Ocena stężeń OPG, RANKL i markerów obrotu kostnego w surowicy oraz BMD u pacjentek leczonych z powodu osteoporozy pomenopauzalnej ranelinianem strontu i ibandronianem

Stuss, Michał; Sewerynek, Ewa; Król, Iwona; Stępień-Kłos, Wioletta; Jędrzejczyk, Sławomir

doi:10.5603/ep.a2016.0014

Cited by 11 publications

(1 citation statement)

References 49 publications

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“…For postmenopausal osteoporosis, oestrogen deficiency leads to upregulation of receptor activator of nuclear factor-kappa ligand (RANKL) and tumour necrosis factor-α, resulting in excessive bone resorption and rapid bone loss. 14 For senile osteoporosis, there is also a signiﬁcant reduction in bone formation due to osteoblastic dysfunction. Osteoblasts and adipocytes both differentiate from mesenchymal stem cells (MSCs), and there is a shift from osteoblastogenesis to adipogenesis in the bone marrow with increasing age, causing reduction in the life span of osteoblasts.…”

Section: Introductionmentioning

confidence: 99%

Identification of candidate genes in osteoporosis by integrated microarray analysis

Wang

Fei

et al. 2016

Bone & Joint Research

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ObjectivesIn order to screen the altered gene expression profile in peripheral blood mononuclear cells of patients with osteoporosis, we performed an integrated analysis of the online microarray studies of osteoporosis.MethodsWe searched the Gene Expression Omnibus (GEO) database for microarray studies of peripheral blood mononuclear cells in patients with osteoporosis. Subsequently, we integrated gene expression data sets from multiple microarray studies to obtain differentially expressed genes (DEGs) between patients with osteoporosis and normal controls. Gene function analysis was performed to uncover the functions of identified DEGs.ResultsA total of three microarray studies were selected for integrated analysis. In all, 1125 genes were found to be signiﬁcantly differentially expressed between osteoporosis patients and normal controls, with 373 upregulated and 752 downregulated genes. Positive regulation of the cellular amino metabolic process (gene ontology (GO): 0033240, false discovery rate (FDR) = 1.00E + 00) was significantly enriched under the GO category for biological processes, while for molecular functions, flavin adenine dinucleotide binding (GO: 0050660, FDR = 3.66E-01) and androgen receptor binding (GO: 0050681, FDR = 6.35E-01) were significantly enriched. DEGs were enriched in many osteoporosis-related signalling pathways, including those of mitogen-activated protein kinase (MAPK) and calcium. Protein-protein interaction (PPI) network analysis showed that the significant hub proteins contained ubiquitin specific peptidase 9, X-linked (Degree = 99), ubiquitin specific peptidase 19 (Degree = 57) and ubiquitin conjugating enzyme E2 B (Degree = 57).ConclusionAnalysis of gene function of identified differentially expressed genes may expand our understanding of fundamental mechanisms leading to osteoporosis. Moreover, significantly enriched pathways, such as MAPK and calcium, may involve in osteoporosis through osteoblastic differentiation and bone formation.Cite this article: J. J. Li, B. Q. Wang, Q. Fei, Y. Yang, D. Li. Identification of candidate genes in osteoporosis by integrated microarray analysis. Bone Joint Res 2016;5:594–601. DOI: 10.1302/2046-3758.512.BJR-2016-0073.R1.

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Section: Introductionmentioning

confidence: 99%