Occurrence of veterinary drug residues in farmed fishery products in South Korea

Kang, Hui-Seung; Lee, Soo Bin; Shin, Dasom; Jeong, Jin‐Yong; Hong, Jin-Hwan; Rhee, Gyu-Seek

doi:10.1016/j.foodcont.2017.09.019

Cited by 75 publications

(28 citation statements)

References 22 publications

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“…However, antimicrobial residues in the fish farming area are also responsible for the bacterial resistance (Kang et al . ).…”

Section: Resultsmentioning

confidence: 97%

“…The resistance phenomenon might be due to the repetitive use of unauthorized or banned antimicrobials by the fish producers in Korea or, the cross resistance between the antimicrobials due to the common resistance mechanisms and co-selection of resistance determinants located in the same genetic elements (Lukkana et al 2012;Kim et al 2014). However, antimicrobial residues in the fish farming area are also responsible for the bacterial resistance (Kang et al 2018).…”

Section: Resultsmentioning

confidence: 99%

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Multidrug resistant Aeromonas spp. isolated from zebrafish (Danio rerio): antibiogram, antimicrobial resistance genes and class 1 integron gene cassettes

Hossain

Dahanayake

Silva

et al. 2019

Letters in Applied Microbiology

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Aeromonas spp. are Gram‐negative opportunistic bacteria which have been commonly associated with fish diseases. In this study, antibiogram, antimicrobial resistance genes and integrons of 43 zebrafish‐borne Aeromonas spp. were studied. The isolates were identified as six Aeromonas species (A. veronii biovar veronii (n = 26), A. veronii biovar sobria (n = 3), A. hydrophila (n = 8), A. caviae (n = 3), A. enteropelogenes (n = 2) and A. dhakensis (n = 1)). Antibiogram of the isolates indicated that most of them were resistant to amoxicillin (100·00%), nalidixic acid (100·00%), oxytetracycline (100·00%), ampicillin (93·02%), tetracycline (74·42%), rifampicin (67·44%) and imipenem (65·15%). Multiple antimicrobial resistance (MAR) index values ranged from 0·19–0·44 to 90·70% isolates showed multidrug resistance. PCR of antimicrobial resistance genes revealed that the tetracycline resistance gene (tetA) was the most predominant (67·44%) among the isolates. The qnrS (53·49%), tetB (30·23%), tetE (30·23%), qnrB (23·26%) and aac(6’)‐Ib‐cr (4·65%) genes were also detected. Class 1 integrase (IntI1) gene was found in 46·51% of the isolates. Two types of class 1 integron gene cassette profiles (qacG‐aadA6‐qacG and drfA1) were identified. The results showed that zebrafish‐borne aeromonads can harbour different types of antimicrobial resistance genes and class 1 integrons. Significance and Impact of the Study Aeromonas spp. are important pathogens found in diverse environments. Antimicrobial resistance genes and integrons of ornamental fish‐borne Aeromonas spp. are not well studied. The antibiogram, antimicrobial resistance genes and class 1 integrons of Aeromonas spp. isolated from zebrafish were characterized for the first time in Korea. The prevalence of tetracycline resistance genes, plasmid‐mediated quinolone resistance genes and class 1 integron gene cassettes were observed among the isolates. The qacG‐aadA6‐qacG gene cassette was identified for the first time in Aeromonas spp. The results suggest that the wise use of antimicrobials is necessary for the better management of the ornamental fish.

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“…However, antimicrobial residues in the fish farming area are also responsible for the bacterial resistance (Kang et al . ).…”

Section: Resultsmentioning

confidence: 97%

Section: Resultsmentioning

confidence: 99%

Multidrug resistant Aeromonas spp. isolated from zebrafish (Danio rerio): antibiogram, antimicrobial resistance genes and class 1 integron gene cassettes

Hossain

Dahanayake

Silva

et al. 2019

Letters in Applied Microbiology

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“…3) Residue occurrence (R r, f ): It was calculated based on the research data conducted by the National Institute of Food and Drug Safety Evaluation in 2014-2016 (Kang et al 2018;Shin et al 2018). In addition, the number of noncompliant sample and detection rate were utilized for the residue occurrence.…”

Section: Compilation Of Data For Risk Analysismentioning

confidence: 99%

“…Thus, the authorized veterinary drugs such as antibiotics and anthelmintics have been continuously used to prevent diseases in fishery farm (Kim et al 2019). However, overuse or noncompliance of withdrawal period of veterinary drugs has been increasing due to the shift in farming environment such as changes in climate and incidence of antibiotic resistant bacteria (Kang et al 2018).…”

