Ochratoxin A reduces aflatoxin B1 induced DNA damage detected by the comet assay in Hep G2 cells

Corcuera, L.A.; Arbillaga, Leire; Vettorazzi, Ariane; Azqueta, Amaya; Ceráin, Adela López de

doi:10.1016/j.fct.2011.07.029

Cited by 58 publications

(39 citation statements)

References 37 publications

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“…So, the elevation of AFB 1 -cytotoxic effect caused by OTA tested at non toxic level (20nM) could be the consequence of such chemical characteristic interactive. Thus, ours results supported the findings reported on the capably of OTA to influence cell pathways involved in AFB 1 toxic action [16]. Indeed, OTA has complex mechanisms of action that include evocation of oxidative stress, bio-energetic compromise, mitochondrial impairment, inhibition of protein synthesis, production of DNA single strand breaks and formation of OTA-DNA adducts [18,[26][27][28].…”

Section: Discussionsupporting

confidence: 80%

“…These findings were partially supported by those more recently reported [16] which revealed that the co-exposure to OTA (tested at low or non genotoxic concentration) significantly decreased DNA damage induced by AFB 1 , not only in breaks and apurinic sites but also in FPG-sensitive sites suggesting antagonistic effect so the low level of OTA may influence AFB 1 toxic action [16]. However, in the same study [16], when AFB 1 was associated with OTA tested at high concentration or cytotoxic concentration that leaded to additive effect as previously reported [15]. Thus, the combined effect resulting from OTA and AFB 1 interaction is not always additive effect as reported previously [15] …”

Section: Introductionsupporting

confidence: 82%

“…Previously, it had been reported that the interaction between OTA and AFB 1 tested both at high levels leaded always to additive effect [15,16]. Surprisingly, when OTA was tested at very low or non toxic level, OTA decreased AFB 1 genotoxicity [16]. This finding poses an interesting question.…”

Section: Discussioncontrasting

confidence: 41%

“…Thus, there is a higher probability of being exposed to low doses of several mycotoxins at the same time throughout life than to a high level of only one mycotoxin. Logically, several studies were performed on varied combinations of mycotxins such as binary or tertiary combination on cells line in the aim to evaluate their simultaneous cytotoxic or genotoxic effect [5,7,[14][15][16]. Previously, it had been reported that the interaction between OTA and AFB 1 tested both at high levels leaded always to additive effect [15,16].…”

Section: Discussionmentioning

confidence: 99%

“…For example, studies focused on mycotoxins presence in foods from Côte d'Ivoire reported co-occurrence of aflatoxin B 1 , ochratoxin A, fumonisin and zearalenone in rice, maize, peanuts [21]. Since, multi-exposure could lead to additive, synergistic or antagonistic effects [5,7,15,16], several combinations of mycotxins such as binary or tertiary combination were tested on cells line in the aim to observe their simultaneous effect on cellular damages namely cytotoxicty, genotoxicity, or cells metabolism [5,7,15,16] but very few had concerned the mixture of AFB 1 and OTA [15,16].…”

Section: Introductionmentioning

confidence: 99%

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Low level of Ochratoxin A enhances Aflatoxin B1 Induced Cytotoxicity and Lipid Peroxydation in Both Human Intestinal (Caco-2) and Hepatoma (HepG2) Cells Lines

