Occurrence of ST23 Complex Phylogroup A
            <i>Escherichia coli</i>
            Isolates Producing Extended-Spectrum AmpC β-Lactamase in a French Hospital

Crémet, Lise; Caroff, Nathalie; Giraudeau, Cécile; Dauvergne, Sandie; Lepelletier, Didier; Reynaud, Alain; Corvec, Stéphane

doi:10.1128/aac.01580-09

Cited by 28 publications

(26 citation statements)

References 9 publications

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“…Thirteen isolates belonged to ST131, which includes a recently and globally emerging multidrug-resistant clone causing UTIs and septicemia in hospitals and the community. ST88 is correlated with extended-spectrum beta-lactamase (ESBL) production in nosocomial isolates (33,34). Three (23%) of the ST131 isolates and two (18%) of the ST88 strains were phenotypically ESBL positive.…”

Section: Resultsmentioning

confidence: 99%

“…Also, in our collection of urine isolates, only a relatively small proportion of ST95 isolates was identified. The ST88 (CC23) phylogroup C clone was recently associated with the spread of different beta-lactamases in nosocomial E. coli isolates (33,34). Also, E. coli of the phylogenetic lineages A and C that belong to CC10 and CC23 frequently caused extraintestinal infections in humans, and together with CC155 isolates, they are often multiresistant (44)(45)(46).…”

Section: Discussionmentioning

confidence: 99%

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Characterization of Escherichia coli Isolates from Hospital Inpatients or Outpatients with Urinary Tract Infection

et al. 2014

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e Uropathogenic Escherichia coli (UPEC) is the most common cause of community-and hospital-acquired urinary tract infections (UTIs). Isolates from uncomplicated community-acquired UTIs express a variety of virulence traits that promote the efficient colonization of the urinary tract. In contrast, nosocomial UTIs can be caused by E. coli strains that differ in their virulence traits from the community-acquired UTI isolates. UPEC virulence markers are used to distinguish these facultative extraintestinal pathogens, which belong to the intestinal flora of many healthy individuals, from intestinal pathogenic E. coli (IPEC). IPEC is a diarrheagenic pathogen with a characteristic virulence gene set that is absent in UPEC. Here, we characterized 265 isolates from patients with UTIs during inpatient or outpatient treatment at a hospital regarding their phylogenies and IPEC or UPEC virulence traits. Interestingly, 28 of these isolates (10.6%) carried typical IPEC virulence genes that are characteristic of enteroaggregative E. coli (EAEC), Shiga toxin-producing E. coli (STEC), and atypical enteropathogenic E. coli (aEPEC), although IPEC is not considered a uropathogen. Twenty-three isolates harbored the astA gene coding for the EAEC heat-stable enterotoxin 1 (EAST1), and most of them carried virulence genes that are characteristic of UPEC and/or EAEC. Our results indicate that UPEC isolates from hospital patients differ from archetypal community-acquired isolates from uncomplicated UTIs by their spectrum of virulence traits. They represent a diverse group, including EAEC, as well as other IPEC pathotypes, which in addition contain typical UPEC virulence genes. The combination of typical extraintestinal pathogenic E. coli (ExPEC) and IPEC virulence determinants in some isolates demonstrates the marked genome plasticity of E. coli and calls for a reevaluation of the strict pathotype classification of EAEC.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Characterization of Escherichia coli Isolates from Hospital Inpatients or Outpatients with Urinary Tract Infection

et al. 2014

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“…Together, the majority of the porcine ETEC isolates examined belonged to these three clonal complexes. A concern with the association of most porcine ETEC strains examined with the ST10 and ST23 complexes is that these complexes have been shown to be strongly associated with certain resistance-associated elements, such as AmpC-type beta-lactamases, NDM-type carbapenemases, and other extended-spectrum beta lactamases (7,9,32,37). Therefore, many porcine ETEC isolates may have an increased propensity to acquire and maintain these resistance-associated elements, as demonstrated by the genomes of UMNK88 and UMNF18.…”

Section: Resultsmentioning

confidence: 99%

Genome Sequences and Phylogenetic Analysis of K88- and F18-Positive Porcine Enterotoxigenic Escherichia coli