Section: Introductionmentioning

confidence: 99%

Risk-based approach to develop a national residue program: prioritizing the residue control of veterinary drugs in fishery products

et al. 2019

Self Cite

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Veterinary drugs are widely used to protect production-related diseases and promote the growth of farmed fish. The use of large amounts of veterinary drugs may have potential risk and cause adverse effects on both humans and the environment. In this study, we developed risk-based ranking based on a scoring system to be applied in the national residue program. In this approach, the following three factors of veterinary drugs that may occur as residues in fishery products were considered: potency (acceptable daily intake), usage (number of dose and withdrawal period), and residue occurrence. The overall ranking score was calculated using the following equation: potency × usage (sum of the number of sales and withdrawal period) × residue occurrence. The veterinary drugs that were assigned high score by applying this approach were enrofloxacin, amoxicillin, oxolinic acid, erythromycin, and trimethoprim. The risk-based approach for monitoring veterinary drugs can provide a reliable inspection priority in fishery products. The developed ranking system can be applied in web-based systems and residuemonitoring programs and to ensure safe management of fishery products in Korea.

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“…According to previous studies, liquid chromatography-tandem mass spectrometry (LC-MS/MS) is a reliable method for the simultaneous multi-residue detection of TEB and IND in oranges, carrots and spinach (Sack et al, 2011); wine grapes (Rose, Lane, & Jordan, 2009); fruit juices (Tran et al, 2012); hot peppers (Zhu, Zhao, Nan Feng, & Han Kim, 2014); and other matrices (Alder, Greulich, Kempe, & Vieth, 2006;Kang et al, 2018). Although previous studies performed simultaneous analysis of residual levels of TEB and IND in agricultural products, only two methods have been reported for the analysis of terrestrial animal products such as meat and milk using gel-permeation chromatography (GPC) clean-up (Pang et al, 2009) and dispersive solid-phase extraction (d-SPE) extraction (Anagnostopoulos, Bourmpopoulou, & Miliadis, 2013).…”

mentioning

confidence: 99%

Residue analysis of tebufenozide and indoxacarb in chicken muscle, milk, egg and aquatic animal products using liquid chromatography–tandem mass spectrometry

Choi

Zheng

El‐Aty

et al. 2019

Biomedical Chromatography

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We developed an analytical method using liquid–liquid extraction (LLE) and liquid chromatography–tandem mass spectrometry (LC‐MS/MS) to detect and quantify tebufenozide (TEB) and indoxacarb (IND) residues in animal and aquatic products (chicken muscle, milk, egg, eel, flatfish, and shrimp). The target compounds were extracted using 1% acetic acid (0.1% acetic acid for egg only) in acetonitrile and purified using n‐hexane. The analytes were separated on a Gemini‐NX C18 column using (a) distilled water with 0.1% formic acid and 5 mm ammonium acetate and (b) methanol with 0.1% formic acid as the mobile phase. All six‐point matrix‐matched calibration curves showed good linearity with coefficients of determination (R2) ≥0.9864 over a concentration range of 5–50 μg/kg. Intra‐ and inter‐day accuracy was expressed as the recovery rate at three spiking levels and ranged between 73.22 and 114.93% in all matrices, with a relative standard deviation (RSD, corresponding to precision) ≤13.87%. The limits of quantification (LOQ) of all target analytes ranged from 2 to 20 μg/kg, which were substantially lower than the maximum residue limits (MRLs) specified by the regulatory agencies of different countries. All samples were collected from different markets in Seoul, Republic of Korea, and tested negative for tebufenozide and indoxacarb residues. These results show that the method developed is robust and may be a promising tool to detect trace levels of the target analytes in animal products.

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Occurrence of veterinary drug residues in farmed fishery products in South Korea

Cited by 75 publications

References 22 publications

Multidrug resistant Aeromonas spp. isolated from zebrafish (Danio rerio): antibiogram, antimicrobial resistance genes and class 1 integron gene cassettes

Multidrug resistant Aeromonas spp. isolated from zebrafish (Danio rerio): antibiogram, antimicrobial resistance genes and class 1 integron gene cassettes

Risk-based approach to develop a national residue program: prioritizing the residue control of veterinary drugs in fishery products

Residue analysis of tebufenozide and indoxacarb in chicken muscle, milk, egg and aquatic animal products using liquid chromatography–tandem mass spectrometry

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