conditions. The levels of endogenous PUT increased to a maximum at day 2 in rooting conditions; it was slightly affected by exogenous PUT and CHA application but reduced by SPD, AG, and DFMO. In rooting conditions, if the first medium was supplemented with SPD or AG, a small increase in peroxidase activity was observed, similar to that obtained with PUT treatment. The present work indicates an involvement of polyamines in the control of rooting and an interaction with auxins during the physiological phase of rooting. The consequence of this relationship was a different rooting expression, according especially to the content of these regulators in the culture medium.

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Section: Discussionmentioning

confidence: 55%

Involvement of polyamines in the adventitious rooting of micropropagated shoots of the apple rootstock MM106

Naija

Elloumi²,

Ammar

et al. 2008

In Vitro Cell.Dev.Biol.-Plant

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“…For blanks, TCA was added at zero time. One milliliter of toluene extract was placed in a scintillation solution in glass vials and the radioactivity was measured in a Beckmann LS 1801 scintillator counter (Martin-Tanguy et al 1997).…”

Section: Determination Of Adc Odc and Dao Activitiesmentioning

confidence: 99%

Polyamines and heavy metal stress: the antioxidant behavior of spermine in cadmium- and copper-treated wheat leaves

2006

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Polyamine metabolism, as well as spermine (Spm) antioxidant properties, were studied in wheat leaves under Cd2+ or Cu2+ stress. The oxidative damage produced by both metals was evidenced by an increased of thiobarbituric acid reactive substances (TBARS) and a significant decrease in glutathione under both metal treatments. Ascorbate peroxidase (APOX) and glutathione reductase (GR) activities were reduced by both metals to values ranging from 30% to 64% of the control values. Conversely, copper produced a raise in superoxide dismutase activity. The high putrescine (Put) content detected under Cd2+ stress (282% over the control) was induced by the increased activity of both enzymes involved in Put biosynthesis, arginine decarboxylase (ADC) and ornithine decarboxylase (ODC). However, only ODC activity was increased in wheat leaves subjected to Cu2+ stress, leading to a lower Put rise (89% over the controls). Spermidine (Spd) content was not affected by metal treatments, while Spm was significantly reduced. Pretreatment with Spm completely reverted the metals-induced TBARS increase whereas metals-dependent H2O2 deposition on leaf segments (revealed using diaminobenzidine), was considerably reduced in Spm pretreated leaf segments. This polyamine failed to reverse the depletion in APOX activity and glutathione (GSH) content produced by Cd2+ and Cu2+, although it showed an efficient antioxidant behavior in the restoration of GR activity to control values. These results suggest that Spm could be exerting a certain antioxidant function by protecting the tissues from the metals-induced oxidative damage, though this effect was not enough to completely avoid Cd2+ and Cu2+ effect on certain antioxidant enzymes, though the precise mechanism of protection still needs to be elucidated.

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“…Thus it was shown that the variation of polyamines could not be dissociated from that of auxins in the inductive phase of rooting (Gaspar et al, 1996a(Gaspar et al, , 1997Faivre-Rampant et al, 2000a), where peroxidase activity obligatorily undergoes a reverse pattern. Polyamines do not intervene as such: a precise regulation of their anabolism and catabolism, with roles for the catabolic products, namely g-aminobutyrate (GABA) and H 2 O 2 , is needed (Hausman et al, 1997b;Martin-Tanguy et al, 1997;Aribaud et al, 1999). The same might be true for auxin (Blakesley, 1994).…”

Section: Plant Hormone Actionmentioning

confidence: 99%

Changing concepts in plant hormone action

Gaspar

Kevers

Faivre‐Rampant

et al. 2003

In Vitro Cell. Dev. Biol. - Plant

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A plant hormone is not, in the classic animal sense, a chemical synthesized in one organ, transported to a second organ to exert a chemical action to control a physiological event. Any phytohormone can be synthesized everywhere and can influence different growth and development processes at different places. The concept of physiological activity under hormonal control cannot be dissociated from changes in concentrations at the site of action, from spatial differences and changes in the tissue's sensitivity to the compound, from its transport and its metabolism, from balances and interactions with the other phytohormones, or in their metabolic relationships, and in their signaling pathways as well. Secondary messengers are also involved. Hormonal involvement in physiological processes can appear through several distinct manifestations (as environmental sensors, homeostatic regulators and spatio-temporal synchronizers, resource allocators, biotime adjusters, etc.), dependent on or integrated with the primary biochemical pathways. The time has also passed for the hypothesized ‘specific' developmental hormones, rhizocaline, canlocaline, and florigen: root, stem, and flower formation result from a sequential control of specific events at the right places through a coordinated control by electrical signals, the known phytohormones and nonspecific molecules of primary and secondary metabolism, and involve both cytoplasmic and apoplastic compartments. These contemporary views are examined in this review

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ODC-Mediated Biosynthesis and DAO-Mediated Catabolism of Putrescine Involved in the Induction of Rooting by Leaf Explants of Chrysanthemum morifolium Ramat In Vitro

Cited by 6 publications

References 0 publications

Involvement of polyamines in the adventitious rooting of micropropagated shoots of the apple rootstock MM106

Involvement of polyamines in the adventitious rooting of micropropagated shoots of the apple rootstock MM106

Polyamines and heavy metal stress: the antioxidant behavior of spermine in cadmium- and copper-treated wheat leaves

Changing concepts in plant hormone action

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