Occurrence of plasmid-mediated quinolone resistance genes in Pseudomonas aeruginosa strains isolated from clinical specimens in southwest Iran: a multicentral study

Saki, Morteza; Sheikh, Ahmad Farajzadeh; Seyed‐Mohammadi, Sakineh; Dezfuli, Aram Asareh Zadegan; Shahin, Mojtaba; Tabasi, Maryam; Veisi, Hojat; Keshavarzi, Raziyeh; Khani, Parisa

doi:10.1038/s41598-022-06128-4

Cited by 13 publications

(12 citation statements)

References 37 publications

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“…In this study, PDC β-lactamase and OXA β-lactamase genes were prevalent in collected strains, which can induce multidrug resistance to some extent by antibiotic inactivation. Previous studies showed that the presence of qnrVC genes plays important roles in quinolone resistance (Liu et al, 2018;Saki et al, 2022), and 2.3% of collected Heat map showing the resistance genes for the collected P. aeruginosa high-risk clone strains. The heat map was formed by performing tblastn searches using the sequences present on the Comprehensive Antibiotic Resistance Database (CARD) as queries and the genomes as subjects.…”

Section: Discussionmentioning

confidence: 99%

Whole-genome sequencing reveals high-risk clones of Pseudomonas aeruginosa in Guangdong, China

Zhao

Chen

et al. 2023

Front. Microbiol.

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The ever-increasing prevalence of infections produced by multidrug-resistant or extensively drug-resistant Pseudomonas aeruginosa is commonly linked to a limited number of aptly-named epidemical ‘high-risk clones’ that are widespread among and within hospitals worldwide. The emergence of new potential high-risk clone strains in hospitals highlights the need to better and further understand the underlying genetic mechanisms for their emergence and success. P. aeruginosa related high-risk clones have been sporadically found in China, their genome sequences have rarely been described. Therefore, the large-scale sequencing of multidrug-resistance high-risk clone strains will help us to understand the emergence and transmission of antibiotic resistances in P. aeruginosa high-risk clones. In this study, 212 P. aeruginosa strains were isolated from 2 tertiary hospitals within 3 years (2018–2020) in Guangdong Province, China. Whole-genome sequencing, multi-locus sequence typing (MLST) and antimicrobial susceptibility testing were applied to analyze the genomic epidemiology of P. aeruginosa in this region. We found that up to 130 (61.32%) of the isolates were shown to be multidrug resistant, and 196 (92.45%) isolates were Carbapenem-Resistant Pseudomonas aeruginosa. MLST analysis demonstrated high diversity of sequence types, and 18 reported international high-risk clones were identified. Furthermore, we discovered the co-presence of exoU and exoS genes in 5 collected strains. This study enhances insight into the regional research of molecular epidemiology and antimicrobial resistance of P. aeruginosa in China. The high diversity of clone types and regional genome characteristics can serve as a theoretical reference for public health policies and help guide measures for the prevention and control of P. aeruginosa resistance.

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Section: Discussionmentioning

confidence: 99%

Whole-genome sequencing reveals high-risk clones of Pseudomonas aeruginosa in Guangdong, China

Zhao

Chen

et al. 2023

Front. Microbiol.

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“…Recently several studies have reported the presence of qnr genes in P. aeruginosa. Saki et al (2022) from Iran reported the findings of qnr B, A and S in 29%, 25% and 20% of isolates, respectively. From saudi, qnr D, S and AAC(6)-Ib-cr were found in 79%, 79% and 71% of P. aeruginosa; the other study from India showed that 78% of isolates had aac(6′)-Ib-cr, 14% of isolates harbored qnrB and qnrS, 10% had both qnrB and qnrS and 3% oqxAB gene (El-Badawy et al, 2019;Geetha et al, 2022).…”

Section: Plasmid-mediated Quinolone Resistancementioning

confidence: 98%

Antimicrobial resistance in Pseudomonas aeruginosa

Mohanam¹,

Shanmugam²

2022

MJM

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In recent years, antimicrobial resistance (AMR) has become a global public health concern. The growth of resistant bacteria is increasing dramatically, while the number of new antibiotics accessible is decreasing. This is especially true in the case of Pseudomonas aeruginosa, an important causative agent of healthcare-associated infections. The ability of P. aeruginosa to survive in different environments and on medical devices has made it more resistant to antibiotics. This causes bacteremia in hospitalized patients, ventilator-associated pneumonia, catheter-associated urinary tract infections and wound infections, particularly in patients with severe burns, bed ulcers and immunocompromised individuals. The rise in the AMR rate in both developed and developing countries may be attributed to a number of factors such as variations in the standard health care, large population, awareness about antibiotic resistance, inadequate training on rationale antibiotic usage and inadequate infection control facilities in many hospitals. The emergence of Extensive Drug Resistance (XDR) and Pan Drug Resistance (PDR) among organisms that cause various infections leads to increased treatment costs, morbidity and mortality, leaving no therapeutic options. This review highlights the different mechanisms of antibiotic resistance, including intrinsic and acquired resistance, which are frequently observed in P. aeruginosa.

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“…The compounds in this class inhibit two bacterial enzymes that play an essential role in bacterial DNA synthesis. Resistance to quinolones is recognized as a shared mechanism contributing to resistance in Gram-negative bacilli such as E. coli and P. aeruginosa through protein modification (Abdel-Rhman et al, 2021 ; Saki et al, 2022 ). Sulfonamides are one of the oldest types of antibiotics that belong to the class of synthetic antimicrobial agents.…”

Section: Emergence Of Antibiotic Resistancementioning

confidence: 99%

Review of hospital effluents: special emphasis on characterization, impact, and treatment of pollutants and antibiotic resistance

Fatimazahra¹,

Mouhir²,

Saafadi³

et al. 2023

Environ Monit Assess

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Health care institutions generate large volumes of liquid effluents from specific activities related to healthcare, analysis, and research. Their direct discharge into the environment has various negative effects on aquatic environments and human health, due to their high organic matter charges and the presence of various emerging contaminants such as disinfectants, drugs, bacteria, viruses, and parasites. Moreover, hospital effluents, by carrying antibiotics, contribute to the development of antibiotic-resistant microorganisms in the environment. This resistance has become a global issue that manifests itself variously in different countries, causing the transmission of different infections. In this respect, an effort is provided to protect water resources by current treatment methods that imply physical–chemical processes such as adsorption and advanced oxidation processes, biological processes such as activated sludge and membrane bioreactors and other hybrid techniques. The purpose of this review is to improve the knowledge on the composition and impact of hospital wastewater on man and the environment, highlighting the different treatment techniques appropriate to this type of disposal before discharge into the environment.

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Occurrence of plasmid-mediated quinolone resistance genes in Pseudomonas aeruginosa strains isolated from clinical specimens in southwest Iran: a multicentral study

Cited by 13 publications

References 37 publications

Whole-genome sequencing reveals high-risk clones of Pseudomonas aeruginosa in Guangdong, China

Whole-genome sequencing reveals high-risk clones of Pseudomonas aeruginosa in Guangdong, China

Antimicrobial resistance in Pseudomonas aeruginosa

Review of hospital effluents: special emphasis on characterization, impact, and treatment of pollutants and antibiotic resistance

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