Occurrence, virulence genes and antibiotic resistance of Escherichia coli O157 isolated from raw bovine, caprine and ovine milk in Greece

Σολωμάκος, Νικόλαος; Govaris, Α.; Angelidis, Apostolos S.; Pournaras, Spyros; Burriel, Angeliki R.; Kritas, S. K.; Papageorgiou, Dimitrios

doi:10.1016/j.fm.2009.06.002

Cited by 81 publications

(60 citation statements)

References 43 publications

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“…E. coli O157:H7 is of great significance to food borne diseases, because of its high virulence (it can cause disease at a dose of 5-50 cells) (Farrokh et al, 2013). Prevalence of STEC O157:H7 in bulk milk samples in our study (2 %, n=258) was in the range of studies, who worked on similar milk, carried in the USA, (0.02%, n=859) (Karns et al, 2007), (2.4% n=248) (Jayarao et al, 2006), Greece (0.7%, n = 950) (Solomakos et al, 2009) and Australian milk (varied from 1 -3%) (Dairy Australia, 2006). All presumptive O157:H7 E.…”

Section: Figsupporting

confidence: 51%

Characterization of Escherichia coli and other Enterobacteriaceae in producer-distributor bulk milk

Ntuli

Njage

Buys

2016

Journal of Dairy Science

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INTERPRETIVE SUMMARYCharacterization of E. coli and other Enterobacteriaceae in producer-distributor bulk milk, Ntuli, The direct sale of milk from producer-distributor (PD) to consumers is a common practice in many countries. We characterized E. coli and other Enterobacteriaceae in PD bulk milk sold at retail points in South Africa (SA). The study highlighted that there is a potential risk of transferring drug resistant pathogenic E. coli to consumers through the food chain.Information obtained from the study will be used to model the risk of pathogenic E. coli in PD milk and the proposal of most potentially effective mitigation efforts towards enhancing food security. ABSTRACTThe current study was undertaken to characterize Escherichia coli and other Enterobacteriaceae in raw and pasteurized producer-distributor bulk milk (PDBM). A total of 258 samples were collected from purchase points in 8 provinces in South Africa. The samples were tested for 1 antibiotic residues, phosphatase, total aerobic bacteria, coliforms and E. coli counts. Matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) was used for identification of isolates. E. coli isolates were characterized for virulence factors, antimicrobial resistance, serotypes and presumptive E. coli O157:H7. Antibiotic residues and alkaline phosphatase were detected in 2 % (n=258) and 21 % (n=104) of the samples respectively. A total of 729 isolates belonging to 21 genera and 59 species were identified. E. coli, Enterobacter cloacae, Klebsiella oxytoca and Raoultella ornithinolytica were the most abundant species. Spoilage Enterobacteriaceae species exceeded 50 % of the total isolates. E. coli was detected and isolated from 36 % of the milk samples. Thirty one E. coli isolates harbored virulence genes stx1/stx2 and 38 % (n=121) were presumptive O157:H7. Prevalence of samples with presumptive shigatoxin producing E. coli (STEC) was 10 %. Antimicrobial resistant E. coli isolates were detected in 70 % of the milk samples with 36 % of stx1/stx2 positive E. coli showing multi-drug resistance. Information obtained from the study will be used for modelling the public health risk posed by milkborne pathogens in PDBM, which in many cases is consumed by the poor and vulnerable population.

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Section: Figsupporting

confidence: 51%

Characterization of Escherichia coli and other Enterobacteriaceae in producer-distributor bulk milk

Ntuli

Njage

Buys

2016

Journal of Dairy Science

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“…Rahimi, Chaleshtori and Parsaei, (2010) found that 44.4% of the E. coli isolates from dairy products showed ampicillin resistance. Solomakos et al (2009) found that all of the E. coli isolates from milk were resistant to ampicillin, whereas Campos et al (2006) found increased resistance to ampicillin and cephalothin and resistance to tetracycline in E. coli isolated from milk and cheese.…”

Section: Discussionmentioning

confidence: 98%

“…Several studies of bacteria isolated from food have shown resistance to one or more antimicrobials, which is worrying because these microorganisms can be spread in the population as food contaminants (Lutgen et al, 2009;Solomakos et al, 2009;Dutil et al, 2010;Rahimi, Chaleshtori, Parsaei, 2010).…”

Section: Discussionmentioning

confidence: 99%

“…Heterogeneous profiles of antimicrobial resistance were also described by Rangel and Marin (2009) and Solomakos et al (2009) …”

Section: Discussionmentioning

confidence: 99%

“…coli has been isolated from various foodstuffs, such as meat, vegetables, milk, and milk derivatives, which can serve as a vehicle for the transmission of diseases by food (Lee et al, 2009;Solomakos et al, 2009;Brooks et al, 2012;Maffei et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

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Genetic heterogeneity of Escherichia coli isolated from pasteurized milk in State of Paraná, Brazil

Oltramari

Cardoso

Patussi

et al. 2014

Braz. J. Pharm. Sci.

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Food contamination caused by enteric pathogens is a major cause of diarrheal disease worldwide, resulting in high morbidity and mortality and significant economic losses. Bacteria are important agents of foodborne diseases, particularly diarrheagenic Escherichia coli. The present study assessed the genetic diversity and antimicrobial resistance of E. coli isolates from pasteurized milk processed in 21 dairies in northwestern State of Parana, Brazil. The 95 E. coli isolates were subjected to antimicrobial susceptibility testing according to the recommendations of the Clinical and Laboratory Standards Institute and assessed genotypically by Enterobacterial Repetitive Intergenic Consensus-Polymerase Chain Reaction (ERIC-PCR). The highest rate of resistance was observed for cephalothin (55.78%). ERIC-PCR revealed high genetic diversity, clustering the 95 bacterial isolates into 90 different genotypic patterns. These results showed a heterogeneous population of E. coli in milk samples produced in the northwestern region of Paraná and the need for good manufacturing practices throughout the processing of pasteurized milk to reduce the risk of foodborne illnesses. Uniterms

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Approaches to Assess the Risks/Modelling of Microbial Growth and Toxin Production

Murru¹,

Mercogliano²,

Cortesi³

et al. 2016

Microbial Toxins in Dairy Products

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Occurrence, virulence genes and antibiotic resistance of Escherichia coli O157 isolated from raw bovine, caprine and ovine milk in Greece

Cited by 81 publications

References 43 publications

Characterization of Escherichia coli and other Enterobacteriaceae in producer-distributor bulk milk

Characterization of Escherichia coli and other Enterobacteriaceae in producer-distributor bulk milk

Genetic heterogeneity of Escherichia coli isolated from pasteurized milk in State of Paraná, Brazil

Approaches to Assess the Risks/Modelling of Microbial Growth and Toxin Production

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