OctoPartenopin: Identification and Preliminary Characterization of a Novel Antimicrobial Peptide from the Suckers of Octopus vulgaris

Abstract

Microorganism resistance to conventional antibiotics represents one of the major global health concerns. This paper focuses on a peptide (OctoPartenopin) extracted from suckers of Octopus vulgaris; bioassay-guided chromatographic fractionation was used to identify this sequence, which holds significant antibacterial activity against Gram-positive and Gram-negative bacteria. OctoPartenopin is encrypted within the calponin sequence and was associated with the high levels of proteolytic activity already reported in octopus arm suckers. We synthesized the parent peptide and four analogues; all peptide were tested for their antibacterial and antibiofilm activities. Preliminary antibiofilm experiments showed that that one of the analogues had the best activity in both inhibition and eradication of biofilm of all three microorganisms tested. The occurrence of OctoPartenopin in arm suckers provided novel speculative information on animal behavior, as concerns maternal care of fertilized eggs. Our results highlight that suckers are a rich source of multifaceted peptides to develop alternative antimicrobial agents and food preservatives.

Thais savignyi tissue extract: bioactivity, chemical composition, and molecular docking

“…For example, OctoPartenopin, a peptide extracted from the suckers of Octopus vulgaris , was used to synthesise four analogues that have proven functional in inhibiting the antibiofilm formation by S. aureus , P. aeruginosa , and C. albicans (Maselli et al. 2020 ). Moreover, the methanol/water tissue extracts of Nerita albicilla L. (Neritidae) and N. oryzarum Récluz (Neritidae) inhibited 93% and 95% of 40 different marine biofilm bacteria, respectively (Ramasamy and Murugan 2005 ).…”

Molluscan Compounds Provide Drug Leads for the Treatment and Prevention of Respiratory Disease

“…Further, the peptide extract reduced the expression of virulence factors (pyocyanin, pyoverdine, and protease) by >50% at 0.0195 mg/mL and acted synergistically with standard antibiotics ciprofloxacin and cefotaxime (up to 100% reduction in MICs) ( Table 5 ). As well, Maselli et al [ 170 ] recently reported antibiofilm activity (60% eradication at 80 μM) among peptides derived from the suckers of O vulgaris . Preliminary evidence indicates that Hcs from marine crustaceans can also inhibit biofilm formation (at 0.1 mg/mL) [ 180 , 181 , 182 ] and molluscan Hcs could function similarly.…”

“…Reasonable antibacterial activity against P. aeruginosa has been observed using some molluscan extracts and compounds ( Table 5 ). Examples include: tyriverdin (MIC 0.005 mg/mL) sourced from egg masses of D. orbita [ 149 ]; tartrolon E (MIC 0.31 mg/mL) a polyketide isolated from a molluscan gill symbiont [ 169 ]; peptides from O. vulgaris (MIC 50–300 μg/mL) [ 170 ]; Scutinin A isolated from Australian limpet Scutus antipodes (MIC 33 μM) [ 171 ]; 5’-deoxy-5’-methylthio-adenosine (MTA) from a dorid nudibranch [ 172 ]; and body extract of Drupella margariticola (MIC 0.07 mg/mL) [ 173 ] ( Table 5 , Figure 2 and Figure 4 ). Preliminary assays using mucus of H. aspera [ 174 ] and crude extract of Babylonia spirata [ 175 ], show antimicrobial activity although weaker than antibiotic controls ( Table 5 ).…”

Out of control: The need for standardised solvent approaches and data reporting in antibiofilm assays incorporating dimethyl-sulfoxide (DMSO)

“…Plates were incubated for 18–22 h at 37 °C with 5% CO 2 then read spectrophotometrically at OD 600 to measure antimicrobial activity. The same plates were evaluated for inhibition of biofilm formation, similar to [ [29] , [30] , [31] ]. Planktonic bacteria and media were carefully aspirated from wells before 200 μL PBS was added to each well then discarded and repeated to rinse.…”