Halbin¹,

Brou²,

Dago³

2013

IJNFS

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Abstract:Aflatoxin B 1 (AFB 1 ) and ochratoxin A (OTA) are contaminants which co-occurred in the same food such as cereals. The few studies performed on their interactive effect had revealed additive or antagonistic cytotoxic effect according to cells endpoints and concentrations of both mycotoxins. The aim of the present study was to investigate in a possible influence of very low level of ochratoxin A in aflatoxin B 1 toxic action regarding cellular endpoints such as malonedialdehyde (MDA) production and cells viability as evaluated by lysosome and mitochondria integrities and cell lactate dehydrogenase (LDH) leakage. OTA (20nM) and AFB 1 were tested in combination in both human intestinal (Caco-2) and hepatoma (HepG2) cells lines. As results, OTA alone tested at 20nM was not cytotoxic and did not induce MDA production in both Caco-2 and HepG2 cells line. Interestingly, combined to AFB 1 (10µM), OTA enhanced markedly AFB 1 cytotoxic effect. OTA significantly increased cell lysosomes damage induced by AFB 1 from 24% to 38% (+14%) and from 28% to 43% (+15%) respectively in Caco-2 and HepG2 cells line (p<0.05). Similarly, OTA enhanced inhibition of mitochondria succinate dehydrogenase activity induced by AFB 1 until to +15% and +6% respectively in Caco-2 and HepG2 cells line (p<0.05). On cell necrosis marker, the mixture of OTA and AFB 1 induced more LDH leakage when compared to AFB 1 alone with increase of +14% and +12% respectively in Caco-2 and HepG2 cells line (p<0.05). Finally, on MDA production, AFB 1 + OTA induced more intensively MDA production when compared to AFB 1 alone with +49% and +110% of increasing in both Caco-2 and HepG2 cells line (p<0.01). Taken together, our results suggested that combined AFB 1 and OTA induced all the toxicities observed with the mycotoxins separately but more intensively suggesting synergistic or potentiating effect. Moreover, AFB 1 or its association with OTA had been found very potent in human hepatic cells HepG2 in necrosis induction but especially in lipids oxidative damage confirming oxidative stress as one of keys pathways in toxic action of AFB 1 .

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Section: Discussionsupporting

confidence: 80%

Section: Introductionsupporting

confidence: 82%

Section: Discussioncontrasting

confidence: 41%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Low level of Ochratoxin A enhances Aflatoxin B1 Induced Cytotoxicity and Lipid Peroxydation in Both Human Intestinal (Caco-2) and Hepatoma (HepG2) Cells Lines

Halbin¹,

Brou²,

Dago³

2013

IJNFS

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Toxic effects of a modified montmorillonite clay on the human intestinal cell line Caco-2

et al. 2013

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The incorporation of the natural mineral clay montmorillonite into polymeric systems enhances their barrier properties as well as their thermal and mechanical resistance, making them suitable for a wide range of industrial applications, e.g., in the food industry. Considering humans could easily be exposed to these clays due to migration into food, toxicological and health effects of clay exposure should be studied. In the present work, the cytotoxic effects induced by two different clays (the unmodified clay Cloisite(®) Na(+) , and the organically modified Cloisite(®) 30B) on Caco-2 cells were studied after 24 and 48 h of exposure. The basal cytotoxicity endpoints assessed were total protein content, neutral red uptake and a tetrazolium salt reduction. Our results showed that only Cloisite(®) 30B induced toxic effects. Therefore, the effects of subcytotoxic concentrations of this clay on the generation of intracellular reactive oxygen species, glutathione content and DNA damage (comet assay) were investigated. Results indicate that oxidative stress may be implicated in the toxicity induced by Closite(®) 30B, in regards of the increases in intracellular reactive oxygen species production and glutathione content at the highest concentration assayed, while no damage was observed in DNA. The most remarkable morphological alterations observed were dilated cisternae edge in the Golgi apparatus and nucleolar segregation, suggesting impairment in the secretory functions, which could be related to inhibition in the synthesis of proteins.

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Combinations of genotoxic tests for the evaluation of group 1 IARC carcinogens

Bhagat

2017

J of Applied Toxicology

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Ochratoxin A reduces aflatoxin B1 induced DNA damage detected by the comet assay in Hep G2 cells

Cited by 58 publications

References 37 publications

Low level of Ochratoxin A enhances Aflatoxin B1 Induced Cytotoxicity and Lipid Peroxydation in Both Human Intestinal (Caco-2) and Hepatoma (HepG2) Cells Lines

Low level of Ochratoxin A enhances Aflatoxin B1 Induced Cytotoxicity and Lipid Peroxydation in Both Human Intestinal (Caco-2) and Hepatoma (HepG2) Cells Lines

Toxic effects of a modified montmorillonite clay on the human intestinal cell line Caco-2

Combinations of genotoxic tests for the evaluation of group 1 IARC carcinogens

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