Ocular surface epithelial and stem cell development

Wolosin, J. Mario; Budak, Murat T.; Akıncı, Mehmet-Ali

doi:10.1387/ijdb.041876jw

Cited by 110 publications

(63 citation statements)

References 63 publications

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“…Eye located in the basal epithelial layer along the basic membrane, and in the anterior stroma. 8,21 All normal human corneas examined displayed high concentrations of Cx43 with a characteristic immunolabeling pattern as described above. On the contrary, the immunostaining for Cx43 in six keratoconus corneas (corneas 2, 3, 4, 6, 7, and 8) was modified compared to normal corneas and this alteration was reflected in both the reduced signal intensity and the different immunolabelling pattern.…”

mentioning

confidence: 74%

“…8,9 Cx43 gap junctions mediate the intercellular diffusion of ions or molecules with a size of o1 kDa and contribute crucially to the regulation of corneal cell growth and differentiation, thereby having a significant impact on the maintenance of the corneal homoeostasis. 20,21 We found that normal corneas express a specific punctate immunostaining pattern for Cx43, Figure 2 Protein expression of Cx43 in human cornea. NC, normal cornea; KC, keratoconus cornea; RB, rat brain.…”

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confidence: 89%

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Reduced expression of the gap junction protein Connexin 43 in keratoconus

Gatzioufas

Charalambous

Thanos

2007

Eye

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Purpose The purpose of the present study was to examine whether keratoconus, which is a bilateral noninflammatory corneal ectasia with multifactorial aetiology, shows altered expression of Connexin (Cx43). Cx43 is an important gap junction protein that contributes crucially to epithelial and stromal integrity of cornea. Methods Eight keratoconic human corneal buttons were examined with immunohistochemistry and Western blotting and compared with eight normal human corneal buttons, to unravel changes in Cx43 expression. Results All normal corneas exhibited similar epithelial Cx43 expression patterns, with the protein located in the basal epithelial layer. In contrast, some keratoconic corneas showed an altered pattern of immunostaining and Western blotting confirmed a decreased expression of Cx43 in keratoconic corneas. Conclusions Our results indicate that a decrease in Cx43 amount together with functional alteration of the protein is associated with keratoconus pathophysiology However, these changes apply only to some of the corneas examined and may not generally account for the development of keratoconus.

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confidence: 74%

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confidence: 89%

Reduced expression of the gap junction protein Connexin 43 in keratoconus

Gatzioufas

Charalambous

Thanos

2007

Eye

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“…These new advances collectively make the corneal/limbal epithelia a prime tissue to practice regenerative medicine. For more detailed information on the aforementioned progresses regarding limbal epithelial SCs, the reader is encouraged to consult several reviews [35][36][37][38][39][40][41][42][43][44][45]. Further understanding of how self-renewal and fate decision of limbal epithelial SCs are regulated will undoubtedly unravel their additional therapeutic potentials in the future.…”

Section: Introductionmentioning

confidence: 99%

Niche regulation of corneal epithelial stem cells at the limbus

et al. 2007

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Among all adult somatic stem cells, those of the corneal epithelium are unique in their exclusive location in a defined limbal structure termed Palisades of Vogt. As a result, surgical engraftment of limbal epithelial stem cells with or without ex vivo expansion has long been practiced to restore sights in patients inflicted with limbal stem cell deficiency. Nevertheless, compared to other stem cell examples, relatively little is known about the limbal niche, which is believed to play a pivotal role in regulating self-renewal and fate decision of limbal epithelial stem cells. This review summarizes relevant literature and formulates several key questions to guide future research into better understanding of the pathogenesis of limbal stem cell deficiency and further improvement of the tissue engineering of the corneal epithelium by focusing on the limbal niche.

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“…It has been hypothesized that, for the purpose of self-renewal, stem cells reside in the conjunctiva and generate transient amplifying cells, and also differentiate into specialized cell types, including goblet cells and non-goblet cells, to maintain the survival and health of the conjunctiva (Wolosin et al, 2004). However, the localization of stem cells is still controversial.…”

Section: Discussionmentioning

confidence: 99%

潜在候选细胞构建组织工程的泪道上皮: 兔的组织学和细胞学研究

Xie

Cui

2015

J. Zhejiang Univ. Sci. B

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Abstract:Objective: Injury and deficiency of the lacrimal duct epithelium (LDE) can lead to a variety of lacrimal diseases. The purpose of this study was to characterize potential candidate cells for constructing a tissue-engineered LDE. Methods: Different areas of the conjunctiva and lacrimal duct tissue were removed from male adult New Zealand white rabbits for histological evaluation. Hematoxylin and eosin staining and immunohistochemical staining of cytokeratin AE1+AE3, cytokeratin 4, Ki-67, and MUC5AC were observed by light microscopy. The surface morphologies of different epithelial tissues and cellular structures were examined using field-emission scanning electron microscopy and transmission electron microscopy. Epithelial cells were isolated from tissues and identified by specific markers. In vitro, proliferative ability and Western blot analyses of the proliferating cell nuclear antigen (PCNA) of different epithelial cells cultured in identical environments were investigated and compared. Results: Histologically, the epithelial specific markers, cytokeratin AE1+AE3 and cytokeratin 4, were expressed in the conjunctiva epithelium and the LDE. Notably, highly proliferative cells stained with Ki-67 were concentrated under the epithelium in a dome structure of the posterior palpebral conjunctiva. Differentiated goblet cells were also found to a lesser extent in this region. Primary palpebral and fornical conjunctival epithelial cells (PFCECs), bulbar conjunctival epithelial cells (BCECs), and lacrimal duct epithelial cells (LDECs) were successfully separated from tissues. In vitro, rabbit PFCECs and LDECs grew faster and expressed more PCNA than BCECs. Conclusions: PFCECs are anatomically similar to LDECs. They also have similar morphological characteristics, immune phenotypes, and proliferation features. PFCECs are therefore potential candidate cells to replace LDECs in tissue engineering to treat lacrimal duct diseases.

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Ocular surface epithelial and stem cell development

Cited by 110 publications

References 63 publications

Reduced expression of the gap junction protein Connexin 43 in keratoconus

Reduced expression of the gap junction protein Connexin 43 in keratoconus

Niche regulation of corneal epithelial stem cells at the limbus

潜在候选细胞构建组织工程的泪道上皮: 兔的组织学和细胞学研究

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