Ochrobactrum ciceri mediated induction of defence genes and antifungal metabolites enhance the biocontrol efficacy for the management of Botrytis leaf blight of Lilium under protected conditions

Priyanka, R.; Nakkeeran, S.

doi:10.1007/s42161-018-00212-3

Cited by 15 publications

(3 citation statements)

References 40 publications

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“…For strain Curtobacterium flaccumfaciens (XIEG29), the GC-MS resolved several antimicrobials compounds in the extracts as major peaks such as p-Xylene, Benzene, 1,3-dimethyl-, Benzaldehyde [ 102 ], Phenylethyl Alcohol [ 103 ], and also Phenol, 3,5-dimethoxy-, which is well-known as a polyphenolic compound with antifungal and antibacterial activities [ 104 ]; Diethyl trisulphide [ 105 ], 9-Octadecenamide [ 106 ], Pyrrolo [1,2-a] pyrazine-1,4-dione [ 107 ], 1-Docosene and 1-nonadecene are alkenes [ 108 ], and Nonacos-1-ene [ 109 ] ( Figure 5 B).…”

Section: Discussionmentioning

confidence: 99%

Synergistic Plant-Microbe Interactions between Endophytic Actinobacteria and Their Role in Plant Growth Promotion and Biological Control of Cotton under Salt Stress

Mohamad

Liu

et al. 2022

Microorganisms

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Bacterial endophytes are well-acknowledged inoculants to promote plant growth and enhance their resistance toward various pathogens and environmental stresses. In the present study, 71 endophytic strains associated with the medicinal plant Thymus roseus were screened for their plant growth promotion (PGP), and the applicability of potent strains as bioinoculant has been evaluated. Regarding PGP traits, the percentage of strains were positive for the siderophore production (84%), auxin synthesis (69%), diazotrophs (76%), phosphate solubilization (79%), and production of lytic enzymes (i.e., cellulase (64%), lipase (62%), protease (61%), chitinase (34%), and displayed antagonistic activity against Verticillium dahliae (74%) in vitro. The inoculation of strain XIEG05 and XIEG12 enhanced plant tolerance to salt stress significantly (p < 0.05) through the promotion of shoot, root development, and reduced the activities of antioxidant enzymes (SOD, POD, and CAT), compared with uninoculated controls in vivo. Furthermore, inoculation of strain XIEG57 was capable of reducing cotton disease incidence (DI) symptoms caused by V. dahliae at all tested salt concentrations. The GC-MS analysis showed that many compounds are known to have antimicrobial and antifungal activity. Our findings provide valuable information for applying strains XIEG05 and XIEG12 as bioinoculant fertilizers and biological control agent of cotton under saline soil conditions.

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Section: Discussionmentioning

confidence: 99%

Synergistic Plant-Microbe Interactions between Endophytic Actinobacteria and Their Role in Plant Growth Promotion and Biological Control of Cotton under Salt Stress

Mohamad

Liu

et al. 2022

Microorganisms

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“…Another example is Ochrobactrum ciceri MM17, which produces many different metabolites with antifungal activity, both diffusible and volatiles, such as propanoic acid, −hydroxymethyl ester, phthalic acid, hex-3-yl isobutyl ester and phthalic acid, hept-3-yl isobutyl ester (diffusible), and dimethyl trisulfide and pentadecane (VOC). These bacteria have also been tested against Botrytis, showing suppression of 77% of leaf blight [75]. Pantoea sp.…”

