Occurrence of rare genotypes of Streptococcus agalactiae in cultured red tilapia Oreochromis sp. and Nile tilapia O. niloticus in Thailand—Relationship to human isolates?

Suanyuk, Naraid; Kong, Fanrong; Ko, Danny; Gilbert, Gwendolyn L.; Supamattaya, K.

doi:10.1016/j.aquaculture.2008.07.034

Cited by 147 publications

(127 citation statements)

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“…One hundred and forty-four GBS isolates from infected tilapia obtained from different farms, between 2003 and 2011, were used in this study. Of these 144 GBS isolates, 74 were obtained from a collection previously evaluated by our group (Suanyuk et al, 2008) and 70 were obtained from infected tilapia from the present study (Fig. 1).…”

Section: Methodsmentioning

confidence: 99%

“…The serotype of GBS was determined using Group B Streptococci Typing antisera (Denka Seiken). The experiments were quality controlled and validated with the GBS DMST17129 and GBS PSU-KSAAHRC-ST81 isolates from infected tilapia cultured in Nakhon Si Thammarat Province (Suanyuk et al, 2008).…”

Section: Methodsmentioning

confidence: 99%

“…Since then, in Thailand, the disease has been reported in Asian seabass (Lates calcarifer) and tilapia (Direkbusarakom & Donayado, 1987;Wanman et al, 2005;Kitancharoen et al, 2006;Suanyuk et al, 2008Suanyuk et al, , 2010. Streptococcosis of tilapia caused by GBS and S. iniae have been reported (Kitancharoen et al, 2006;Suanyuk et al, 2008Suanyuk et al, , 2010 and to our knowledge, GBS is the most common species isolated from infected tilapia in Thailand.…”

Section: Introductionmentioning

confidence: 99%

“…GBS is an emerging zoonotic agent isolated from terrestrial and aquatic animals (Martinez et al, 2000;Duremdez et al, 2004;Bishop et al, 2007;Evans et al, 2008;Suanyuk et al, 2008;Fischer et al, 2013). In order to treat bacterial diseases in cultured tilapia, oxytetracycline is permitted for use in Thai aquaculture (Baoprasertkul et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

“…The major pathogenic bacteria responsible for mortalities in tilapia include Aeromonas hydrophila (Tipmongkolsilp et al, 2012), Francisella noatunensis subsp. orientalis (Soto et al, 2013), Flavobacterium columnare (Dong et al, 2015), Vibrio vulnificus (Chen et al, 2006), Streptococcus iniae (Suanyuk et al, 2010) and Streptococcus agalactiae (group B Streptococcus, GBS) (Suanyuk et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

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Serotype distribution and antimicrobial susceptibilities of Streptococcus agalactiae isolated from infected cultured tilapia (Oreochromis niloticus) in Thailand: Nine-year perspective

Dangwetngam

Suanyuk

Kong

et al. 2016

Journal of Medical Microbiology

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Streptococcus agalactiae (group B Streptococcus, GBS) infection remains a major problem associated with high mortality of cultured tilapia worldwide. The present study reports the serotype distribution and antimicrobial susceptibilities of GBS isolated from infected tilapia cultured in Thailand. One hundred and forty-four GBS isolates were identified by biochemical, serological and molecular analyses. Of these 144 GBS isolates, 126 were serotype Ia and 18 were serotype III. Antimicrobial susceptibilities of the 144 GBS isolates were determined by the disc diffusion method. Most GBS isolates were susceptible to lincomycin, norfloxacin, oxytetracycline, ampicillin, erythromycin and chloramphenicol, but resistant to oxolinic acid, gentamicin, sulfamethoxazole and trimethoprim. However, 17 isolates displayed an oxytetracycline-resistant phenotype and harboured the tet(M) gene. The broth microdilution method was used to determine the minimal inhibitory concentrations (MICs) of 17 oxytetracycline-resistant GBS isolates, and then minimal bactericidal concentrations (MBCs) of these isolates were evaluated. Oxytetracyline-resistant isolates were found to be susceptible to ampicillin, lincomycin, norfloxacin, erythromycin and chloramphenicol, with the MIC and MBC ranging from j0.125 to 0.5 mg ml 21 and j0.125 to 2 mg ml 21 , respectively. Moreover, all 17 oxytetracycline-resistant isolates demonstrated resistance to trimethoprim, oxolinic acid, gentamicin, sulfamethoxazole and oxytetracycline, with the MIC and MBC ranging from 16 to $128 mg ml 21 and $128 mg ml 21, respectively. These findings are useful information for antibiotic usage in fish aquaculture.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%