Occurrence of sulfonamide and tetracycline-resistant bacteria and resistance genes in aquaculture environment

Gao, Panpan; Mao, Daqing; Luo, Yi; Wang, Limei; Xu, Bin; Xu, Lin

doi:10.1016/j.watres.2012.02.004

Cited by 566 publications

(203 citation statements)

References 54 publications

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“…Such widespread use of these antibiotics is most likely linked to the frequent isolation of strains resistant to TC and EM. Similar high rates of resistance to these antibiotics have also been reported for enterococcal strains isolated from rivers in other countries [53,54]. It was revealed that enterococci distributed in the studied urban river basin in this study are resistant to universally applicable antibiotics.…”

Section: Discussionsupporting

confidence: 87%

Antibiotic Resistance Profiling and Genotyping of Vancomycin-Resistant Enterococci Collected from an Urban River Basin in the Provincial City of Miyazaki, Japan

Nishiyama

Ogura

Hayashi

et al. 2017

Water

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Abstract:The distribution characteristics of vancomycin-resistant enterococci (VRE) and the resistance of enterococcus isolates to various antibiotics were investigated in Yae River, which flows through Miyazaki city, Japan. The prevalence of VRE among specimens collected from the urban river basin using mEI agar was 0.9% (2 of 226 enterococcal isolates). In the 333 enterococcal isolates obtained using mEI agar or vancomycin-supplemented mEI agar, the possession of the vancomycin-resistant genes (vanA, vanB, vanC1, and vanC2/C3) was examined using multiplex PCR analysis. Although VRE possessing vanA and vanB were not detected in any isolates, isolates possessing vanC2/C3 were detected at all sampling sites and on all days. All isolates (101 strains) possessing vanC2/C3 that were obtained on vancomycin-supplemented mEI agar were identified as E. casseliflavus and analyzed for genotypes using pulse-field gel electrophoresis (PFGE) analysis. These E. casseliflavus isolates revealed them to be genetically highly divergent strains, suggesting that many contamination sources were present in this study area. Many of the enterococcal isolates obtained were resistant to erythromycin, ciprofloxacin, and tetracycline; enterococci distributed in the studied urban river basin are resistant to universally applicable antibiotics. These results indicate that VRE carrying vanC2/C3 are distributed in Yae River, and the sources of VRE are scattered across the river basin.

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Section: Discussionsupporting

confidence: 87%

Antibiotic Resistance Profiling and Genotyping of Vancomycin-Resistant Enterococci Collected from an Urban River Basin in the Provincial City of Miyazaki, Japan

Nishiyama

Ogura

Hayashi

et al. 2017

Water

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“…Several studies have measured a resistance to SMX in bacteria isolated from biological WW treatment (Costanzo et al 2005;Reinthaler et al 2003), which has raised concerns regarding the prevalence of SMX in WWs leading to the development of bacterial resistance in the aquatic environment. These concerns have been validated recently by the detection of sulfonamide resistance genes in river water (which was correlated to the total SMX concentration among other sulfonamides) (Luo et al 2010) and the prevalence of SMX-resistant bacteria in aquaculture environments (Gao et al 2012b). …”

Section: Smx Usage and Presence In The Environmentmentioning

confidence: 99%

Biodegradation of sulfamethoxazole: current knowledge and perspectives

Larcher

Yargeau

2012

Appl Microbiol Biotechnol

114

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Antibiotic compounds, like sulfamethoxazole (SMX), have become a concern in the aquatic environment due to the potential development of antibacterial resistances. Due to extensive consumption, excretion and disposal, SMX has been frequently detected in wastewaters and surface waters. This has led to numerous studies investigating the nature of SMX, with many researchers focusing on the biodegradation and persistence of SMX during wastewater treatment and in the environment. This review provides a summary of recent developments, outlines the discrepancies in observations and results, and demonstrates the need for further research to determine optimal biological removal strategies for SMX and other antibiotics.

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“…Antibiotics affect the environmental microbiome and pose the problems of development and the propagation of antibiotic resistance (Gao et al 2012).…”

Section: Isolation Of Bacterial Endophytes From Phalaris Arundinacea mentioning

confidence: 99%

Isolation of Bacterial Endophytes from Phalaris arundinacea and their Potential in Diclofenac and Sulfamethoxazole Degradation

Węgrzyn

Felis

2018

Polish Journal of Microbiology

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A b s t r a c t Diclofenac (DCF), a non-steroidal anti-inflammatory drug (NSAID) and sulfamethoxazole (SMX), an antimicrobial agent, are in common use and can be often detected in the environment. The constructed wetland systems (CWs) are one of the technologies to remove them from the aquatic environment. The final effect of the treatment processes depends on many factors, including the interaction between plants and the plant-associated microorganisms present in the system. Bacteria living inside the plant as endophytes are exposed to secondary metabolites in the tissues. Therefore, they can possess the potential to degrade aromatic structures, including residues of pharmaceuticals. The endophytic strain MG7 identified as Microbacterium sp., obtained from root tissues of Phalaris arundinacea exposed to DCF and SMX was tested for the ability to remove 2 mg/l of SMX and DCF in monosubstrate cultures and in the presence of phenol as an additional carbon source. The MG7 strain was able to remove approximately 15% of DCF and 9% of SMX after 20 days of monosubstrate culture. However, a decrease in the optical density of the MG7 strain cultures was observed, caused by an insufficient carbon source for bacterial growth and proliferation. The adsorption of pharmaceuticals onto autoclaved cells was negligible, which confirmed that the tested strain was directly involved in the removal of DCF and SMX. In the presence of phenol as the additional carbon source, the MG7 strain was able to remove approximately 35% of DCF and 61% of SMX, while an increase in the optical density of the cultures was noted. The higher removal efficiency can be explained by adaptive mechanisms in microorganisms exposed to phenol (i.e. changes in the composition of membrane lipids) and by a co-metabolic mechanism, where non-growth substrates can be transformed by non-specific enzymes. The presence of both DCF and SMX and the influence of the supply frequency of CWs with the contaminated wastewater on the diversity of whole endophytic bacterial communities were demonstrated. The results of this study suggest the capability of the MG7 strain to degrade DCF and SMX. This finding deserves further investigations to improve wastewater treatment in CWs with the possible use of pharmaceuticals-degrading endophytes.K e y w o r d s: endophytic bacteria, constructed wetlands, diclofenac, sulfamethoxazole, biodegradation Węgrzyn A. and Felis E.

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Occurrence of sulfonamide and tetracycline-resistant bacteria and resistance genes in aquaculture environment

Cited by 566 publications

References 54 publications

Antibiotic Resistance Profiling and Genotyping of Vancomycin-Resistant Enterococci Collected from an Urban River Basin in the Provincial City of Miyazaki, Japan

Antibiotic Resistance Profiling and Genotyping of Vancomycin-Resistant Enterococci Collected from an Urban River Basin in the Provincial City of Miyazaki, Japan

Biodegradation of sulfamethoxazole: current knowledge and perspectives

Isolation of Bacterial Endophytes from Phalaris arundinacea and their Potential in Diclofenac and Sulfamethoxazole Degradation

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