Ochratoxin A induces locomotor impairment and oxidative imbalance in adult zebrafish

Valadas, Jéssica; Sachett, Adrieli; Marcon, Matheus; Bastos, Leonardo M.; Piato, Ângelo

doi:10.1007/s11356-022-23692-4

Cited by 3 publications

(3 citation statements)

References 104 publications

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“…Despite findings from previous studies indicating alterations in social behavior due to exposure to different pesticides (Yan et al 2023; Liu et al 2023), our study did not observe any changes following exposure to CF in the SPT. Since social behavior is genetically preserved and has ontogenic nature (Buske and Gerlai 2011; Scerbina et al 2012), it is less vulnerable to low-impact modulations, such as the concentrations used in this study, where the highest concentration corresponds to 6% of the LC 50 of similar species, such as Carassius auratus, the goldfish (Dreosti et al 2015; Valadas et al 2023).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of behavioral and neurochemical changes induced by carbofuran in zebrafish (Danio rerio)

Oliveira,

Gallas-Lopes,

Chitolina

et al. 2024

Preprint

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Carbofuran (CF) is a carbamate class pesticide, widely used in agriculture for pest control in crops. On the other hand, CF is also detected as a contaminant in food and water sources. This pesticide has high toxicity in non-target organisms, and its presence in the environment poses a threat to the ecosystem. Research has revealed that this pesticide acts as an inhibitor of acetylcholinesterase (AChE), inducing an accumulation of acetylcholine in the brain. Consequently, this leads to the emergence of a cholinergic syndrome and various detrimental effects on human health. Nonetheless, our understanding of CF impact on the central nervous system remains elusive. Therefore, this study aimed to investigate the effects of CF on behavioral and neurochemical parameters in adult zebrafish. The animals were exposed for 96 hours to different concentrations of CF (5, 50, and 500 μg/L) and subjected to behavioral assessments in the novel tank test (NTT) and social preference test (SPT). Subsequently, they were euthanized, and their brains were used to evaluate neurochemical markers associated with oxidative stress and AChE levels. In the NTT and SPT, CF did not alter the evaluated behavioral parameters. Furthermore, CF did not affect the levels of AChE, non-protein sulfhydryl groups, and thiobarbituric acid reactive species in the zebrafish brain. However, further research is needed regarding the environmental exposure of this compound to non-target organisms.

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Section: Discussionmentioning

confidence: 99%

Evaluation of behavioral and neurochemical changes induced by carbofuran in zebrafish (Danio rerio)

Oliveira,

Gallas-Lopes,

Chitolina

et al. 2024

Preprint

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“…The sample size for biochemical assays was established based on the literature according to previous studies using zebrafish as an animal model (Marcon et al, 2019; Valadas et al, 2022).…”

Section: Methodsmentioning

confidence: 99%

“…After, the samples were gently homogenized on ice and centrifuged at 3,000 g for 10 min at 4 °C. The supernatants were transferred to microtubes and kept at - 80 °C until the analysis (Marcon et al, 2019; Valadas et al, 2022).…”

Section: Methodsmentioning

confidence: 99%

3-Nitropropionic acid induces histological and behavioral alterations in adult zebrafish: role of antioxidants on behavioral dysfunction

Wiprich,

Vasques,

Zaluski

et al. 2024

Preprint

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Huntingtons disease (HD) is a neurodegenerative disease marked by progressive motor and non-motor symptoms such as neuropsychiatric disruption and cognitive dysfunction. It has been reported that some pathogenic mechanisms resulting in neuronal cell death in this disease involve neurodegeneration and oxidative stress. 3-Nitropropionic acid (3-NPA), a natural toxin that promotes the irreversible suppression of mitochondrial complex II, has been used to understand the HD pathogenesis. This neurotoxin mimics the biochemical, central neurodegeneration, peripheral and behavioral phenotype alterations observed in HD. Here we investigated 3-NPA (60 mg/kg) effects on histological and oxidative stress parameters on brain and muscular tissues. We also evaluated the effects of three antioxidant compounds on 3-NPA-induced behavioral phenotypes in adult zebrafish. For the evaluation of the antioxidant effects, adult zebrafish were submitted to a single acute intraperitoneal injection of vitamin C, creatine, or melatonin following 3-NPA chronic administration (60 mg/kg). 3-NPA treatment caused neurodegeneration, but did not alter the muscular tissue. 3-NPA neither change thiobarbituric acid reactive substances (TBARS) nor nonprotein thiol levels. Vitamin C and creatine treatments recovered the hypolocomotion induced by 3-NPA. Also, vitamin C and melatonin treatments improved the memory dysfunction caused by 3-NPA. Altogether, our findings showed that the 3-NPA induces neurodegeneration in adult zebrafish, and the vitamin C, creatine, and melatonin are beneficial in managing HD-like behavioral phenotypes. Thus, these antioxidants could be thought as complementary pharmacotherapies for the treatment of late-stage HD symptoms.

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Neurotoxicity of ochratoxin A: Molecular mechanisms and neurotherapeutic strategies

Obafemi,

Adedara,

Rocha

2023

Toxicology

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Ochratoxin A induces locomotor impairment and oxidative imbalance in adult zebrafish

Cited by 3 publications

References 104 publications

Evaluation of behavioral and neurochemical changes induced by carbofuran in zebrafish (Danio rerio)

Evaluation of behavioral and neurochemical changes induced by carbofuran in zebrafish (Danio rerio)

3-Nitropropionic acid induces histological and behavioral alterations in adult zebrafish: role of antioxidants on behavioral dysfunction

Neurotoxicity of ochratoxin A: Molecular mechanisms and neurotherapeutic strategies

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