1987
DOI: 10.1523/jneurosci.07-09-02811.1987
|View full text |Cite
|
Sign up to set email alerts
|

Octopamine- and cyclic AMP-stimulated phosphorylation of a protein in Limulus ventral and lateral eyes

Abstract: The biogenic amine octopamine (OCT) fulfills most of the criteria as a neurotransmitter of efferent fibers that project to lateral and ventral eyes of the horseshoe crab, Limulus polyphemus. OCT is synthesized by and released from the efferent fibers, and OCT mimics many of the effects of endogenous efferent activity. OCT stimulates an increase in intracellular adenosine 3′,5′-monophosphate (cAMP) in both ventral and lateral eyes, and many of the physiological effects of OCT in these eyes appear to be mediated… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1
1

Citation Types

6
68
0

Year Published

1998
1998
2013
2013

Publication Types

Select...
5
2

Relationship

0
7

Authors

Journals

citations
Cited by 37 publications
(74 citation statements)
references
References 30 publications
(32 reference statements)
6
68
0
Order By: Relevance
“…Lateral optic nerves were homogenized in 3-[ Nmorpholino]propanesulfonic acid (MOPS) homogenization buffer with protease inhibitors (Edwards and Battelle, 1987) and centrif uged for 30 min at 130,000 ϫ g in an airf uge (Beckman Instruments, Fullerton, CA). The supernatant was mixed with 0.25 volume of fresh 4ϫ SDS sample buffer (Laemmli, 1970) without bromophenol blue dye.…”
Section: Methodsmentioning
confidence: 99%
See 2 more Smart Citations
“…Lateral optic nerves were homogenized in 3-[ Nmorpholino]propanesulfonic acid (MOPS) homogenization buffer with protease inhibitors (Edwards and Battelle, 1987) and centrif uged for 30 min at 130,000 ϫ g in an airf uge (Beckman Instruments, Fullerton, CA). The supernatant was mixed with 0.25 volume of fresh 4ϫ SDS sample buffer (Laemmli, 1970) without bromophenol blue dye.…”
Section: Methodsmentioning
confidence: 99%
“…The supernatant was mixed with 0.25 volume of fresh 4ϫ SDS sample buffer (Laemmli, 1970) without bromophenol blue dye. The proteins were separated by SDS-PAGE on 7.5% gels (Edwards and Battelle, 1987) and blotted to nitrocellulose (MSI, Westboro, M A) (Towbin et al, 1979) in buffer containing 15% methanol and 0.005% ultrapure SDS. The blot was stained with Ponceau S (Aebershold et al, 1987), and the 122 kDa band was cut out.…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…The other three were incubated in the dark for 4h at room temperature in one of three conditions as detailed in the Materials and methods: (1) OCM, (2) OCM plus OA and IBMX, and (3) OCM plus forskolin and IBMX. In the presence of IBMX, OA and forskolin dramatically elevate cAMP in LEs (Edwards and Battelle, 1987;Dalal and Battelle, 2010). At the end of the incubation period, LE slices were fixed in the dark, and RhOps1-2, RhOps5, RhG q α and RhArr levels were quantified in each slice.…”
Section: Effects Of Elevating Camp On Changes On Rhabdomeral Protein mentioning
confidence: 99%
“…NINAC motor activity has not been demonstrated; therefore, it has been proposed that G q α translocation involves reversible associations of G q α with the cytoplasmic and rhabdomeral isoforms of NINAC (Montell, 2012). Limulus eyes express a single homolog of NINAC that is distributed throughout the photoreceptor, concentrated at the rhabdoms (Battelle et al, 2001), and is a major target for phosphorylation by the circadian clock via activation of cAMP-dependent protein kinase (Edwards and Battelle, 1987;Battelle et al, 1998;Kempler et al, 2007;Cardasis et al, 2007). Limulus myosin III is not a molecular motor ; however, based on the findings in Drosophila and the results presented here, it is interesting to speculate that the clock-and cAMP-dependent phosphorylation of Limulus myosin III plays a role in the clock-dependent translocation of G q α to the rhabdom.…”
Section: The Clock Regulates the Increase In Rhg Q α Levels In The Darkmentioning
confidence: 99%