Occurrence of plasmid DNA in serologically defined strains of Campylobacter jejuni and Campylobacter coli

Bradbury, W C; Marko, M A; Hennessy, J N; Penner, J L

doi:10.1128/iai.40.2.460-463.1983

Cited by 45 publications

(23 citation statements)

References 22 publications

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“…Even this drastic measure may not solve the problem, however, as plasmids may appear in a higher size range than 125 kb, either because they actually are that large ("megaplasmids") or because they have not been digested by the restriction enzyme used and are not in a linear conformation (Barton et al, 1995). It is wellknown that plasmids, including megaplasmids, are common in foodborne pathogens (Bradbury et al, 1983;Wachsmuth et al, 1991;Olsen et al, 1994;Barton et al, 1995;Iteman et al, 1996;McLauchlin et al, 1997), but the influence of the plasmid content on the PFGE profiles of foodborne pathogens has not been well characterized.…”

Section: Tenover Criteria and Sources Of Variability In Pfge Patternsmentioning

confidence: 99%

Interpretation of Pulsed-Field Gel Electrophoresis Patterns in Foodborne Disease Investigations and Surveillance

Barrett

Gerner‐Smidt

Swaminathan

2006

Foodborne Pathogens and Disease

169

107

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Since the establishment of the well-known Tenover criteria in 1995 (Tenover et al., 1995), relatively few papers have been published about the interpretation of subtyping data generated by pulsed-field gel electrophoresis (PFGE). This paper describes the approach that has been used in the PulseNet network during the past 10 years. PFGE data must always be interpreted in the proper epidemiological context and PFGE data can not alone prove an epidemiological connection. The Tenover criteria are not generally applicable to the interpretation of PFGE subtyping data of foodborne pathogens. The reproducibility of the method with a particular organism, the quality of the PFGE gel, the variability of the organism being subtyped, and the prevalence of the pattern in question must always be considered. Only isolates displaying indistinguishable patterns should be included in the detection of clusters of infections or the initial case definition in a point-source outbreak. More variability (patterns differing from each other in two to three band positions) may be accepted if the outbreak has been going on for some time or if person-person spread is a prominent feature. If epidemiological information is sufficiently strong, isolates with markedly different PFGE patterns may be included in an outbreak.

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Section: Tenover Criteria and Sources Of Variability In Pfge Patternsmentioning

confidence: 99%

Interpretation of Pulsed-Field Gel Electrophoresis Patterns in Foodborne Disease Investigations and Surveillance

Barrett

Gerner‐Smidt

Swaminathan

2006

Foodborne Pathogens and Disease

169

107

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“…Following the procedure of KADO and LIU (17), with some modifications (4,7,9,27), several large colonies from overnight cultures on brain-heart infusion agar or sheep blood agar were suspended in 20 p1 of E-solution (40 mM tris p H 7.9 + 2 mM EDTA) to which 100 p1 of lysis solution (3 % SDS + 50mM trisacetate in 100ml distilled water (pH 12.2) were added. The solution was emulsified by brief shaking and gentle inversion for several times.…”

Section: Preparation Of Plasmid Dnamentioning

confidence: 99%

Plasmids and Resistance to 9 Chemotherapeutic Agents of Pasteurella multocida and Pasteurella haemolytica Epidemiological Aspects

