Occurrence of protochlorophyll and its phototransformation to chlorophyll in mutant C‐2A’ of <i>Scenedesmus obliquus</i>

Kotzabasis, Kiriakos; Schüring, Max-Peter; Senger, Horst

doi:10.1111/j.1399-3054.1989.tb06172.x

Cited by 22 publications

(7 citation statements)

References 24 publications

(14 reference statements)

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“…1). The earlier results obtained by TLC separation of PCh!(Kotzabasis et al 1989) are in good agreement with the ones presented here when it is considered that the DV-derivative makes up only about 1/4 of the whole PChl as can be seen in the elution profile of the HPLC-separation(Fig. \).…”

supporting

confidence: 92%

“…Pigments were extracted by phase partitioning with diethyl ether to remove water soluble pigments and proteins from the extract (Me Ewen and Lindsten 1992) and transferred into acetone. The PChl fraction was obtained by separation on Fractogel TSK DEAE-650 (S) as described earlier (Kotzabasis et al 1989). Analysis of different esterification stages was investigated by HPLC following Boddi et al, (1989) and Me Ewen and Lindsten (1992) but extending tbe gradient lo 30 titin.…”

Section: Determination Of Different Esterification Stages Of Mvand DVmentioning

confidence: 99%

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Monovinyl and divinyl protochlorophyll in different stages of esterification isolated from mutant C-2A' of the unicellular green alga Scenedesmus obliquus

Knaust¹,

Senger²

1994

Physiol Plant

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Knaust, R, and Senger, H, 1994, Monovinyl and divinyl protochlorophyil in different stages of esterification isolated from the mutant C-2X of the unicellular green alga Seenedesmus ohiii/ttus. -Physiol, Plant, 90: 490-496,The mutant C-2.A' of the unicellular green alga Scenedesmus obtiqutis accumulates the chlorophyll-precursors protochlorophyllide and the already esterified protochlorophyll when grown heterotrophically in the dark. Two derivatives of protochlorophyll, monovinyl protochlorophyll (MV-PChl) and divinyl protochlorophyll (DV-PChS), were isolated from dark-grown cells of mutant C-2A' and characterized by absorption and fluorescence spectroscopy. Their tnolecular masses were determined by plasma desorption mass spectrometry. Both MV-and DV-PChl were mainly esterified vvith geranylgeraniol. iiowever, some esterification with the tnore saturated alcohoEs dihydrogeranylgeraniol, tetrahydrogeranylaeraniol and phytol could aiso be detected.

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supporting

confidence: 92%

Section: Determination Of Different Esterification Stages Of Mvand DVmentioning

confidence: 99%

Monovinyl and divinyl protochlorophyll in different stages of esterification isolated from mutant C-2A' of the unicellular green alga Scenedesmus obliquus

Knaust¹,

Senger²

1994

Physiol Plant

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“…Esterified Chl that was found shortly after illumination has been addressed either as the product of phototransformation of esterified protochlorophyll (Pchl; Lancer et al 1976;Kotzabasis et al 1989) or as the product of rapid esterification (Adra and Rebeiz 1998;Domanskii and Ru¨diger 2001). To decide between these possibilities, we analyzed the esterified pigments in the etiolated leaves before and immediately after the flash (Fig.…”

Section: Resultsmentioning

confidence: 99%

Characterization of two phases of chlorophyll formation during greening of etiolated barley leaves

Domanskii

Rassadina

Gus-Mayer

et al. 2003

Planta

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The esterification kinetics of chlorophyllide, obtained by a single flash of light, were investigated in etiolated barley (Hordeum vulgare L.) and oat (Avena sativa L.) leaves. A rapid phase, leading to esterification of 15% of total chlorophyllide within 15-30 s, was followed by a lag-phase of nearly 2 min and a subsequent main phase, leading to esterification of 85% of total chlorophyllide within 30-60 min. The presence of additional protochlorophyllide, produced in the leaves by incubation with 5-aminolevulinate, did not change the esterification kinetics. The rapid phase was identical after partial (11-15%) and full (>80%) photoconversion of protochlorophyllide; the ability for a second rapid esterification phase was restored in a dark period of at least 10 min. Cooling the leaves to 0°C abolished the esterification of the main phase while the rapid phase remained unchanged. The prolamellar bodies were already in part transformed into prothylakoid-like structures within 2-5 min after a full flash but not after a weak flash (11% photoconversion); in the latter case, the corresponding transformation required a dark period of about 45 min. The existence of subcomplexes of prolamellar bodies containing NADPH:protochlorophyllide oxidoreductase and chlorophyll synthase in the ratio 7:1 is discussed.

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“…This group should be a propionic acid holding a free carboxylic group at the C17 2 position (Fig. Pchlide esterified with long-chain alcohol, however, has been reported to occur as substrate of LPOR isolated from the mutant C-2A of the green microalgae Scenedesmus obliquus (104). Longer alcohol chains like phytol (96,101,102) or an acrylic group (36,103) prevent photoreduction.…”

Section: Substrate Specificity Of Lpormentioning

confidence: 99%

Protochlorophyllide Reduction: Mechanisms and Evolution¶

Schoefs¹,

Franck²

2003

Photochem Photobiol

138

120

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Protochlorophyllide (Pchlide) reductases are key enzymes in the process of chlorophyll biosynthesis. In this review, current knowledge on the molecular organization, substrate specificity and assembly of the light-dependent reduced nicotinamide adenine dinucleotide phosphate:Pchlide oxidoreductases are discussed. Characteristics of light-independent enzymes are also described briefly, and the possible reasons for the selection of light-dependent enzymes during the course of evolution are discussed.

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Occurrence of protochlorophyll and its phototransformation to chlorophyll in mutant C‐2A’ of Scenedesmus obliquus

Cited by 22 publications

References 24 publications

Monovinyl and divinyl protochlorophyll in different stages of esterification isolated from mutant C-2A' of the unicellular green alga Scenedesmus obliquus

Monovinyl and divinyl protochlorophyll in different stages of esterification isolated from mutant C-2A' of the unicellular green alga Scenedesmus obliquus

Characterization of two phases of chlorophyll formation during greening of etiolated barley leaves

Protochlorophyllide Reduction: Mechanisms and Evolution¶

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