Occurrence of Trophoblasts in the Blood of Toxaemic Patients

Jäämeri, K. E. U.; Koivuniemi, A; Carpen, E

doi:10.1159/000303403

Cited by 19 publications

(19 citation statements)

References 2 publications

(2 reference statements)

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“…The mean length of SNAs was 72 ± 21 μm (range 42-116), which is comparable to the size of SNAs reported in various vascular beds in vivo (decidual vein 50 μm [16] , broad ligament vein 40-75 μm [17] , uterine vein 10-200 μm [20,24,35] , lungs 12-150 μm [22,[36][37][38] and peripheral circula- [16,17,[24][25][26][27][28] ). In addition, we observed 3 general morphologies/shapes for our SNAs; teardrop, rounded and irregular, which are also the same as those reported for SNAs in vivo [24] .…”

Section: Dna Content and Metabolic Activity Of Snassupporting

confidence: 70%

“…SNAs that contain multiple copies of the fetal genome can be isolated from maternal blood [16,17,20,[24][25][26][27][28]35] , albeit currently with poor efficiency [16,18,25] , and may be a source of DNA for prenatal screening. [29] .…”

Section: Discussionmentioning

confidence: 99%

“…It is estimated that approximately 100,000 SNAs are shed into the maternal blood each day in normal pregnancy [19,20] . Thus, there are large numbers of SNAs entering the maternal blood daily, each of which contains many copies of the fetal genome, making SNAs a potentially attractive target for genetic analysis.…”

mentioning

confidence: 99%

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Detection of Fetal Sex, Aneuploidy and a Microdeletion from Single Placental Syncytial Nuclear Aggregates

Holland

Kroneis²,

El‐Heliebi³

et al. 2016

Fetal Diagn Ther

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Objectives: A key problem in prenatal screening using extra-embryonic cells is the feasibility of extracting usable DNA from a small number of cells. Syncytial nuclear aggregates (SNAs) are multinucleated structures shed from the placenta. This study assesses the potential of SNAs as a source of fetal DNA for the detection of genetic abnormalities. Methods: SNAs were collected in vitro. Whole-genome amplification was used to amplify DNA from single SNAs, and DNA quality and quantity was assessed by spectrophotometry and PCR. Confocal microscopy was used to count nuclei within SNAs, determine metabolic activity and investigate DNA damage. Fetal sex and chromosomal/genetic abnormalities were investigated with array-comparative genomic hybridization (aCGH). Results: DNA was amplified from 81% of the individual SNAs. A mean of 61 ± 43 nuclei were found per SNA. DNA strand breaks were found in 76% of the SNAs. Seventy-five percent of SNAs yielded whole-genome-amplified DNA of sufficient quality for aCGH after storage and shipping. Individual SNAs from the same pregnancy reliably gave the same chromosomal profile, and fetal sex and trisomies could be detected. A microdeletion was detected in one pregnancy. Conclusion: SNAs could provide a source of extra-embryonic DNA for the prenatal screening/diagnosis of fetal sex and chromosomal and sub-chromosomal genetic abnormalities.

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Section: Dna Content and Metabolic Activity Of Snassupporting

confidence: 70%

Section: Discussionmentioning

confidence: 99%

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Detection of Fetal Sex, Aneuploidy and a Microdeletion from Single Placental Syncytial Nuclear Aggregates

Holland

Kroneis²,

El‐Heliebi³

et al. 2016

Fetal Diagn Ther

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“…Such a finding was already mentioned by Kaltenbach et al [14], It seems logical to postulate that the increase of 3H-thymidine incorpora tion in cytotrophoblast reflects a reparation or replacement process. It is well known that syncytiotrophoblast is easily damaged in preeclampsia [4,13] or in hypoxic states [5] and results in abundant syncytial sprout for mation followed by knot detachment into maternal circulation [11].…”

Section: Discussionmentioning

confidence: 99%

Cellular Proliferation in Villi of Normal and Pathological Pregnancies

Hustin¹,

Foidart

Lambotte³

1984

Gynecol Obstet Invest

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DNA and protein synthesis have been studied in placental villi from normal and pathological cases by in vitro incorporation of tritiated thymidine or tritiated proline and subsequent counting or autoradiography. It appeared that cytotrophoblastic DNA synthesis continued until term and that it was particularly important in preeclampsia cases and in cases of villous immaturity (rhesus sensitization). Protein synthesis was also increased in preeclampsia and seemed to be due to a very active cytotrophoblastic metabolism. The most interesting finding was that in preeclampsia cases, especially when intrauterine growth retardation was superimposed, villous capillary endothelial cell proliferation was as prominent as in cases where villous maturation was not achieved. Such results are highly suggestive of an important compensatory proliferative mechanism in the placentae of preeclamptic women.

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“…Noninvasive methods of prenatal diagno sis using detection of fetal cells in maternal blood represent a new Field of applications under development [1], Trophoblast cells in maternal blood originate from anucleatcd, mononucleated and multinucleated trophoblasts desquamating in the intervillous or retroplacental maternal blood [2], Syncytiotrophoblastic and cytotrophoblastic cells have been observed in the venous uterine maternal blood [3,4], inferior cava blood and lung arteries from toxemic patients [5], Enrich ment and identification of fetal cells are possi ble by using monoclonal antibodies (Mabs) and cell sorter [2,6,7] or immunomagnetic beads [8], Analysis of fetal cells isolated from maternal blood can be realized by polymerase chain reaction or by fluorescence in situ hy-bridization (FISH) [9,10]. In this report, we have used the combined method of immunomagnetic beads and FCM to sort fetal cells from peripheral maternal blood, and FISH analysis to detect 47.XYY aneuploidy which had been previously diagnosed after amnio centesis.…”

Section: Introductionmentioning

confidence: 99%

Detection of 47, XYY Trophoblast Fetal Cells in Maternal Blood by Fluorescence in situ Hybridization after Using Immunomagnetic Lymphocyte Depletion and Flow Cytometry Sorting

Cacheux

Milési-Fluet²,

Tachdjian

et al. 1992

Fetal Diagn Ther

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Fluorescence in situ hybridization (FISH) allows to diagnose aneuploidy in uncultured interphase nuclei. This rapid method of chromosomal analysis associated with cell-sorting techniques was realized on 47, XYY fetal cells isolated from maternal blood. Trophoblast cells were sorted by combining immunomagnetic removal of maternal lymphocytes and flow cytometry sorting using antitrophoblast monoclonal antibodies. Cells were sorted directly on slides and analyzed by FISH with a Y-centromeric probe. Among 1,387 examinable nuclei, 59 (4.25%) showed one single or two Y-specific domains.

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Occurrence of Trophoblasts in the Blood of Toxaemic Patients

Cited by 19 publications

References 2 publications

Detection of Fetal Sex, Aneuploidy and a Microdeletion from Single Placental Syncytial Nuclear Aggregates

Detection of Fetal Sex, Aneuploidy and a Microdeletion from Single Placental Syncytial Nuclear Aggregates

Cellular Proliferation in Villi of Normal and Pathological Pregnancies

Detection of 47, XYY Trophoblast Fetal Cells in Maternal Blood by Fluorescence in situ Hybridization after Using Immunomagnetic Lymphocyte Depletion and Flow Cytometry Sorting

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