Haghour

Hellmann

Schmidt

1987

Journal of Veterinary Medicine, Series B

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Summary A total of 163 strains of P. multocida and 60 of P. haemolytica from different animal species, which had been isolated from 1966 to 1986 in 2 European and 2 Asian countries were investigated for plasmids and for antimicrobial resistance. The agarose gel electrophoresis of alkaline extracted DNA showed that 35 P. multocida (21.5%) and 26 P. haemolytica strains (43.3%) carried plasmid DNA. In all but one strain only one plasmid was seen. The molecular size of the plasmids ranged from 1.3 to 28.8 Megadalton (Md), but small plasmids of ≤ 3.4 Md prevailed in both species. 108 P. multocida (66.3%) and all P. haemolytica strains were resistant to one or more of the nine chemotherapeutic agents tested. Distinct differences according to the origin of strains were observed. Most frequently, resistance occurred to Oxacillin, Erythromycin and Streptomycin. Except for 20 plasmid‐carrying cattle strains of P. haemolytica which showed, in addition to the chemotherapeutics mentioned above, resistance to Ampicillin, Penicillin G, Tetracycline and Sulfamethoxazol‐Trimethoprim, no other antimicrobial resistance seemed to be associated with the presence of plasmid DNA. Zusammenfassung Plasmidvorkommen und Resistenz gegen 9 Chemotherapeutika von Pasteurella multocida und Pasteurella haemolytica unter epidemiologischen Aspekten 163 P. multocida und 60 P. haemolytica‐Stämme, die von 1966 bis 1986 von verschiedenen Tierarten isoliert worden waren und aus 2 europäischen und 2 asiatischen Ländern stammten, wurden auf das Vorhandensein von Plasmiden und auf ihre Chemotherapeutika‐Resistenz untersucht. Die Gel‐Elektrophorese von alkalisch‐extrahierter DNA zeigte, daß 35 P. multocida‐Stämme (21,5%) und 26 P. haemolytica‐Stämme (43,3%) Plasmid‐DNA enthielten; mit Ausnahme eines Stammes wurde lediglich ein Plasmid ermittelt. Die Molekülgröße der Plasmide lag zwischen 1,3–28,8 Megadalton (Mdal), doch herrschten kleine Plasmide ≤ 3,4 Mdal in beiden Spezies vor. 108 P. multocida‐Stämme (66,3%) und alle P. haemolytica‐Stämme waren gegen ein oder mehrere von 9 überprüften Chemotherapeutika resistent. Es gab deutliche Unterschiede in Abhängigkeit

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“…are being developed: that of Lior et al (17) dealing with C. jejiuni, in which 14 to 17% of the strains examined were found to be untypable, and another system devised with both C. jejluni and C. coli in mind (21). Certain difficulties with the latter system have been encountered, and it has been suggested that plasmid typing may be necessary as an additional means of discrimination (5).…”

mentioning

confidence: 99%

Identification of Campylobacter coli isolates from animals and humans by bacterial restriction endonuclease DNA analysis

Kakoyiannis¹,

Winter²,

Marshall³

1984

Appl Environ Microbiol

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Ninety-nine Campylobacter coli isolates were examined by bacterial restriction endonuclease DNA analysis (BRENDA) with HindIII. Isolates from poultry from the same environment had identical patterns, patterns of isolates carried by suckling piglets were generally the same as those of isolates recovered from their dams, and one human patient yielded the same BRENDA type when sampled 6 weeks later. The 14 human isolates examined produced 11 distinct BRENDA types. Forty-three C. coli isolates from pigs were represented by 20 BRENDA types. Ten C. coli isolates from the feces of gulls yielded five different BRENDA types. Thirty-two C. coli isolates from live chickens and processed chicken yielded five different BRENDA types. Three human isolates had identical DNA patterns; two were from brothers living in the same house, and the third was from a human with no apparent relationship to the brothers. Another human isolate was identical to a poultry isolate. None of the pig strains had DNA patterns resembling those of human strains, nor were the DNA patterns like those of any strains recovered from poultry or gulls. Four C. coli isolates were subcultured onto agar 23 times over a period of 45 days, and their BRENDA patterns were preserved. BRENDA shows great promise for use in epidemiological studies of C. coli. Campvlobacter (oli was first described as Vibrio coli (7) and was thought to be the etiological agent of swine dysentry (6). Veron and Chatelain (36) classified the genus Cainpylobacteer and renamed V. coli as C. coli. This renaming has recently been accepted by the International Committee on Systematic Bacteriology (27). The feature which distinguishes C. coli from C. jejiuni is its inability to hydrolyze hippurate in the test described by Harvey (9) and Skirrow and Benjamin (30). Although C. (oli is less frequently isolated from cases of human diarrhea than is C. jejiunii (3, 4. 11, 14, 22, 31), there appears to be no obvious difference in disease severity for humans with either of these two species (29). Each year in New Zealand a number of acute enteritis cases in humans are found to be caused by C. (oli (D. M. Norris, personal communication), but their source is un-* Corresponding author.

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Occurrence of plasmid DNA in serologically defined strains of Campylobacter jejuni and Campylobacter coli

Cited by 45 publications

References 22 publications

Interpretation of Pulsed-Field Gel Electrophoresis Patterns in Foodborne Disease Investigations and Surveillance

Interpretation of Pulsed-Field Gel Electrophoresis Patterns in Foodborne Disease Investigations and Surveillance

Plasmids and Resistance to 9 Chemotherapeutic Agents of Pasteurella multocida and Pasteurella haemolytica Epidemiological Aspects

Identification of Campylobacter coli isolates from animals and humans by bacterial restriction endonuclease DNA analysis

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