Section: Antimicrobial Moleculesmentioning

confidence: 99%

Mechanisms of Action of Microbial Biocontrol Agents against Botrytis cinerea

Roca-Couso

Flores-Félix

Rivas

2021

JoF

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Botrytis cinerea is a phytopathogenic fungus responsible for economic losses from USD 10 to 100 billion worldwide. It affects more than 1400 plant species, thus becoming one of the main threats to the agriculture systems. The application of fungicides has for years been an efficient way to control this disease. However, fungicides have negative environmental consequences that have changed popular opinion and clarified the need for more sustainable solutions. Biopesticides are products formulated based on microorganisms (bacteria or fungi) with antifungal activity through various mechanisms. This review gathers the most important mechanisms of antifungal activities and the microorganisms that possess them. Among the different modes of action, there are included the production of diffusible molecules, both antimicrobial molecules and siderophores; production of volatile organic compounds; production of hydrolytic enzymes; and other mechanisms, such as the competition and induction of systemic resistance, triggering an interaction at different levels and inhibition based on complex systems for the production of molecules and regulation of crop biology. Such a variety of mechanisms results in a powerful weapon against B. cinerea; some of them have been tested and are already used in the agricultural production with satisfactory results.

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“…(1) RT-qPCR (Oñate-Sánchez & Vicente-Carbajosa, 2008;Verdonk and Sullivan, 2013;Ambreetha, Chinnadurai, Marimuthu, & Balachandar, 2018;Arruabarrena et al, 2016;de Vries et al, 2020;Krithika et al, 2016;Lortzing et al, 2017;Mbaluto, Ahmad, Fu, Martínez-Medina, & van Dam, 2020;Priyanka et al, 2019;Sõmera et al, 2019;Touw et al, 2020;Wang, Severing, Koornneef, & Aarts, 2020), (2) RT-PCR (Kirkin et al, 2018;Nummert, Sõmera, Uffert, Abner, & Truve, 2017), (3) RACE-PCR (Zhou, Wilkens, Hanelt, & von Schwartzenberg, 2020), (4) direct PCR for detection of DNA virus (Arruabarrena et al, 2016), (5) DNA barcoding and preparation of RNA-based libraries (Sõmera et al, 2019), and (6) cDNA labeling and microarray hybridization (Lortzing et al, 2017).…”

Section: Understanding Resultsmentioning

confidence: 99%

Citrate‐Citric Acid RNA Isolation (CiAR) for Fast, Low‐Cost, and Reliable RNA Extraction from Multiple Plant Species and Tissues

Oñate-Sánchez

Verdonk

2021

Current Protocols

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RNA isolation is routinely carried out in many laboratories for different downstream applications. Although protocols for this can vary between labs depending on the specific plant species and tissues under study and the preferences of their researchers, these protocols usually include the use of volatile organic and toxic chemicals. As an alternative, several companies offer less hazardous RNA extraction kits, but these kits significantly increase the cost per sample and are thus not affordable for every lab, especially when a large number of samples is to be processed. We have previously described a fast and efficient method for RNA isolation from plant vegetative tissues that requires only two home-made, simple, inexpensive, and nontoxic buffers. Both buffers have low concentrations of citric acid and its sodium salt. The first buffer also contains a detergent to help with nucleic acid solubilization while keeping RNases inactive. The second buffer has sodium chloride at high molarity to separate protein from nucleic acids. RNA is precipitated, and contaminating DNA can then be optionally removed. Here, we describe and expand on this approach, which we call the citrate-citric acid RNA isolation, or CiAR, method. We provide a detailed description of the protocol, describe a modification to make it compatible with non-vegetative tissues, and compile and extend the number of species and tissues to which it can be applied.

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Ochrobactrum ciceri mediated induction of defence genes and antifungal metabolites enhance the biocontrol efficacy for the management of Botrytis leaf blight of Lilium under protected conditions

Cited by 15 publications

References 40 publications

Synergistic Plant-Microbe Interactions between Endophytic Actinobacteria and Their Role in Plant Growth Promotion and Biological Control of Cotton under Salt Stress

Synergistic Plant-Microbe Interactions between Endophytic Actinobacteria and Their Role in Plant Growth Promotion and Biological Control of Cotton under Salt Stress

Mechanisms of Action of Microbial Biocontrol Agents against Botrytis cinerea

Citrate‐Citric Acid RNA Isolation (CiAR) for Fast, Low‐Cost, and Reliable RNA Extraction from Multiple Plant Species and Tissues

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