Ochrobactrum anthropi bacteremia: Report of four cases and short review

Kern, Winfried V.; Oethinger, Margret; Kaufhold, Achim; Rozdzinski, Eva; Marre, R.

doi:10.1007/bf01712451

Cited by 74 publications

(60 citation statements)

References 16 publications

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“…As discussed, catheter line infection is the most common way by which O. anthropi causes infection in humans (13)(14)(15)(16)(17)(18). However, our patient had not received a central venous line or an arterial line during his hospitalization.…”

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“…O. anthropi is an aerobic, oxidase-positive, urease-positive, Gram-negative, motile, non-lactose-fermenting bacillus previously known as "Achromobacter group Vd" (12). Most cases of human disease due to this pathogen that have been reported have been associated with central venous catheter line infection (13)(14)(15)(16)(17)(18). However, this organism has also been reported as a cause of infective endocarditis (10,19,20), pancreatic abscess (21), puncture wound osteochondritis (22), endophthalmitis (23), urinary tract infection (24), meningitis (25), pelvic abscess (26), and osteomyelitis (27).…”

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Clinical Characteristics of Ochrobactrum anthropi Bacteremia

et al. 2013

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The clinical picture of Ochrobactrum anthropi infection is not well described because the infection is rare in humans and identification of the pathogen is difficult. We present a case of O. anthropi bacteremia that was initially misidentified as Ralstonia paucula and later identified by 16S rRNA sequencing and recA analysis. CASE REPORTA n 85-year-old man was admitted to our hospital to receive transcatheter arterial chemoembolization (TACE). He had liver cirrhosis (Child-Pugh score A) and hepatocellular carcinoma (HCC) caused by hepatitis C virus infection. His medical history included repeated radio frequency ablation and a total of nine TACE procedures for the treatment of HCC. He had also experienced urinary bladder and ureteral carcinoma.The patient was free from any complications; however, he suddenly developed high fever (39°C), chills, and rigors 12 days after TACE. Physical examination did not reveal any significant findings, but laboratory testing showed a highly inflammatory state: his white blood cell count was 17,700/mm 3 , and his C-reactive protein level was 4.5 mg/dl. Urine examination was normal, and whole-body computed tomography revealed no particular pathogenic lesion. An echocardiogram was not performed. Oral cefcapene pivoxil was prescribed for 1 week based on the suspicion of some forms of infection relating to TACE.Two sets of blood cultures were obtained at the time of high fever. Of these four bottles, two different aerobic bottles became positive for Gram-negative rods after an incubation period of 36 h. However, the patient's condition and laboratory measurements improved promptly, and he was discharged on day 16 without distinct diagnosis. Later, the organism was identified as Ralstonia paucula by the Microscan walkaway system (Siemens) with a concordance rate of 99.9%. Susceptibility testing showed the organism to be sensitive to imipenem, meropenem, amikacin, minocycline, and colistin but resistant to ␤-lactams such as piperacillin, piperacillin-tazobactam, ceftazidime, cefepime, aztreonam, levofloxacin, ciprofloxacin, gentamicin, and trimethoprim-sulfamethoxazole. Although the pathogen was resistant to the antibiotic prescribed, he responded well to the treatment and the antibiotic did not need to be changed.The organism had been rarely encountered at our hospital, and therefore the sample was sent to Kochi University for further identification. The two samples derived from different blood cultures were examined separately. Blood samples were inoculated onto agar media and incubated at 28°C. Pale yellow colonies grew in culture and released a popcorn-like smell. Almost the entire region of the 16S rRNA gene was amplified by colony PCR using a pair of primers (pr0R2, 5=-AGAGTTTGATCMTGGCTCAG-3=; 9Rev, 5=-AAGGAGGTGATCCAGCC-3=) and sequenced with the BigDye Terminator v3.1 cycle sequencing kit. The resulting sequences from the two samples were identical. BLAST analysis against the NCBI database revealed the organism not to be R. paucula but rather to be of the Ochrobactrum anthropi c...

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Clinical Characteristics of Ochrobactrum anthropi Bacteremia

et al. 2013

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“…The nonfermentative gram-negative bacteria Ochrobactrum anthropi is known as a potential human pathogen (Kern et al 1993). Strain AK was isolated from the gut of fourthinstar larvae just before pupation, the gut of females ready to emerge from pupae as well from midgut and hindgut of newly emerged adult females.…”

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confidence: 99%

Bacterial colonisation in the gut of Phlebotomus duboscqi (Diptera: Psychodidae): transtadial passage and the role of female diet

Volf

Kiewegová²,

Nemec³

2002

FOLIA PARASIT

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Abstract. Bacteria isolated from the gut of different developmental stages of Phlebotomus duboscqi Neveu-Lemaire, 1906 belonged almost all to aerobic or facultatively anaerobic gram-negative rods. In females, the highest bacterial counts were observed two days after bloodfeeding; seven days after bloodfeeding the bacterial counts returned to pre-feeding levels. Most isolates were identified phenotypically as Ochrobactrum sp. The distinctiveness and homogeneity of the phenotypic and genotypic characteristics of Ochrobactrum isolates indicated that they belonged to a single strain (designated AK). This strain was acquired by larvae from food and passaged transtadially: it was isolated from the guts of fourth-instar larvae shortly before pupation, from pupae as well from newly emerged females. Most other bacteria found in females were acquired from the sugar solution fed to adults. To determine if the midgut lectin activity may serve as antibacterial agent females were membrane-fed on blood with addition of inhibitory carbohydrates. No significant differences in bacterial infections were found between experimental and control groups and we suppose that the lectin activity has no effect on gram-negative bacteria present in sandfly gut.

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“…are potential human pathogens. Ochrobactrum anthropi bacteremia is usually associated with contaminated intravenous lines in immunocompromised human patients, water sources, and environmental conditions in hospitals (1,5,8,10,11). O. anthropi isolates have also recently been obtained from other sources such as water, concrete, soils, termites, feces, activated sludges, oil spills, etc.…”

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