AbstractNicosulfuron is a herbicide used for plant protection. This paper presents the results of research on the physicochemical properties of three herbicidal preparations containing nicosulfuron as the active substance by measuring and comparing its concentration by the chromatographic method. These preparations are in the form of a concentrated suspension intended for dilution with water, and due to the fact that nicosulfuron contained in the tested preparations is sensitive to water quality, while checking the physicochemical properties of the preparations, a dispersion for soft (pH: 5–7) and hard (pH: 8–9) water was also determined. It is important to note that high content of mineral salts in water may have a negative effect on the biological activity of the substance. In addition, the use of herbicides does not only apply to the use of appropriate dilutions, which are effective and not harmful to the environment. However, it is also important to consider specific farmer and equipment used, so that the preparation is easy to use and does not adversely affect the kit used for spraying. This study has shown that depending on the preparation used based on the amount of active substance 40 g/l, the amount of nicosulfuron varies. The test indicators in the form of pH and density are similar, while during the tested dispersion, differences depending on the tested water were observed (sediments were observed in only one preparation tested). Differences depending on the occurrence of sediments in the water used, both at 0 and 24 h, are signaling in favor of the soft water. Therefore, it is important to assess the hardness of the water, which in turn guarantees a reduction of the amount of deposits and protection of agricultural equipment used for spraying.