Occurrence of races and pathotypes of cucurbit powdery mildew in southeastern Spain

Pino, Daniel Del; Olalla, Lucı́a; Pérez-Garcı́a, Alejandro; Rivera, María Eugenia Sánchez Díaz de; García, Sara Mallén; Moreno, R.; Vicente, Antonio de; Torés, J. A.

doi:10.1007/bf02979750

Cited by 59 publications

(40 citation statements)

References 13 publications

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“…2002). Although two fungal species, Golovinomyces cichoracearum and Podosphaera fusca , are responsible for the disease, only the latter has been identified as the etiological agent of cucurbit powdery mildew in Spain (del Pino et al. 2002; Fernández‐Ortuño et al.…”

Section: Introductionmentioning

confidence: 99%

Effect of lipopeptides of antagonistic strains ofBacillus subtilison the morphology and ultrastructure of the cucurbit fungal pathogenPodosphaera fusca

Romero

Vicente

Olmos

et al. 2007

Journal of Applied Microbiology

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111

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Aims: To analyse the morphological and ultrastructural effects of lipopeptides of cell‐free liquid cultures from the antagonistic Bacillus subtilis strains, UMAF6614 and UMAF6639, on the cucurbit powdery mildew fungus, Podosphaera fusca, conidial germination. Methods and Results: Butanolic extracts from cell‐free supernatants of B. subtilis cultures were tested for their ability to arrest P. fusca conidial germination using the zucchini cotyledon disc method. Previously, the occurrence of lipopeptide antibiotics fengycin, iturin/bacillomycin and surfactin in the extracts was verified by diverse chromatographic approaches. Conidial germination was strongly reduced by antifungal extracts obtained from liquid cultures of both B. subtilis strains. Scanning electron microscopy analysis showed morphological damage in conidia characterized by the presence of large depressions and loss of turgidness. Transmission electron microscopy analysis revealed severe modifications in the plasma membrane and disorganization of the P. fusca cell cytoplasm. Conclusions: The lipopeptides produced by the two strains of B. subtilis are able to reduce cucurbit powdery mildew disease by arresting conidial germination, which seems to result from the induction of important cytological alterations. Significance and Impact of the Study: We elucidated the mechanisms employed by these antagonistic strains of B. subtilis to suppress cucurbit powdery mildew disease and delineate the ultrastructural damages responsible for their suppressive effect.

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Section: Introductionmentioning

confidence: 99%

Effect of lipopeptides of antagonistic strains ofBacillus subtilison the morphology and ultrastructure of the cucurbit fungal pathogenPodosphaera fusca

Romero

Vicente

Olmos

et al. 2007

Journal of Applied Microbiology

Self Cite

111

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“…Nevertheless, as a few spores viable would be enough to get a couple of powdery mildew colonies, it is likely that survival periods longer than 5 years could be achieved with this method, like the one described for B. graminis , which has been tried up to 10 years (L. Munk, personal communication). Furthermore, phenotypic stability of the isolates was analysed by testing specific traits, such as race‐specific avirulence and host range (Del Pino et al., 2002). No differences were observed between data recorded before and after storage (data not shown).…”

Section: Resultsmentioning

confidence: 99%

“…Erysiphe cichoracearum ), species that induce identical symptoms but can be distinguished easily under light microscopy (Braun et al., 2002). In southern Spain, however, P. fusca has been identified as the sole cause of powdery mildew in cucurbits (Del Pino et al., 2002). Studies on population genetics or fungicide resistance of this fungus are essential to establish better control measures.…”

Section: Introductionmentioning

confidence: 99%

Long‐term Preservation of Podosphaera fusca Using Silica Gel

Pérez-Garcı́a¹,

Mingorance²,

Rivera³

et al. 2006

Journal of Phytopathology

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Podosphaera fusca is the main causal agent of cucurbit powdery mildew in Spain and one of the most important limiting factors for cucurbit production worldwide. As an obligate biotrophic parasite, this fungus has been traditionally cultured and conserved by periodical transfers of conidia to fresh plant material. Here we describe a simple protocol for preservation of P. fusca isolates in absence of living tissue based on the dry spore, slow-freezing technique, and demonstrate that storage of silica gel desiccated conidia at )80°C is an efficient method for the long-term preservation of the pathogen.www.blackwell-synergy.com

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“…Two differential sets for the determination of pathotypes have been proposed (Bertrand 1991;del Pino et al 2002;Lebeda et al 2008) (Table 1). Bertrand (1991) assembled the most frequently used differential set for pathotype determination; it included one genotype from each of four species representing three agriculturally important cucurbit genera plus two genotypes from a fifth species, melon Cucumis melo L. (Table 1).…”

Section: Pathotype Determinationmentioning

confidence: 99%

Gaps and perspectives of pathotype and race determination in Golovinomyces cichoracearum and Podosphaera xanthii

et al. 2011

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Golovinomyces cichoracearum and Podosphaera xanthii (family Erysiphaceae) are the most important species causing cucurbit powdery mildew (CPM), a serious disease of field and greenhouse cucurbits. Both species are highly variable in their pathogenicity and virulence, as indicated by the existence of large number of different pathotypes and races. Various independent systems of CPM pathotype and race determinations and denominations are used worldwide. CPM pathotype identification is based on intergeneric and interspecific differences in host-CPM interactions. The most commonly used set of CPM pathotype differentials includes one genotype from four species representing three agriculturally important cucurbit genera plus two genotypes from a fifth species, melon Cucumis melo L. CPM races are characterized by specialization on different cultivars or lines of one host species and have, to date, been differentiated only on melon (C. melo L.). The most frequently used set of melon differentials includes 11 genotypes that can differentiate CPM races originating from melon and other cucurbits, e.g., cucumber, Cucurbita spp., and watermelon. In this paper, we critically review the current state, gaps, and perspectives in our understanding of pathogenicity variation in these two CPM pathogens at the pathotype and race levels.

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Occurrence of races and pathotypes of cucurbit powdery mildew in southeastern Spain

Cited by 59 publications

References 13 publications

Effect of lipopeptides of antagonistic strains ofBacillus subtilison the morphology and ultrastructure of the cucurbit fungal pathogenPodosphaera fusca

Effect of lipopeptides of antagonistic strains ofBacillus subtilison the morphology and ultrastructure of the cucurbit fungal pathogenPodosphaera fusca

Long‐term Preservation of Podosphaera fusca Using Silica Gel

Gaps and perspectives of pathotype and race determination in Golovinomyces cichoracearum and Podosphaera xanthii

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