Ocular Surface Reconstruction With Autologous Nasal Mucosa in Cicatricial Ocular Surface Disease

Ocular Surface Reconstruction With a Tissue-Engineered Nasal Mucosal Epithelial Cell Sheet for the Treatment of Severe Ocular Surface Diseases

“…Most recently, Kim et al reported that autologous nasal mucosal tissue transplantation is a feasible treatment of patients with cicatricial OSD [17]. The ocular surface is composed of two different types of ECs (i.e., corneal and conjunctival), and goblet cells are diffusely located throughout the entire conjunctival area.…”

“…Several groups have previously reported that whole nasal mucosal tissue transplantation (both epithelial and subepithelial tissues) is, in part, effective for treating severe conjunctival mucin deficiency syndrome and eyelid and ocular surface reconstruction [16,17]. Because of the limited nasal donor site, we believe that the ex vivo expansion of goblet cells using a novel cell culture technique is a useful and promising procedure for developing the next generation of ocularsurface reconstruction surgeries.…”

Ocular Surface Reconstitution

“…Long-term follow-up, using impression cytology and hematoxylin and eosin and periodic acid-Schiff staining of biopsied tissue, demonstrated that functional goblet cells persisted in autologous nasal mucosa for up to 10 years after transplantation (Wenkel et al, 2000). Another study also reported similarly promising outcomes for patients with cicatricial ocular surface secondary to lye injury (Kim et al, 2010).…”

Concise Review: Limbal Epithelial Stem Cell Therapy: Controversies and Challenges

“…Of the limbus alternatives already mentioned (and in addition to oral mucosa), hair follicle, conjunctiva, dental pulp, and bone marrow are all feasible sources of autologous stem cells that could be harvested from patients with LSCD, some of these sources being more easily accessible with less invasive stem cell harvest than others. Nasal epithelium is another autologous source of stem cells which although has not yet been used in cultured stem cell therapy for the treatment of LSCD has shown promise when applied directly to the ocular surface of patients [50]. Advantages of using nasal oral mucosal epithelium are that it is easily accessible, expresses CK3, and contains goblet cells which secrete mucin important for tear film stabilization [50].…”

“…Nasal epithelium is another autologous source of stem cells which although has not yet been used in cultured stem cell therapy for the treatment of LSCD has shown promise when applied directly to the ocular surface of patients [50]. Advantages of using nasal oral mucosal epithelium are that it is easily accessible, expresses CK3, and contains goblet cells which secrete mucin important for tear film stabilization [50].…”