Ocular toxoplasmosis signs in mice embryo

Tedesco, Roberto; Vítor, Ricardo Wagner Almeida; Brandão, G.P.; Calabrese, Kátia da Silva

doi:10.1016/j.micron.2007.05.005

Cited by 7 publications

(6 citation statements)

References 27 publications

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“…The RPE presents an important role during T. gondii infection; those cells are responsible for phagocytic external segment cells during normal homeostasis, but their role during infections has been described [ 26 ]. It was also identified edema, inflammatory infiltrate of mononuclear cells, corroborating with literature and indicating an important role during T. gondii infection [ 1 , 27 ].…”

Section: Discussionsupporting

confidence: 85%

“…Infected embryos in E7 presented cell alterations which project nuclear layers in vitreal direction, characterizing chorioretinitis [ 1 ]. Same alterations were presented in other animal infection models [ 11 , 23 , 24 ].…”

Section: Discussionmentioning

confidence: 99%

“…After fixations, left eyes were dehydrated in a graded ethyl alcohol series and embedded for 12 h in historesin (glycol methacrylate, Technovit 7100) and ethyl alcohol (1:1); blocks were cut at a thickness of 1 µm in a semi-series (American Optical) and stained with 0.25% toluidine blue in 1% sodium borate. Slides were preserved in Erv-Mont (EasyPath ® ) covered by coverslips and observed in a Zeiss-Axioplan microscope [ 1–16 , 18 ].…”

Section: Methodsmentioning

confidence: 99%

“…The Toxoplasma gondii , the etiologic agent of toxoplasmosis [ 1 ], affects one third of the world population [ 2–9 ], and it is estimated that 20% have ocular infection [ 1 , 10 ]. Ocular toxoplasmosis has always been associated to congenital toxoplasmosis, where the mother contracts the parasite and transmits to the fetus; depending on the period that this infection occurs, the degree of complications varies, with more complicated cases during first months of the gestational period [ 2 , 4–6 ].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Toxoplasma gondii infection of chicken embryos causes retinal changes and modulates HSP90B1 gene expression: A promising ocular toxoplasmosis model

Násare

Tedesco

Cristovam

et al. 2015

EuJMI

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HSP90B1 is a gene that codifies heat shock protein 108 (HSP108) that belongs to a group of proteins induced under stress situation, and it has close relation with the nervous system, especially in the retina. Toxoplasma gondii causes ocular toxoplasmosis that has been associated with a late manifestation of the congenital toxoplasmosis although experimental models show that morphological alterations are already present during embryological development. Here, we used 18 eyes of Gallus domesticus embryos in 7th and 20th embryonic days to establish a model of congenital ocular toxoplasmosis, experimentally infected in its fifth day correlating with HSP90B1 gene expression. Embryos’ eyes were histologically evaluated, and gene expression was performed by real-time polymerase chain reaction (PCR). Our data showed parasite present in the choroid, unusual migration of retinal pigment epithelium, and chorioretinal scars, and a tendency to a lower expression of the HSP90B1 gene upon experimental infection. This is a promising model to better understand T. gondii etiopathogeny.

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Section: Discussionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Toxoplasma gondii infection of chicken embryos causes retinal changes and modulates HSP90B1 gene expression: A promising ocular toxoplasmosis model

Násare

Tedesco

Cristovam

et al. 2015

EuJMI

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“…A variety of pathological manifestations is observed in T. gondii infected murine models, ranging from meningoencephalitis to retinochoroiditis and inflammatory bowel disease (IBD) like [6] , [7] , [8] . Murine T. gondii oral infection is under polygenic control [9] , [10] .…”

Section: Introductionmentioning

confidence: 99%

Toxoplasma gondii Oral Infection Induces Intestinal Inflammation and Retinochoroiditis in Mice Genetically Selected for Immune Oral Tolerance Resistance

et al. 2014

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Toxoplasmosis is a worldwide disease with most of the infections originating through the oral route and generates various pathological manifestations, ranging from meningoencephalitis to retinochoroiditis and inflammatory bowel disease. Animal models for these pathologies are scarce and have limitations. We evaluated the outcome of Toxoplasma gondii oral infection with 50 or 100 cysts of the ME-49 strain in two lines of mice with extreme phenotypes of susceptibility (TS) or resistance (TR) to immune oral tolerance. Therefore, the aim of this study was to evaluate the behaviour of TS and TR mice, orally infected by T. gondii, and determine its value as a model for inflammatory diseases study. Mortality during the acute stage of the infection for TR was 50% for both dosages, while 10 and 40% of the TS died after infection with these respective dosages. In the chronic stage, the remaining TS succumbed while TR survived for 90 days. The TS displayed higher parasite load with lower intestinal inflammation and cellular proliferation, notwithstanding myocarditis, pneumonitis and meningoencephalitis. TR presented massive necrosis of villi and crypt, comparable to inflammatory bowel disease, with infiltration of lymphoid cells in the lamina propria of the intestines. Also, TR mice infected with 100 cysts presented intense cellular infiltrate within the photoreceptor layer of the eyes, changes in disposition and morphology of the retina cell layers and retinochoroiditis. During the infection, high levels of IL-6 were detected in the serum of TS mice and TR mice presented high amounts of IFN-γ and TNF-α. Both mice lineages developed different disease outcomes, but it is emphasized that TR and TS mice presented acute and chronic stages of the infection, demonstrating that the two lineages offer an attractive model for studying toxoplasmosis.

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Murine neonatal infection provides an efficient model for congenital ocular toxoplasmosis

Lahmar

Guinard

Sauer

et al. 2010

Experimental Parasitology

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Ocular toxoplasmosis signs in mice embryo

Cited by 7 publications

References 27 publications

Toxoplasma gondii infection of chicken embryos causes retinal changes and modulates HSP90B1 gene expression: A promising ocular toxoplasmosis model

Toxoplasma gondii infection of chicken embryos causes retinal changes and modulates HSP90B1 gene expression: A promising ocular toxoplasmosis model

Toxoplasma gondii Oral Infection Induces Intestinal Inflammation and Retinochoroiditis in Mice Genetically Selected for Immune Oral Tolerance Resistance

Murine neonatal infection provides an efficient model for congenital ocular toxoplasmosis

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