Occurrence of thermotolerant<i>Campylobacter</i>spp. at different stages of the poultry meat supply chain in Argentina

Mv, Zbrun; Romero-Scharpen, Analía; Olivero, Carolina Raquel; Rossler, Eugenia; Lp, Soto; Rosmini, M.R.; Gj, Sequeira; Signorini, Marcelo; Ls, Frizzo

doi:10.1080/00480169.2013.817294

Cited by 19 publications

(8 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…From Brazil and Costa Rica, 100.0% respectively 80.0% of the flocks were reported to be positive for Campylobacter when ceca samples were studied (Giombelli and Gloria, 2014 ; Zumbaco-Gutiérrez et al, 2014 ). On the other hand, in Argentina and Chile Campylobacter was found in 33.3% of samples (Rivera et al, 2011 ; Zbrun et al, 2013a ) while in Peru Tresierra-Ayala et al ( 1995 ) reported a prevalence of 35%. Other tropical countries such as Vietnam and South Africa have reported a prevalence of 31.9 and 14.2% respectively (Jonker and Picard, 2010 ; Carrique-Mas et al, 2014 ).…”

Section: Discussionmentioning

confidence: 98%

Prevalence, antimicrobial resistance and genetic diversity of Campylobacter coli and Campylobacter jejuni in Ecuadorian broilers at slaughter age

Vinueza-Burgos

Wautier²,

Martiny

et al. 2017

Poultry Science

View full text Add to dashboard Cite

Thermotolerant Campylobacter spp. are a major cause of foodborne gastrointestinal infections worldwide. The linkage of human campylobacteriosis and poultry has been widely described. In this study we aimed to investigate the prevalence, antimicrobial resistance and genetic diversity of C. coli and C. jejuni in broilers from Ecuador. Caecal content from 379 randomly selected broiler batches originating from 115 farms were collected from 6 slaughterhouses located in the province of Pichincha during 1 year. Microbiological isolation was performed by direct plating on mCCDA agar. Identification of Campylobacter species was done by PCR. Minimum inhibitory concentration (MIC) values for gentamicin, ciprofloxacin, nalidixic acid, tetracycline, streptomycin, and erythromycin were obtained. Genetic variation was assessed by RFLP-flaA typing and Multilocus Sequence Typing (MLST) of selected isolates. Prevalence at batch level was 64.1%. Of the positive batches 68.7% were positive for C. coli, 18.9% for C. jejuni, and 12.4% for C. coli and C. jejuni. Resistance rates above 67% were shown for tetracycline, ciprofloxacin, and nalidixic acid. The resistance pattern tetracycline, ciprofloxin, and nalidixic acid was the dominant one in both Campylobacter species. RFLP-flaA typing analysis showed that C. coli and C. jejuni strains belonged to 38 and 26 profiles respectively. On the other hand MLST typing revealed that C. coli except one strain belonged to CC-828, while C. jejuni except 2 strains belonged to 12 assigned clonal complexes (CCs). Furthermore 4 new sequence types (STs) for both species were described, whereby 2 new STs for C. coli were based on new allele sequences. Further research is necessary to estimate the impact of the slaughter of Campylobacter positive broiler batches on the contamination level of carcasses in slaughterhouses and at retail in Ecuador.

show abstract

Section: Discussionmentioning

confidence: 98%

Prevalence, antimicrobial resistance and genetic diversity of Campylobacter coli and Campylobacter jejuni in Ecuadorian broilers at slaughter age

Vinueza-Burgos

Wautier²,

Martiny

et al. 2017

Poultry Science

View full text Add to dashboard Cite

show abstract

“…A total of 152 thermotolerant Campylobacter were isolated from six poultry meat supply chains in Santa Fe region in Argentina (Zbrun et al, 2013). The stages sampled in each poultry meat chain were: a) hens from breeder flocks, b) broilers in flocks (aged <1 wk and >5 wk), c) chickens at the slaughterhouse, and d) chicken meat at the retail market.…”

Section: Collection Of Campylobacter Isolatesmentioning

confidence: 99%

Antimicrobial resistance in thermotolerant Campylobacter isolated from different stages of the poultry meat supply chain in Argentina

et al. 2015

View full text Add to dashboard Cite

“…Both between-and within-flock prevalence in Argentina was estimated based on data obtained by our group in different samplings (Rossler et al, 2020;Zbrun et al, 2013;Zbrun et al, 2017Zbrun et al, , 2021. The median estimate of transmission coefficient (β) was 1.54 ± 1.03 day -1 .…”

Section: Transmission Dynamics Of Thermotolerant Campylobacter In Com...mentioning

confidence: 99%

“…The vertical transmission of Campylobacter is not fully established, but current knowledge indicates that it has a very low probability of occurrence in poultry farms (Zhang and Sahin, 2020). In an observational study conducted by our group (Zbrun et al., 2013), we isolated Campylobacter in broiler birds that were less than one week of age (5–7 days). Humphrey et al.…”

Section: Introductionmentioning

confidence: 99%

Impact of farm‐level strategies against thermotolerant Campylobacter in broiler chickens, using a quantitative risk assessment model and meta‐analysis

Zbrun

Olivero

Soto

et al. 2022

Zoonoses and Public Health

View full text Add to dashboard Cite

The aim of this study was to evaluate the effect of control strategies (probiotic supplementation and vaccination) at farm on thermotolerant Campylobacter infection due to the consumption of salad cross‐contaminated from broiler meat. The broiler food chain was modelled considering the most common practices applied in Argentina (baseline model) and their effect on the prevalence and counts of Campylobacter. Probiotic supplementation and vaccination against Campylobacter spp. were included in different models to evaluate their effectiveness. The parameter distributions of each intervention were obtained based on a systematic review and meta‐analysis previously described. The control measures applied at farms were evaluated considering their effectiveness in reducing both the prevalence and the count of thermotolerant Campylobacter in comparison with the baseline model estimation and expressed as relative change in campylobacteriosis risk. Additionally, the identification of the most important input parameters for the model was performed by sensitivity analysis. The model estimated a risk of campylobacteriosis per consumed serving of salad contaminated with poultry meat of 4.99 x 10–3 (95% CI: 6.12 x 10–6–1.13 x 10–2), corresponding to an annual incidence risk estimated of 1,876,009 persons. Scenario analysis indicated that the application of vaccines against Campylobacter (probability of campylobacteriosis = 9.55 x 10–4; 95% CI: 5.31 x 10–4–1.29 x 10–3) and the supplementation of broilers with probiotics (probability of campylobacteriosis = 1.32 x 10–3; 8.55 x 10–4–1.69 x 10–3) can offer a modest reduction in risk estimates. The intervention efficacy was 80.86% and 73.54% for vaccination and probiotic supplementation, respectively. On‐farm interventions were effective to mitigate the risk of campylobacteriosis.

show abstract

Occurrence of thermotolerantCampylobacterspp. at different stages of the poultry meat supply chain in Argentina

Cited by 19 publications

References 26 publications

Prevalence, antimicrobial resistance and genetic diversity of Campylobacter coli and Campylobacter jejuni in Ecuadorian broilers at slaughter age

Prevalence, antimicrobial resistance and genetic diversity of Campylobacter coli and Campylobacter jejuni in Ecuadorian broilers at slaughter age

Antimicrobial resistance in thermotolerant Campylobacter isolated from different stages of the poultry meat supply chain in Argentina

Impact of farm‐level strategies against thermotolerant Campylobacter in broiler chickens, using a quantitative risk assessment model and meta‐analysis

Contact Info

Product

Resources

About