Occurrence of Psorospermium haeckeli in the stone crayfish Austropotamobius torrentium from a population naturally mixed with the noble crayfish Astacus astacus

Vogt, Günter; Keller, Martina; Brandis, Dirk

doi:10.3354/dao025233

Cited by 9 publications

(5 citation statements)

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“…may have a diphasic life cycle, and although a putative intermediate host has not been demonstrated, it can be transmitted from P. leniusculus to A. astacus and between P. leniusculus individuals but not from A. astacus to P. leniusculus (Gydemo, 1996). However, Vogt et al (1996) suggested that Psorospermium was transmissible from A. astacus to Austropotamobius torrentium. A free living stage without the need for an intermediate host may exist (Vogt and Rug, 1999).…”

Section: Mesomycetozoeamentioning

confidence: 89%

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Diseases of crayfish: A review

Longshaw

2011

Journal of Invertebrate Pathology

129

112

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Section: Mesomycetozoeamentioning

confidence: 89%

“…Psorospermium haeckeli is elongate, slightly spindle shaped with roundish or lance-like ends and measures 120-180 Â 50 Â 50 lm, the other type being egg-shaped and measuring $100 Â 60 Â 60 lm (Vogt and Rug, 1995). Both types cooccur in several crayfish species (Henttonen et al, 1997;Vogt et al, 1996;Rug, 1995, 1999).…”

Section: Mesomycetozoeamentioning

confidence: 95%

Diseases of crayfish: A review

Longshaw

2011

Journal of Invertebrate Pathology

129

112

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“…Although P. haeckeli was detected in the stone crayfish A. torrentium by other authors (VEY, 1979), especially when it coexisted with the noble crayfish A. astacus (VOGT, KELLER and BRANDIS, 1996) we have never found infected specimens in this species. One reason could be that many tissue specimens were not fresh but were preserved in ethanol.…”

Section: Discussionmentioning

confidence: 62%

“…Most publications dealing with Psorospermium describe these organisms as being relatively large (often over 100 µm long), oval or elongated, filled with globules of different sizes within an outer thick wall or capsule with a verticular pattern (BOSHKO, 1981;NYLUND, WESTMAN and LOUNATMAA, 1983). Many isolated observations of P. haeckeli have been reported in crayfish from different areas in Europe (HENTTONEN, HUNER and LINDQVIST, 1995;CERENIUS and SÖDERHÄLL, 1993;NOVOSELTCEV, PEPELYAEV and HENTTONEN, 1995;TAUGBØL and SKURDAL, 1992;BUCINSKIENE, 1996;VOGT, KELLER and BRANDIS, 1996;DIÉGUEZ-URIBEONDO et al, 1993). However, studies of the organism's distribution in the southeast of the continent are lacking.…”

Section: Introductionmentioning

confidence: 99%

Occurence of the Pathogen Psorospermium Haeckeli (Hilgendorf) in Astacid Populations in Croatia

Lučić

Maguire

Erben

2004

Bull. Fr. Pêche Piscic.

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Psorospermium haeckeli is a thick-walled unicellular organism widely reported in European astacid crayfish. It is typically referred to as a "parasite" although its pathogenic impact remains unclear. Until now there were no data about its presence in Croatian astacid crayfish. We examined 144 specimens of the three astacid species from 12 different locations and found sporocysts of Psorospermium in 13 preparations of epidermal connective tissue of Astacus astacus and Astacus leptodactylus. Preparations were made by scraping the epidermal-connective tissue from under the carapace, treating it with 10% KOH and viewing it under a microscope at 125×, 250× and 400× magnification. A relative scale was used to describe the degree of infection of the crayfish by Psorospermium. We found two types of mature sporocysts and also some developing stages of Psorospermium. In the A. leptodactylus we found many sporocysts that were fully melanised. Further investigations are needed to understand the distribution of infected crayfish in Croatia and patterns of their infection with Psorospermium. Key words: Psorospermium haeckeli, Astacus astacus, Astacus leptodactylus, Austropotamobius torrentium, Croatia APPARITION DU PATHOGÈNE PSOROSPERMIUM HAECKELI (HILGENDORF) DANS LES POPULATIONS D'ÉCREVISSES EN CROATIE RÉSUMÉPsorospermium haeckeli est un organisme unicellulaire à parois épaisse très répandu chez les écrevisses européennes. P. haeckeli est considéré comme un « parasite » même si sa pathogénicité reste encore non clairement définie. Jusqu'à présent il n'y avait aucune donnée concernant sa présence chez les écrevisses en Croatie. Nous avons examiné 144 spécimens de trois espèces d'écrevisses provenant de 12 stations et trouvé des sporocystes de P. Haeckeli dans 13 préparations de tissu conjonctif épidermique prélevé sous la carapace, préparé avec du KOH 10 %, et observé sous microscope aux grossissements 125×, 250× et 400×. Une échelle relative a été utilisée pour décrire le degré d'infection des écrevisses par P. haeckeli. Nous avons trouvé deux types de sporocystes de P. haeckeli matures et quelques-uns en développement. Chez A. leptodactylus nous avons trouvé plusieurs sporocystes complètement mélanisés. De plus amples recherches sont nécessaires pour comprendre la distribution des écrevisses infectées en Croatie et le mode d'infection de Psorospermium.