et al. 2012

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Porcine enterotoxigenic Escherichia coli (ETEC) continues to result in major morbidity and mortality in the swine industry via postweaning diarrhea. The key virulence factors of ETEC strains, their serotypes, and their fimbrial components have been well studied. However, most studies to date have focused on plasmid-encoded traits related to colonization and toxin production, and the chromosomal backgrounds of these strains have been largely understudied. Here, we generated the genomic sequences of K88-positive and F18-positive porcine ETEC strains and examined the phylogenetic distribution of clinical porcine ETEC strains and their plasmid-associated genetic content. The genomes of porcine ETEC strains UMNK88 and UMNF18 were both found to contain remarkable plasmid complements containing known virulence factors, potential novel virulence factors, and antimicrobial resistance-associated elements. The chromosomes of these strains also possessed several unique genomic islands containing hypothetical genes with similarity to classical virulence factors, although phage-associated genomic islands dominated the accessory genomes of these strains. Phylogenetic analysis of 78 clinical isolates associated with neonatal and porcine diarrhea revealed that a limited subset of porcine ETEC lineages exist that generally contain common toxin and fimbrial profiles, with many of the isolates belonging to the ST10, ST23, and ST169 multilocus sequencing types. These lineages were generally distinct from existing human ETEC database isolates. Overall, most porcine ETEC strains appear to have emerged from a limited subset of E. coli lineages that either have an increased propensity to carry plasmid-encoded virulence factors or have the appropriate ETEC core genome required for virulence. Diarrhea is a major cause of morbidity and mortality to young pigs, resulting in significant production losses for the swine industry (10). Enterotoxigenic Escherichia coli (ETEC) strains are the most common cause of diarrhea in young pigs, resulting in a disease known as enteric colibacillosis (10). Neonatal and postweaning pigs are most susceptible to ETEC. While neonatal ETEC diarrhea is generally well controlled by using fimbria-based vaccines, postweaning enteric colibacillosis has been more difficult to control. Porcine ETEC strains are characterized by the production of specific adhesins and enterotoxins. The adhesins most common in porcine ETEC are fimbrial adhesins K88 (also called F4), K99 (F5), 987P (F6), F41, and F18, as well as afimbrial adhesins such as AIDA-I. Porcine ETEC also produce one or more enterotoxins, including heat-labile enterotoxin (LT), heat-stable enterotoxins a and b (STa and STb), and EAST1 toxin (33). These and other described porcine ETEC virulence factors have mostly been localized to plasmids. Additionally, F18-positive porcine E. coli strains implicated in edema disease and diarrhea sometimes possess the Shiga toxin-encoding gene (stx 2e ) within their chromosomes (16).Analyses of human ETEC strains have revealed ...

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“…ST88 has also been described in association with chromosome-mediated AmpC overproduction in a French hospital (36), but so far its virulence profile had not been reported. Our ST88 isolates belonged to the recently proposed phylogroup C, and they possessed a high virulence score (mean, 9.2).…”

Section: Discussionmentioning

confidence: 99%

Inhibitor-Resistant TEM- and OXA-1-Producing Escherichia coli Isolates Resistant to Amoxicillin-Clavulanate Are More Clonal and Possess Lower Virulence Gene Content than Susceptible Clinical Isolates

Oteo

González-López

Ortega

et al. 2014

Antimicrob Agents Chemother

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In a previous prospective multicenter study in Spain, we found that OXA-1 and inhibitor-resistant TEM (IRT) ␤-lactamases constitute the most common plasmid-borne mechanisms of genuine amoxicillin-clavulanate (AMC) resistance in Escherichia coli. In the present study, we investigated the population structure and virulence traits of clinical AMC-resistant E. coli strains expressing OXA-1 or IRT and compared these traits to those in a control group of clinical AMC-susceptible E. coli isolates. All OXA-1-producing (n ‫؍‬ 67) and IRT-producing (n ‫؍‬ 45) isolates were matched by geographical and temporal origin to the AMCsusceptible control set (n ‫؍‬ 56). We performed multilocus sequence typing and phylogenetic group characterization for each isolate and then studied the isolates for the presence of 49 virulence factors (VFs) by PCR and sequencing. The most prevalent clone detected was distinct for each group: group C isolates of sequence type (ST) 88 (C/ST88) were the most common in OXA-1 producers, B2/ST131 isolates were the most common in IRT producers, and B2/ST73 isolates were the most common in AMCsusceptible isolates. The median numbers of isolates per ST were 3.72 in OXA-1 producers, 2.04 in IRT producers, and 1.69 in AMC-susceptible isolates; the proportions of STs represented by one unique isolate in each group were 19.4%, 31.1%, and 48.2%, respectively. The sum of all VFs detected, calculated as a virulence score, was significantly higher in AMC-susceptible isolates than OXA-1 and IRT producers (means, 12.5 versus 8.3 and 8.2, respectively). Our findings suggest that IRT-and OXA-1-producing E. coli isolates resistant to AMC have a different and less diverse population structure than AMC-susceptible clinical E. coli isolates. The AMC-susceptible population also contains more VFs than AMC-resistant isolates.

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Occurrence of ST23 Complex Phylogroup A Escherichia coli Isolates Producing Extended-Spectrum AmpC β-Lactamase in a French Hospital

Cited by 28 publications

References 9 publications

Characterization of Escherichia coli Isolates from Hospital Inpatients or Outpatients with Urinary Tract Infection

Characterization of Escherichia coli Isolates from Hospital Inpatients or Outpatients with Urinary Tract Infection

Genome Sequences and Phylogenetic Analysis of K88- and F18-Positive Porcine Enterotoxigenic Escherichia coli

Inhibitor-Resistant TEM- and OXA-1-Producing Escherichia coli Isolates Resistant to Amoxicillin-Clavulanate Are More Clonal and Possess Lower Virulence Gene Content than Susceptible Clinical Isolates

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