Due to the large extent of the Amazon rainforest, research has historically focused on easily accessible locations. Thus, much of this region has little information available about its richness and plant distribution. Located south of the Madeira river, Municipality of Manicoré has a high number of phytophysiognomies, which may indicate the existence of a greater diversity of plant species. Therefore, from the compilation of previously collected records and based on botanical expeditions, this study evaluated the diversity and richness of angiosperms in Manicoré. We found 801 species, 409 genera and 106 families. Our data record 47 new occurrences for Amazonas State. Of these new occurrences, 12 are also the first record for the northern region. In addition, we have identified a new vine species of the genus Mandevilla Lindl. Given the well-known sample deficiency of the Amazon region, and considering the countless anthropogenic pressures that cities south of the Madeira river have been facing, knowledge of flora becomes increasingly urgent.