Octopus, which owns the most advanced brain in invertebrates, has two members of vasopressin/oxytocin superfamily as in vertebrates

Takuwa‐Kuroda, Kyoko; Ukena, Kazuyoshi; Kanda, Atsuhiro; Minakata, Hiroyuki

doi:10.1016/s0167-0115(03)00151-4

Cited by 70 publications

(91 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The amino acid sequence of the purified material was X-Try-Phe-Arg-Asn-XPro-Ile-Gly (X is an unidentified residue). Sequencing profiles at the first and the sixth cycles in a protein sequencer were similar to those of CT: the first halfcysteine at position 1 did not appear on the chromatogram, whereas the second half-cysteine at position 6 was detected as two specific peaks: the dithiothreitol adduct of PTH-dehydroalanine and PTH-Cys (Nokihara et al 1990, Takuwa-Kuroda et al 2003 ) calculated for CT. Moreover, the fragmentation pattern of the isolated peptide in the TOF-MS/MS analysis was superimposable on that of CT.…”

Section: Isolation Of An Endogenous Ligand From Octopus Brainmentioning

confidence: 82%

“…We have previously shown that, in common with vertebrates, the common octopus, Octopus vulgaris, possesses two members of the OT/VP superfamily, OP and CT -the first time this was observed in invertebrates (Takuwa-Kuroda et al 2003). As OP and CT have different biological activities, the presence of specific receptors has been proposed.…”

Section: Discussionmentioning

confidence: 99%

“…Although the levels of expression in the ovary are very low, CTR may mediate some of the processes in fertilization and early embryonic development in Octopus. The oviduct expressed the CTR gene, but CT had no contractile effects on the oviduct (Takuwa-Kuroda et al 2003). The Octopus ovary sac contains various stages of oocytes.…”

Section: Expression Of Ctr Mrnamentioning

confidence: 99%

“…Although all vertebrate species except for cyclostomes have peptides from both the OT and the VP families, invertebrate species examined to date have usually possessed only a single peptide belonging to the OT/VP superfamily. We showed previously that, in addition to CT, Octopus possesses another member of the OT/VP superfamily, octopressin (OP) (Takuwa-Kuroda et al 2003). This was the first report of the co-occurrence of two members of the superfamily in a single invertebrate species.…”

Section: Introductionmentioning

confidence: 96%

“…This was the first report of the co-occurrence of two members of the superfamily in a single invertebrate species. Because OP, but not CT, induced contractile activity in smooth muscles of several Octopus tissues, we proposed that there are more than two distinct receptors in the octopus for members of the oxytocin/vasopressin superfamily (Takuwa-Kuroda et al 2003).…”

Section: Introductionmentioning

confidence: 98%

See 4 more Smart Citations

Cloning of Octopus cephalotocin receptor, a member of the oxytocin/vasopressin superfamily

Kanda

Takuwa‐Kuroda²,

Ukena³

et al. 2003

Journal of Endocrinology

View full text Add to dashboard Cite

We reported that the common octopus, Octopus vulgaris, in common with vertebrates, possesses two members of the oxytocin/vasopressin superfamily: octopressin (OP) and cephalotocin (CT). This was the first observation of its kind in invertebrates. As OP and CT have different biological activities, the presence of specific receptors has been proposed. We cloned the cDNA of an orphan receptor from Octopus brain and found it to encode a polypeptide of 397 amino acids that displays sequences characteristic of G-protein coupled receptors. The orphan receptor showed high homology to receptors of the oxytocin/vasopressin superfamily and seemed to conserve the agonist-binding pocket common to the oxytocin and vasopressin receptors. Xenopus oocytes that express the orphan receptor responded to the application of CT by an induction of membrane Cl currents coupled to the inositol phosphate/Ca 2+ pathway. OP and the other members of the oxytocin/vasopressin superfamily did not activate this receptor. HPLC fractionation of the Octopus brain extract combined with an oocyte assay yielded a single substance that was identical to CT. On the basis of these results, we conclude that the cloned receptor is the CT receptor (CTR). Expression of CTR mRNA in Octopus was detected in the central and the peripheral nervous systems, the pancreas, the oviduct and the ovary. This receptor may mediate physiological functions of CT in Octopus such as neurotransmission, reproduction and metabolism.

show abstract

Section: Isolation Of An Endogenous Ligand From Octopus Brainmentioning

confidence: 82%

Section: Discussionmentioning

confidence: 99%

Section: Expression Of Ctr Mrnamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 98%

See 3 more Smart Citations