Occurrence of the Ordinary and the Andean Strains of Potato Virus S Infecting Potatoes in the Eastern Region of Kazakhstan

Khassanov, Vadim; Вологин, С Г

doi:10.1094/pdis-12-17-2000-pdn

Cited by 7 publications

(4 citation statements)

References 4 publications

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“…We next investigated whether our PVY‐resistant transgenic plants also cause interference with other potyviruses such as Potato virus A (PVA; He et al ., ) or unrelated viruses such as Potato virus S (PVS; Duan et al ., ; Khassanov and Vologin, ). After mechanical inoculation with PVS or PVA, transgenic plants and WT plants showed no significant difference in the accumulation of PVS (Figure e) or PVA (Figure f) viral particles.…”

Section: Resultsmentioning

confidence: 98%

Generation of virus‐resistant potato plants by RNA genome targeting

Zhan

Zhang

Zhong

et al. 2019

Plant Biotechnology Journal

138

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Summary CRISPR /Cas systems provide bacteria and archaea with molecular immunity against invading phages and foreign plasmids. The class 2 type VI CRISPR /Cas effector Cas13a is an RNA ‐targeting CRISPR effector that provides protection against RNA phages. Here we report the repurposing of CRISPR /Cas13a to protect potato plants from a eukaryotic virus, Potato virus Y ( PVY ). Transgenic potato lines expressing Cas13a/sg RNA (small guide RNA ) constructs showed suppressed PVY accumulation and disease symptoms. The levels of viral resistance correlated with the expression levels of the Cas13a/sg RNA construct in the plants. Our data further demonstrate that appropriately designed sg RNA s can specifically interfere with multiple PVY strains, while having no effect on unrelated viruses such as PVA or Potato virus S . Our findings provide a novel and highly efficient strategy for engineering crops with resistances to viral diseases.

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Section: Resultsmentioning

confidence: 98%

Generation of virus‐resistant potato plants by RNA genome targeting

Zhan

Zhang

Zhong

et al. 2019

Plant Biotechnology Journal

138

View full text Add to dashboard Cite

show abstract

“…Recent phylogenetic analyses show a clear separation between the geographical distribution of PVS isolates at the strain level (Duan et al, 2018;Santillan et al, 2018). PVS-O isolates are reported from all continents (Salari et al, 2011;Duan et al, 2018;Santillan et al, 2018) while PVS-A isolates are only reported from Asia, Oceania and South America (Cox and Jones, 2010;Duan et al, 2018;Khassanov and Vologin, 2018).…”

Section: Pest Distribution Outside the Eumentioning

confidence: 99%

Pest categorisation of potato virus S (non‐EU isolates)

Bragard¹,

Dehnen‐Schmutz²,

Gonthier³

et al. 2020

EFS2

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Following a request from the EU Commission, the Panel on Plant Health has addressed the pest categorisation of non-EU isolates of potato virus S (PVS). The information currently available on geographical distribution, biology, epidemiology, potential entry pathways, potential additional impact compared to the current situation in the EU, and availability of control measures of non-EU isolates of PVS has been evaluated with regard to the criteria to qualify as potential Union quarantine pest. Because non-EU isolates of PVS are absent from the EU, they do not meet one of the requirements to be regulated as an RNQP (presence in the EU); as a consequence, the Panel decided not to evaluate the other RNQP criteria for these isolates. Populations of PVS can be subdivided into two strains: the ordinary strain (PVS-O) with a worldwide distribution (including the EU), and the Andean strain (PVS-A) which is absent from the EU or considered to have at most a limited distribution in the EU. Two additional divergent isolates (PVS-A/PVS-O recombinants and PVS-arracacha) have also been categorised. Non-EU isolates of PVS-A are expected to have an additional impact as compared to the PVS isolates currently present in the EU, and therefore meet all the criteria to qualify as potential Union quarantine pests; the magnitude of the additional impact is, however, unknown. Non-EU isolates of PVS-A/PVS-O recombinants and of PVS-arracacha also meet these criteria, with the exception of the criterion regarding the potential additional consequences in the EU territory for which the Panel was unable to conclude. Non-EU PVS-O isolates are not expected to have an additional impact in the EU as compared to EU isolates and therefore do not meet the corresponding criterion.

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“…We next investigated whether our PVY-resistant transgenic plants also cause interference with other potyviruses such as Potato virus A (PVA; He et al, 2014) or unrelated viruses such as Potato virus S (PVS; Duan et al, 2018;Khassanov and Vologin, 2018). After mechanical inoculation with PVS or PVA, transgenic plants and WT plants showed no significant difference in the accumulation of PVS (Figure 5e) or PVA (Figure 5f) viral particles.…”

Section: Crispr/cas13a Specifically Targets Pvy Genomesmentioning

confidence: 99%

Faculty Opinions recommendation of Generation of virus-resistant potato plants by RNA genome targeting.

Jiang

2019

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature

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CRISPR/Cas systems provide bacteria and archaea with molecular immunity against invading phages and foreign plasmids. The class 2 type VI CRISPR/Cas effector Cas13a is an RNA-targeting CRISPR effector that provides protection against RNA phages. Here we report the repurposing of CRISPR/Cas13a to protect potato plants from a eukaryotic virus, Potato virus Y (PVY). Transgenic potato lines expressing Cas13a/sgRNA (small guide RNA) constructs showed suppressed PVY accumulation and disease symptoms. The levels of viral resistance correlated with the expression levels of the Cas13a/sgRNA construct in the plants. Our data further demonstrate that appropriately designed sgRNAs can specifically interfere with multiple PVY strains, while having no effect on unrelated viruses such as PVA or Potato virus S. Our findings provide a novel and highly efficient strategy for engineering crops with resistances to viral diseases.

show abstract

Occurrence of the Ordinary and the Andean Strains of Potato Virus S Infecting Potatoes in the Eastern Region of Kazakhstan

Cited by 7 publications

References 4 publications

Generation of virus‐resistant potato plants by RNA genome targeting

Generation of virus‐resistant potato plants by RNA genome targeting

Pest categorisation of potato virus S (non‐EU isolates)

Faculty Opinions recommendation of Generation of virus-resistant potato plants by RNA genome targeting.

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