Occurrence of phosphonolipidsin mycobacteria

Sarma, G.R.K.; Chandramouli, Vaishnavi; Venkitasubramanian, T. A.

doi:10.1016/0005-2760(70)90024-x

Cited by 18 publications

(5 citation statements)

References 16 publications

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“…In various lower animal species, compounds with a carbon-to-phosphorus bond occur free and have been shown to be abundant constituents of lipids (12) and recently of a surface polysaccharide (13). Evidence has been presented for the existence of small amounts of 2-aminoethylphosphonate in acid hydrolysates of total lipid from several species of mycobacteria; the nature of the parent substance was not identified (23). To our knowledge there have been no other reports of phosphonolipids in bacteria.…”

Section: Resultsmentioning

confidence: 87%

Occurrence of Phosphonosphingolipids in Bdellovibrio bacteriovorus Strain UKi2

1973

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The major phospholipids of two strains of Bdellovibrio bacteriovorus were characterized. Both strain UKil, which is obligately saprophytic, and strain UKi2, which is facultatively parasitic, contained phosphatidylethanolamine and phosphatidylglycerol as their major glycerophosphatides. A branched, 15-carbon fatty acid is the major component of these alkali-labile lipids. Absent from UKil but present in UKi2 were three alkali-stable lipids (compounds 8, 9, and 11) which appear to be phosphosphingolipids. After acid hydrolysis, both compound 8 and 9 yield the identical phosphorus-containing substance that is water soluble, dipolar ionic, and ninhydrin positive. This substance appears to contain a C-P bond since P1 could not be released from this substance by treatment with alkaline phosphatase or by very harsh mineral acid treatment. Based on chromatographic comparisons, this phosphonate appears to be a novel lipid constituent. Upon degradation, compound 8 yields 1 mol of dihydroxy long-chain base and compound 9 yields 1 mol of a trihydroxy long-chain base. These bases appear to have a 17-carbon, possibly branched, structure based on gas-liquid chromatography retention times. Degradation of both sphingolipids yields a mixture of hydroxy fatty acids, the major component being a branched, 15-carbon hydroxy acid. 1971).MATERIALS AND METHODS Growth of bacteria. B. bacteriovorus strains UKil and UKi2 (4) were grown on 1% (wt/vol) peptone-0.3% (wt/vol) yeast extract on a rotary shaker at 30 C for 15 h. In some experiments the medium was supplemented with "2P-orthophosphate (carrier-free, New England Nuclear Corp., or ICN Corp.).Extraction of lipid. The phospholipids were extracted according to a modification of the method of Lester and Steiner (17). '2P-lieled cells were harvested from 25 ml of medium by centrifugation and suspended in 1.0 ml of water. A 5-ml amount of (CH2CH2)20-CH,CH2OH (3:1, vol/vol) was added, on July 16, 2020 by guest

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Section: Resultsmentioning

confidence: 87%

Occurrence of Phosphonosphingolipids in Bdellovibrio bacteriovorus Strain UKi2

1973

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“…Mycobacteria contain no phosphatidylcholines (lecithins) (88,193). Evidence of the presumed phosphonic acid analogs of PE has been deduced from isolation of the definitive fragment-2 aminoethylphosphonic acid (213).…”

Section: Phospholipidsmentioning

confidence: 99%

Mycobacterial lipids: selected topics.

Goren¹

1972

Bacteriological Reviews

105

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“…The ubiquitous nature of AEP in biological tissues such as protozoa, Coelenterates, Molluscs, Echinoderms, Arthropods, Vertebrates, Schizomycophytes and Thallophytes was reported by Horiguchi (1971). Detectable amounts of AEP also have been found in goat liver (Kandatsu and Horiguchi, 1965), bovine brain (Shimizu et al, 1965), mycobacteria (Sarma et al, 1970), bile of the bovine (Tamari and Kametaka, 1973), bovine milk (Tamari and Kandatsu, 1985) and human tissues (Alhadeft and Daves, 1971). Because AEP has been detected in many biological materials, it is not unusual that AEP was found in substantial quantities in some feedstuffs and ruminal bacteria.…”

Section: Resultsmentioning

confidence: 99%

Occurrence of 2-Aminoethylphosphonic Acid in Feeds, Ruminal Bacteria and Duodenal Digesta from Defaunated Sheep

Ankrah

Loerch

Dehority

1989

Journal of Animal Science

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A quantitative method of analysis for 2-aminoethylphosphonic acid (AEP) was developed using reverse-phase HPLC. The detection limit for AEP was 15 nM, and the detector response (peak area) was linear from AEP levels up to 100 microM (R = .99). Mean recovery of AEP added to strained ruminal fluid from faunated sheep was 98.2%. When AEP was added to a fermentation mixture at a concentration of 22.6 micrograms/ml, 78% disappeared during a 24-h incubation. 2-Aminoethylphosphonic acid was readily detected in preparations of mixed ruminal ciliate protozoa as well as in mixed and pure strains of ruminal bacteria, feedstuffs, and ruminal fluid and duodenal digesta from defaunated sheep. The occurrence of AEP in feed and bacterial hydrolysates was confirmed by organic phosphorus analyses. The concentration of AEP in mixed ruminal protozoa was three times greater than its concentration in mixed ruminal bacteria (4,304 vs 1,383 micrograms/g DM, respectively). The AEP values for pure ruminal bacterial cultures ranged from 733 micrograms/g DM in Bacteroides succinogenes B21a to 1,166 micrograms/g DM in Butyrivibrio fibrisolvens H17c. Ruminal fluid and duodenal digesta from defaunated sheep contained AEP concentrations of 30 micrograms/ml and 90 micrograms/g DM, respectively. The concentration of AEP in feedstuffs ranged from 25 micrograms/g DM in wheat straw to 263 micrograms/g DM in oats. Because AEP occurrence is not limited to ruminal ciliate protozoa, it is of little value as a marker for protozoal presence in or passage out of the rumen.

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Occurrence of phosphonolipidsin mycobacteria

Cited by 18 publications

References 16 publications

Occurrence of Phosphonosphingolipids in Bdellovibrio bacteriovorus Strain UKi2

Occurrence of Phosphonosphingolipids in Bdellovibrio bacteriovorus Strain UKi2

Mycobacterial lipids: selected topics.

Occurrence of 2-Aminoethylphosphonic Acid in Feeds, Ruminal Bacteria and Duodenal Digesta from Defaunated Sheep

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