Occurrence of transferable antibiotic resistances in commercialized ready-to-eat mealworms ( Tenebrio molitor L.)

Osimani, Andrea; Cardinali, Federica; Aquilanti, Lucia; Garofalo, Cristiana; Roncolini, Andrea; Milanović, Vesna; Pasquini, Marina; Tavoletti, Stefano; Clementi, Francesca

doi:10.1016/j.ijfoodmicro.2017.10.009

Cited by 32 publications

(51 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Regarding Enterobacteriaceae, the sporadic occurrence of viable counts above 1 log cfu/g was in accordance with values previously detected in ready‐to‐eat mealworms and grasshoppers commercialized in the EU (Osimani et al., , ).…”

Section: Resultssupporting

confidence: 90%

“…The average load of this microbial group was similar to that reported by Osimani et al. (, ) for the same microorganisms in ready‐to‐eat mealworms and grasshoppers marketed in the EU.…”

Section: Resultssupporting

confidence: 88%

“…Regarding spore‐forming bacteria, many authors have previously reported the stable occurrence of these microorganisms in edible insects (Garofalo et al., ; Klunder, Wolkers‐Rooijackers, Korpela, & Nout, ; Osimani et al., , , ; Stoops et al., ; Vandeweyer et al., , ; Wynants et al., ). Indeed, spore‐forming bacteria are able to produce endospores that are characterized by heat resistance, allowing them to survive processing treatments.…”

Section: Resultsmentioning

confidence: 99%

“…Regarding edible insects, to date only a few studies have investigated the occurrence of transferable AR genes in this novel food source (Milanović et al., , ; Osimani et al., , , ; Vandeweyer et al., ). These studies revealed the massive presence of genes conferring resistance to tetracyclines and other antibiotics.…”

Section: Introductionmentioning

confidence: 99%

“…The genes under study were selected among those having the highest risk levels, based on the likelihood of their introduction into human pathogens, and the relative consequences of such an event on human health (Martinez, Coque, & Baquero, ). In fact, these genes are widespread among both commensal bacteria (Aarts & Margolles, ) and human pathogens typically found in food‐producing animals, aquaculture, fruits, vegetables (Rolain, ), and even edible insects (Milanović et al., ; Osimani et al., , , ; Vandeweyer et al., ). In the present study, the bacterial DNA directly extracted from insects was subjected to optimized polymerase chain reaction (PCR) and nested‐PCR assays for the qualitative detection of the selected AR genes.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Investigating Antibiotic Resistance Genes in Marketed Ready‐to‐Eat Small Crickets (Acheta domesticus)

Roncolini

Cardinali

Aquilanti

et al. 2019

Journal of Food Science

Self Cite

View full text Add to dashboard Cite

The present investigation was aimed at evaluating the occurrence of transferable genes conferring resistance to tetracyclines, macrolide‐lincosamide‐streptogramin B (MLSB), vancomycin, beta‐lactams, and aminoglycosides in 32 samples from eight batches of ready‐to‐eat crickets (Acheta domesticus) commercialized by four European Union producers (two batches per producer). Bacterial DNA extracted directly from the insects was subjected to optimized polymerase chain reaction (PCR) and nested‐PCR assays for the qualitative detection of 12 selected antibiotic resistance (AR) genes. Microbial enumeration demonstrated high counts of spore‐forming bacteria and total mesophilic aerobes. Statistical analyses revealed significant differences between different producers and insect batches. Regarding AR genes, a high prevalence of genes conferring resistance to tetracycline [tet(M), tet(O), tet(K), tet(S)] was observed, together with the presence of genes conferring resistance to erythromycin [erm(B), erm(C)], beta‐lactams (blaZ and mecA), and aminoglycosides [aac(6’)‐Ie aph(2”)‐Ia]. We performed a principal component analysis based on the AR gene frequencies that differentiated samples of batch 1 from those of batch 2. This analysis provided evidence for a difference between the producer from France and all the other producers among the batch 1 samples. Practical application Overall, an intrabatch variation was seen in the transferable resistances among different producers. This evidence, coupled with the observed differences in the viable counts, suggests a low standardization of the production processes. Hence, a prudent use of antimicrobials during the rearing of insects destined for human consumption is strongly recommended, as well as a need for a full standardization of production technologies.

show abstract

Section: Resultssupporting

confidence: 90%

“…The average load of this microbial group was similar to that reported by Osimani et al. (, ) for the same microorganisms in ready‐to‐eat mealworms and grasshoppers marketed in the EU.…”

Section: Resultssupporting

confidence: 88%