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“…Of these, 2 morphotypes are found in Europe , the third morphotype is present in North America (Henttonen et al 1992(Henttonen et al , 1994 and the fourth one is found in Australia (Herbert 1987). The different morphotypes have been suggested to constitute different species , Vogt et al 1996. However, whether different morphotypes are different strains or species is still not resolved.…”

mentioning

confidence: 97%

Sequence analysis of the ribosomal internal transcribed spacer DNA of the crayfish parasite Psorospermium haeckeli

Bangyeekhun¹,

Ryynänen²,

Henttonen³

et al. 2001

Dis. Aquat. Org.

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Two morphotypes of the crayfish parasite Psorospermium haeckeli were isolated from 2 crayfish species of different geographical origin. The oval-shaped sporocysts were obtained from the epidermal and connective tissue beneath the carapace of the noble crayfish Astacus astacus from Sweden and Finland. Elongated spores were isolated from the abdominal muscle tissue of the red swamp crayfish Procambarus clarkii from USA. To compare genetic divergence of 2 morphotypes of the parasite, the ribosomal internal transcribed spacer (ITS) DNA (ITS 1 and ITS 2) and the 5.8S rRNA gene were cloned and sequenced. The analysed region is variable in length, with the ribosomal ITS sequence of the European morphotype longer than the North American one. Sequence diversity is found mainly in ITS 1 and ITS 2 regions, and there is 66% and 58% similarity between the 2 morphotypes, respectively. Thus, analysis of the ribosomal ITS DNA suggests that P. haeckeli forms obtained from Europe and North America are genetically diverse, which supports the previously reported morphological characteristics.KEY WORDS: Psorospermium haeckeli · Astacus astacus · Procambarus clarkii · Crayfish · Parasite · Ribosomal RNA · Internal transcribed spacer Resale or republication not permitted without written consent of the publisherDis Aquat Org 46: [217][218][219][220][221][222] 2001 amoeboid form of the parasite. Analysis of the nuclearencoded small subunit (SSU) rRNA gene of P. haeckeli showed that it belongs to a phylogenetic clade near the animal-fungal dichotomy (Ragan et al. 1996, Baker et al. 1999, Herr et al. 1999, Benny & O'Donnell 2000, Figueras et al. 2000, Ustinova et al. 2000, and it was suggested that this novel taxon be named Ichthyosporea according to its class status (Cavalier-Smith 1998). Like P. haeckeli, the members of this clade, except for Amoebidium parasiticum, are unicellular parasites of animals, and no complete life cycle of any species is known (Ragan et al. 1996, Herr et al. 1999.At least 4 morphotypes of Psorospermium haeckeli have been described, as determined by size, morphology and histology. It seems likely that the occurrence of different morphotypes may be linked to crayfish species of different geographical origin. Of these, 2 morphotypes are found in Europe , the third morphotype is present in North America (Henttonen et al. 1992(Henttonen et al. , 1994 and the fourth one is found in Australia (Herbert 1987). The different morphotypes have been suggested to constitute different species , Vogt et al. 1996. However, whether different morphotypes are different strains or species is still not resolved. The DNA sequences of non-coding regions of the large pre-rRNA coding segment, i.e., internal transcribed spacer (ITS) 1 and 2, are less conserved than the regions-encoding genes and have been used to distinguish between strains or species of organisms (Hillis & Dixon 1991). We analysed the nucleotide sequence of the ribosomal ITS DNA (ITS 1, 5.8S rRNA and ITS 2) from 2 morphotypes of P. haeckeli, isolated from ...

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Occurrence of Psorospermium haeckeli in the stone crayfish Austropotamobius torrentium from a population naturally mixed with the noble crayfish Astacus astacus

Abstract: NOTEOccurrence of Psorospermium haeckeli in the stone crayfish Austropotamobius torrentium from a population naturally mixed with the noble crayfish Astacus astacusGiinter v o g t l , Max ell er^, Dirk rand is' '~eparhnent of Zoology 1

Cited by 9 publications

References 9 publications

Diseases of crayfish: A review

Diseases of crayfish: A review

Occurence of the Pathogen Psorospermium Haeckeli (Hilgendorf) in Astacid Populations in Croatia

Sequence analysis of the ribosomal internal transcribed spacer DNA of the crayfish parasite Psorospermium haeckeli

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