Ocular surface reconstruction using stem cell and tissue engineering

Nakamura, Takahiro; Inatomi, Tsutomu; Sotozono, Chie; Koizumi, Noriko; Kinoshita, Shigeru

doi:10.1016/j.preteyeres.2015.07.003

Cited by 65 publications

(63 citation statements)

References 135 publications

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“…A number of ocular surface diseases involve loss of cells on the surface and endothelium of the cornea (Ahmad, 2012). For all of these ocular diseases, researchers have proposed that stem cell-based therapy could be used to restore tissue health and function (Abu-Hassan et al, 2015; Al-Shamekh and Goldberg, 2014; Erbani et al, 2016; Mead et al, 2015; Nakamura et al, 2016; Roubeix et al, 2015; Zhu et al, 2016). One strategy for replacing lost cells is to transplant stem cells into the affected areas where they differentiate into the desired cell type and restore tissue/organ function (Blenkinsop et al, 2012).…”

Section: Stem Cells and Exosomesmentioning

confidence: 99%

Roles of exosomes in the normal and diseased eye

Klingeborn

Dismuke

Rickman

et al. 2017

Progress in Retinal and Eye Research

131

127

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Exosomes are nanometer-sized vesicles that are released by cells in a controlled fashion and mediate a plethora of extra- and intercellular activities. Some key functions of exosomes include cell-cell communication, immune modulation, extracellular matrix turnover, stem cell division/differentiation, neovascularization and cellular waste removal. While much is known about their role in cancer, exosome function in the many specialized tissues of the eye is just beginning to undergo rigorous study. Here we review current knowledge of exosome function in the visual system in the context of larger bodies of data from other fields, in both health and disease. Additionally, we discuss recent advances in the exosome field including use of exosomes as a therapeutic vehicle, exosomes as a source of biomarkers for disease, plus current standards for isolation and validation of exosome populations. Finally, we use this foundational information about exosomes in the eye as a platform to identify areas of opportunity for future research studies.

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Section: Stem Cells and Exosomesmentioning

confidence: 99%

Roles of exosomes in the normal and diseased eye

Klingeborn

Dismuke

Rickman

et al. 2017

Progress in Retinal and Eye Research

131

127

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“…Indeed, if one would need to engineer an autologous stroma, it would be difficult to isolate keratocytes from a stromal biopsy without generating a wound in the patient's cornea. On the other hand, limbal biopsies are safely performed by ophthalmologists for the harvest of epithelial stem cells for the treatment of ocular surface chemical burns (Nakamura et al, 2016;Pellegrini et al, 2011). The same type of limbal biopsy can be performed in order to isolate limbal stromal stem cells (Basu et al, 2014).…”

Section: Limbal Stem Cellsmentioning

confidence: 99%

Alternatives to eye bank native tissue for corneal stromal replacement

Brunette

Roberts

Vidal

et al. 2017

Progress in Retinal and Eye Research

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“…Despite many variables between studies (regarding inclusion/exclusion criteria, culture methods, transplantation techniques, and clinical outcome measures), long-term engraftment of autologous cultivated limbal epithelial cells has been shown to be good and the overall success rates of autologous CLET for unilateral LSCD with a follow-up period of at least 24 months were reported to amount to 72–76% 8, 15, 16 . In spite of these good clinical outcomes confirming CLET as an adequate therapy to successfully reconstruct the corneal surface in the majority of patients, long-term corneal regeneration in other series often proved less satisfactory due to recurrent mild neovascularisation of the corneal surface in many patients 17 . These complications might be caused by low quality of the graft or inadequate properties of transplanted progenitor cells 8, 11, 18 .…”

Section: Introductionmentioning

confidence: 99%

Laminin-511 and -521-based matrices for efficient ex vivo-expansion of human limbal epithelial progenitor cells

Polisetti

Sorokin²,

Okumura

et al. 2017

Sci Rep

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Optimization of culture conditions for human limbal epithelial stem/progenitor cells (LEPC) that incorporate the in vivo cell-matrix interactions are essential to enhance LEPC ex vivo-expansion and transplantation efficiency. Here, we investigate the efficacy of laminin (LN) isoforms preferentially expressed in the limbal niche as culture matrices for epithelial tissue engineering. Analyses of expression patterns of LN chains in the human limbal niche provided evidence for enrichment of LN-α2, -α3, -α5, -β1, -β2, -β3, -γ1, -γ2 and -γ3 chains in the limbal basement membrane, with LN-α5 representing a signature component specifically produced by epithelial progenitor cells. Recombinant human LN-521 and LN-511 significantly enhanced in vitro LEPC adhesion, migration and proliferation compared to other isoforms, and maintained phenotype stability. The bioactive LN-511-E8 fragment carrying only C-terminal domains showed similar efficacy as full-length LN-511. Functional blocking of α3β1 and α6β1 integrins suppressed adhesion of LEPC to LN-511/521-coated surfaces. Cultivation of LEPC on fibrin-based hydrogels incorporating LN-511-E8 resulted in firm integrin-mediated adhesion to the scaffold and well-stratified epithelial constructs, with maintenance of a progenitor cell phenotype in their (supra)basal layers. Thus, the incorporation of chemically defined LN-511-E8 into biosynthetic scaffolds represents a promising approach for xeno-free corneal epithelial tissue engineering for ocular surface reconstruction.

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Ocular surface reconstruction using stem cell and tissue engineering

Cited by 65 publications

References 135 publications

Roles of exosomes in the normal and diseased eye

Roles of exosomes in the normal and diseased eye

Alternatives to eye bank native tissue for corneal stromal replacement

Laminin-511 and -521-based matrices for efficient ex vivo-expansion of human limbal epithelial progenitor cells

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